RAGEF2 (RAPGEF6) (NM_001164387) Human Tagged ORF Clone

CAT#: RC228682

RAPGEF6 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant 3

Product Images

Circular map for RC228682

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RAGEF2
• Synonyms: KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2; RAGEF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228682 representing NM_001164387
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCACCCGTGGACCCTGGCGCTAGGCAGGCGTTGAGGAAGAAGCCACCCGAGCGGACTCCCGAGG
  ACTTAAATACTATTTATTCTTATCTTCATGGAATGGAAATATTATCAAATCTCAGGGAACATCAGCTTAG
  ATTAATGTCTGCAAGAGCACGCTATGAGAGATACAGTGGCAATCAGGTTCTCTTTTGTTCAGAAACGATT
  GCCAGATGTTGGTATATCCTACTTTCTGGATCTGTGCTTGTGAAAGGCTCCATGGTCTTGCCTCCTTGCA
  GTTTTGGTAAGCAGTTTGGAGGAAAAAGAGGATGTGATTGTCTTGTATTAGAGCCTTCAGAAATGATTGT
  GGTAGAGAATGCCAAAGATAATGAAGATAGTATTCTACAAAGAGAAATTCCTGCCAGACAATCCCGAAGA
  AGATTTCGGAAAATTAACTATAAAGGAGAGCGCCAAACCATTACTGATGATGTGGAGGTTAACAGCTATC
  TTTCTCTTCCAGCTGATCTTACCAAGATGCATCTCACAGAAAACCCTCATCCACAGGTGACTCATGTGTC
  TTCTAGTCAGTCTGGTTGTAGCATTGCCAGTGACTCTGGAAGCAGCAGTTTATCTGATATCTATCAGGCT
  ACGGAGAGTGAGGTAGGAGATGTAGATTTGACACGTCTTCCAGAAGGACCTGTTGATTCTGAGGATGACG
  AAGAGGAAGATGAAGAGATTGATCGAACAGATCCATTGCAGGGGCGAGATCTTGTTCGAGAATGTCTTGA
  AAAAGAACCTGCAGACAAAACTGATGATGACATTGAACAATTGCTGGAGTTTATGCACCAGCTCCCTGCA
  TTTGCAAACATGACCATGTCTGTAAGGAGAGAACTCTGCTCAGTGATGATTTTTGAAGTGGTAGAGCAGG
  CTGGAGCTATTATTCTTGAAGATGGGCAAGAGCTTGACTCATGGTATGTTATTTTAAACGGCACTGTGGA
  AATCAGTCATCCAGATGGAAAAGTTGAAAATTTGTTTATGGGAAATAGTTTTGGAATTACTCCCACTCTG
  GATAAGCAGTACATGCATGGAATTGTCAGGACTAAAGTAGATGATTGTCAGTTTGTCTGCATAGCCCAGC
  AAGATTATTGGAGAATTTTAAACCATGTGGAAAAAAATACCCATAAAGTTGAGGAAGAGGGAGAAATTGT
  TATGGTACATGAGCATCGGGAACTAGACCGGAGTGGAACCAGGAAAGGACACATTGTGATCAAGGCAACA
  CCTGAGCGTCTCATAATGCATTTAATAGAAGAACATTCCATCGTGGATCCAACTTATATAGAAGATTTTC
  TATTAACTTACAGGACATTTCTTGAAAGTCCTTTGGATGTTGGGATCAAACTATTGGAATGGTTTAAGAT
  CGACAGCTTAAGAGATAAGGTGACACGGATTGTATTATTATGGGTAAATAATCATTTTAATGATTTTGAA
  GGTGACCCTGCTATGACTCGATTTCTAGAGGAATTTGAAAAAAATCTGGAAGATACAAAGATGAATGGTC
  ATCTCCGGTTATTGAATATTGCCTGTGCTGCAAAGGCTAAGTGGAGACAGGTTGTGCTGCAAAAGGCTTC
  CCGCGAGTCCCCTCTACAATTCAGCCTTAATGGAGGGAGTGAGAAGGGATTTGGTATTTTTGTTGAAGGA
  GTAGAACCTGGTAGCAAAGCTGCTGATTCAGGACTGAAACGTGGTGATCAGATTATGGAAGTAAATGGAC
  AAAACTTTGAGAATATTACATTTATGAAAGCCGTTGAAATTTTGAGGAATAATACTCATCTTGCACTTAC
