WDR45L (WDR45B) (NM_019613) Human Untagged Clone

CAT#: SC317535

WDR45B (untagged)-Human WDR45-like (WDR45L)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WDR45L
• Synonyms: NEDSBAS; WDR45L; WIPI-3; WIPI3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317535 representing NM_019613.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAACCTCCTGCCGTGTAACCCTCACGGCAACGGGCTGCTCTACGCCGGCTTCAACCAGGACCACGGA
  TGCTTTGCGTGTGGGATGGAAAATGGATTCCGAGTCTATAACACTGATCCACTAAAAGAAAAAGAGAAA
  CAAGAATTTCTAGAAGGAGGAGTTGGCCATGTTGAAATGTTATTTCGCTGCAACTATTTAGCTTTAGTT
  GGTGGTGGAAAAAAGCCGAAATACCCTCCCAACAAAGTAATGATCTGGGATGACCTGAAGAAGAAGACT
  GTTATTGAAATAGAATTTTCTACAGAAGTCAAGGCAGTCAAGCTGCGGCGAGATAGAATTGTGGTGGTT
  TTGGACTCCATGATTAAGGTGTTCACATTCACACACAATCCCCATCAGTTGCACGTCTTCGAAACCTGC
  TATAACCCCAAAGGCCTCTGTGTCCTTTGTCCCAATAGTAACAACTCCCTCCTGGCCTTTCCGGGCACG
  CACACGGGCCATGTGCAGCTTGTGGACCTGGCCAGCACGGAGAAGCCACCCGTGGACATTCCTGCACAC
  GAGGGTGTCCTGAGCTGCATTGCACTCAACCTGCAGGGAACAAGAATTGCAACTGCATCCGAGAAAGGG
  ACGCTTATAAGAATATTTGATACTTCATCAGGGCATTTAATCCAGGAACTGCGAAGAGGATCTCAAGCA
  GCCAATATTTACTGCATCAACTTCAATCAGGATGCGTCCCTCATCTGCGTATCCAGCGACCACGGCACA
  GTGCATATTTTTGCAGCTGAAGATCCAAAAAGGAATAAACAGTCCAGTTTGGCCTCAGCCAGTTTCCTT
  CCAAAATACTTCAGTTCCAAGTGGAGTTTCTCCAAGTTTCAGGTTCCCTCAGGCTCTCCGTGCATTTGT
  GCCTTTGGAACAGAGCCAAACGCCGTCATTGCAATTTGTGCAGACGGCAGCTACTACAAATTCCTGTTC
  AACCCCAAGGGGGAGTGCATCCGAGATGTCTACGCGCAGTTTCTAGAGATGACCGATGACAAGCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_019613
• Insert Size: 1035 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019613.3
• RefSeq Size: 2596 bp
• RefSeq ORF: 1035 bp
• Locus ID: 56270
• UniProt ID: Q5MNZ6
• Cytogenetics: 17q25.3
• Domains: WD40
• MW: 38.1 kDa
• Gene Summary: This gene encodes a member of the WIPI or SVP1 family of WD40 repeat-containing proteins. The protein contains seven WD40 repeats that are thought to fold into a beta-propeller structure that mediates protein-protein interactions, and a conserved motif for interaction with phospholipids. The human genome contains several pseudogenes of this gene. [provided by RefSeq, Jul 2008]