WDR45L (WDR45B) (NM_019613) Human Tagged ORF Clone

CAT#: RC203196

WDR45B (Myc-DDK-tagged)-Human WDR45-like (WDR45L)

Product Images

Circular map for RC203196

• 

  Western blot validation of overexpression lysate (Cat# LY412737) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203196 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR45L
• Synonyms: NEDSBAS; WDR45L; WIPI-3; WIPI3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203196 representing NM_019613
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTATTTCGCTGCAACTATTTAGCTTTAGTTGGTGGTGGAAAAAAGCCGAAATACCCTCCCAACAAAG
  TAATGATCTGGGATGACCTGAAGAAGAAGACTGTTATTGAAATAGAATTTTCTACAGAAGTCAAGGCAGT
  CAAACTGCGGCGAGATAGAATTGTGGTGGTTTTGGACTCCATGATTAAGGTGTTCACATTCACACACAAT
  CCCCATCAGTTGCACGTCTTCGAAACCTGCTATAACCCCAAAGGCCTCTGTGTCCTTTGTCCCAATAGTA
  ACAACTCCCTCCTGGCCTTTCCGGGCACGCACACGGGCCATGTGCAGCTTGTGGACCTGGCCAGCACGGA
  GAAGCCACCCGTGGACATTCCTGCACACGAGGGTGTCCTGAGCTGCATTGCACTCAACCTGCAGGGAACA
  AGAATTGCAACTGCATCCGAGAAAGGGACGCTTATAAGAATATTTGATACTTCATCAGGGCATTTAATCC
  AGGAACTGCGAAGAGGATCTCAAGCAGCCAATATTTACTGCATCAACTTCAATCAGGATGCGTCCCTCAT
  CTGCGTATCCAGCGACCACGGCACAGTGCATATTTTTGCAGCTGAAGATCCAAAAAGGAATAAACAGTCC
  AGTTTGGCCTCAGCCAGTTTCCTTCCAAAATACTTCAGTTCCAAGTGGAGTTTCTCCAAGTTTCAGGTTC
  CCTCAGGCTCTCCGTGCATTTGTGCCTTTGGAACAGAGCCAAACGCCGTCATTGCAATTTGTGCAGACGG
  CAGCTACTACAAATTCCTGTTCAACCCCAAGGGGGAGTGCATCCGAGATGTCTACGCGCAGTTTCTAGAG
  ATGACCGATGACAAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203196 representing NM_019613
  Red=Cloning site Green=Tags(s)
  
  MLFRCNYLALVGGGKKPKYPPNKVMIWDDLKKKTVIEIEFSTEVKAVKLRRDRIVVVLDSMIKVFTFTHN
  PHQLHVFETCYNPKGLCVLCPNSNNSLLAFPGTHTGHVQLVDLASTEKPPVDIPAHEGVLSCIALNLQGT
  RIATASEKGTLIRIFDTSSGHLIQELRRGSQAANIYCINFNQDASLICVSSDHGTVHIFAAEDPKRNKQS
  SLASASFLPKYFSSKWSFSKFQVPSGSPCICAFGTEPNAVIAICADGSYYKFLFNPKGECIRDVYAQFLE
  MTDDKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019613
• ORF Size: 858 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019613.2, NP_062559.1
• RefSeq Size: 2596 bp
• RefSeq ORF: 1035 bp
• Locus ID: 56270
• UniProt ID: Q5MNZ6
• Cytogenetics: 17q25.3
• Domains: WD40
• MW: 37.9 kDa
• Gene Summary: This gene encodes a member of the WIPI or SVP1 family of WD40 repeat-containing proteins. The protein contains seven WD40 repeats that are thought to fold into a beta-propeller structure that mediates protein-protein interactions, and a conserved motif for interaction with phospholipids. The human genome contains several pseudogenes of this gene. [provided by RefSeq, Jul 2008]