  TGTGAAGACCAACATTTTTGTGTTCAAAGAGTTACTTTTTAGGACTGAACAAGAGAAATCTGGTGTTCCT
  CATATTCCCAAAATTGCTGAAAAAAAAAGTAATCGCCATTCTATCCAGCATGTGCCAGGAGATATTGAAC
  AGACATCACAGGAGAAAGGAAGTAAGAAAGTTAAAGCAAATACTGTTTCAGGTGGAAGAAACAAAATCAG
  GAAGATTTTGGATAAAACACGATTTAGTATCTTGCCTCCAAAGCTATTTAGTGATGGAGGCCTAAGCCAA
  TCACAAGATGACAGCATTGTGGGAACAAGGCACTGTAGGCATAGTCTGGCTATAATGCCCATCCCTGGAA
  CACTCTCATCCAGCAGCCCTGATCTCCTGCAGCCTACCACCAGTATGTTGGATTTTTCCAATCCTTCAGC
  TGTTGGTTTTTATTATATCCCTGATCAAGTTATAAGAGTTTTCAAAGTGGATCAGCAAAGTTGCTACATT
  ATCATCAGTAAAGACACCACAGCTAAAGAAGTAGTTTTTCATGCTGTTCATGAATTTGGTTTGACCGGTG
  CATCCGACACATATTCTCTCTGTGAAGTTTCTGTTACTCCTGAGGGTGTCATAAAACAGAGAAGACTTCC
  AGATCAGTTCTCCAAATTAGCTGATAGAATTCAACTCAATGGAAGGTATTACTTAAAAAATAACATGGAA
  ACAGAAACCTTATGTTCAGATGAAGATGCTCAAGAACTAGTTAAGGAAAGCCAGCTATCCATGCTGCAGC
  TCAGTACCATTGAGGTGGCCACCCAGCTGTCAATGAGGGACTTTGATTTGTTTCGTAATATTGAACCGAC
  TGAGTACATCGATGACCTTTTTAAGTTAAATTCCAAAACAGGAAATACTCATTTGAAGAGGTTTGAGGAC
  ATTGTAAACCAAGAGACATTCTGGGTTGCCTCAGAAATTTTAACTGAAGCAAATCAGCTCAAACGAATGA
  AGATTATTAAGCATTTTATTAAAATTGCACTTCATTGTCGAGAATGTAAGAACTTCAATTCCATGTTTGC
  AATAATAAGTGGCTTGAACCTGGCATCTGTAGCAAGACTCAGAGGAACTTGGGAAAAGTTACCAAGCAAA
  TACGAGAAACATCTTCAAGATCTACAAGACATTTTTGATCCATCTAGAAACATGGCAAAGTATAGAAATA
  TTCTTAGTAGTCAAAGTATGCAGCCTCCAATTATTCCACTCTTCCCTGTTGTCAAGAAAGATATGACATT
  TCTACATGAAGGAAATGACTCCAAAGTAGATGGTTTAGTAAACTTTGAGAAGTTAAGAATGATTTCCAAG
  GAAATCCGCCAAGTTGTTCGAATGACTTCTGCTAACATGGACCCAGCTATGATGTTTCGACAGAGGAAGA
  AGAGGTGGCGGAGTCTGGGGTCACTGAGTCAAGGAAGCACAAATTCAAACATGCTGGATGTTCAGGGAGG
  TGCTCACAAAAAAAGGGCACGCCGCAGCTCTCTGCTTAATGCCAAGAAGCTATATGAGGATGCCCAAATG
  GCAAGGAAGGTGAAGCAGTATCTTTCCAGTCTCGATGTAGAGACAGATGAGGAGAAGTTCCAGATGATGT
  CATTACAGTGGGAGCCTGCATATGGTACCTTGACCAAGAATTTAAGTGAGAAAAGATCAGCCAAATCATC
  TGAAATGTCTCCAGTGCCTATGAGGTCAGCTGGCCAAACAACTAAAGCCCACTTGCATCAACCCCACAGA
  GTAAGCCAGGTGCTTCAGGTGCCAGCTGTTAATTTGCACCCCATCAGGAAGAAGGGACAAACAAAAGACC
  CTGCACTGAATACAAGTTTACCTCAGAAAGTTTTAGGAACAACTGAAGAAATAAGTGGTAAGAAGCATAC
  AGAAGACACTATTTCTGTGGCGTCATCTTTACATTCTAGTCCTCCTGCATCTCCTCAAGGCTCCCCTCAC
  AAAGGTTACACACTTATTCCATCAGCTAAATCTGACAACTTGTCTGACTCCAGCCATAGTGAGATTTCTT
  CACGGTCCAGCATCGTGAGCAATTGTTCTGTTGACTCCATGTCTGCAGCTCTACAGGATGAACGGTGTTC
  CTCTCAGGCCCTGGCAGTCCCTGAATCCACTGGGGCATTGGAAAAGACAGAGCACGCTTCAGGGATAGGA
  GATCATAGTCAACATGGCCCTGGGTGGACACTCTTGAAGCCATCTCTAATCAAGTGTTTAGCTGTCTCAT
