WDR45L (WDR45B) (NM_019613) Human Tagged ORF Clone
CAT#: RG203196
- TrueORF®
WDR45B (tGFP-tagged) - Human WDR45-like (WDR45L)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_019613" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | WDR45L |
Synonyms | NEDSBAS; WDR45L; WIPI-3; WIPI3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203196 representing NM_019613
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTATTTCGCTGCAACTATTTAGCTTTAGTTGGTGGTGGAAAAAAGCCGAAATACCCTCCCAACAAAG TAATGATCTGGGATGACCTGAAGAAGAAGACTGTTATTGAAATAGAATTTTCTACAGAAGTCAAGGCAGT CAAACTGCGGCGAGATAGAATTGTGGTGGTTTTGGACTCCATGATTAAGGTGTTCACATTCACACACAAT CCCCATCAGTTGCACGTCTTCGAAACCTGCTATAACCCCAAAGGCCTCTGTGTCCTTTGTCCCAATAGTA ACAACTCCCTCCTGGCCTTTCCGGGCACGCACACGGGCCATGTGCAGCTTGTGGACCTGGCCAGCACGGA GAAGCCACCCGTGGACATTCCTGCACACGAGGGTGTCCTGAGCTGCATTGCACTCAACCTGCAGGGAACA AGAATTGCAACTGCATCCGAGAAAGGGACGCTTATAAGAATATTTGATACTTCATCAGGGCATTTAATCC AGGAACTGCGAAGAGGATCTCAAGCAGCCAATATTTACTGCATCAACTTCAATCAGGATGCGTCCCTCAT CTGCGTATCCAGCGACCACGGCACAGTGCATATTTTTGCAGCTGAAGATCCAAAAAGGAATAAACAGTCC AGTTTGGCCTCAGCCAGTTTCCTTCCAAAATACTTCAGTTCCAAGTGGAGTTTCTCCAAGTTTCAGGTTC CCTCAGGCTCTCCGTGCATTTGTGCCTTTGGAACAGAGCCAAACGCCGTCATTGCAATTTGTGCAGACGG CAGCTACTACAAATTCCTGTTCAACCCCAAGGGGGAGTGCATCCGAGATGTCTACGCGCAGTTTCTAGAG ATGACCGATGACAAGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203196 representing NM_019613
Red=Cloning site Green=Tags(s) MLFRCNYLALVGGGKKPKYPPNKVMIWDDLKKKTVIEIEFSTEVKAVKLRRDRIVVVLDSMIKVFTFTHN PHQLHVFETCYNPKGLCVLCPNSNNSLLAFPGTHTGHVQLVDLASTEKPPVDIPAHEGVLSCIALNLQGT RIATASEKGTLIRIFDTSSGHLIQELRRGSQAANIYCINFNQDASLICVSSDHGTVHIFAAEDPKRNKQS SLASASFLPKYFSSKWSFSKFQVPSGSPCICAFGTEPNAVIAICADGSYYKFLFNPKGECIRDVYAQFLE MTDDKL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_019613 |
ORF Size | 858 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019613.2, NP_062559.1 |
RefSeq Size | 2596 bp |
RefSeq ORF | 1035 bp |
Locus ID | 56270 |
UniProt ID | Q5MNZ6 |
Cytogenetics | 17q25.3 |
Domains | WD40 |
Gene Summary | This gene encodes a member of the WIPI or SVP1 family of WD40 repeat-containing proteins. The protein contains seven WD40 repeats that are thought to fold into a beta-propeller structure that mediates protein-protein interactions, and a conserved motif for interaction with phospholipids. The human genome contains several pseudogenes of this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203196 | WDR45B (Myc-DDK-tagged)-Human WDR45-like (WDR45L) |
USD 300.00 |
|
RC203196L3 | Lenti ORF clone of Human WDR45-like (WDR45L), Myc-DDK-tagged |
USD 600.00 |
|
RC203196L4 | Lenti ORF clone of Human WDR45-like (WDR45L), mGFP tagged |
USD 600.00 |
|
SC317535 | WDR45B (untagged)-Human WDR45-like (WDR45L) |
USD 503.00 |
|
SC320157 | WDR45B (untagged)-Human WDR45-like (WDR45L) |
USD 457.00 |
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