  CGTCTGTGAGCAATGAAGAGATTTCTCAAGAGCATATCATTATAGAAGCAGCTGACAGTGGTCGTGGAAG
  TTGGACTTCGTGTTCAAGCAGCTCCCATGACAACTTCCAAAGCCTTCCAAACCCAAAAAGCTGGGATTTT
  TTGAACTCTTACAGACATACCCATTTGGATGACCCCATTGCTGAAGTTGAACCCACTGACTCTGAGCCCT
  ATTCCTGTTCTAAAAGCTGCTCTAGAACTTGTGGGCAGTGTAAAGGAAGCCTAGAGAGAAAGAGTTGGAC
  CTCCTCCAGTTCTCTGTCTGACACGTATGAACCAAACTATGGGACAGTTAAACAGAGAGTATTGGAGAGC
  ACCCCAGCTGAGTCATCTGAAGGCTTGGACCCCAAGGATGCCACTGACCCAGTTTATAAAACTGTCACTT
  CAAGTACAGAAAAGGGCTTGATTGAAAATGAACAAGTTTCAGCAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228682 representing NM_001164387
  Red=Cloning site Green=Tags(s)
  
  MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETI
  ARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRR
  RFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQA
  TESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPA
  FANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTL
  DKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKAT
  PERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFE
  GDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEG
  VEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVP
  HIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQ
  SQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKVDQQSCYI
  IISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNME
  TETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFED
  IVNQETFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSK
  YEKHLQDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISK
  EIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQM
  ARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHR
  VSQVLQVPAVNLHPIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPH
  KGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIG
  DHSQHGPGWTLLKPSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQSLPNPKSWDF
  LNSYRHTHLDDPIAEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSSSSLSDTYEPNYGTVKQRVLES
  TPAESSEGLDPKDATDPVYKTVTSSTEKGLIENEQVSAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164387
• ORF Size: 4527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164387.2
• RefSeq ORF: 4530 bp
• Locus ID: 51735
• UniProt ID: Q8TEU7
• Cytogenetics: 5q31.1
• MW: 169.3 kDa
• Gene Summary: Guanine nucleotide exchange factor (GEF) for Rap1A, Rap2A and M-Ras GTPases. Does not interact with cAMP.[UniProtKB/Swiss-Prot Function]