RAGEF2 (RAPGEF6) (NM_001164387) Human Untagged Clone

CAT#: SC327317

RAPGEF6 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAGEF2
• Synonyms: KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2; RAGEF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC327317 representing NM_001164387.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAACTCACCCGTGGACCCTGGCGCTAGGCAGGCGTTGAGGAAGAAGCCACCCGAGCGGACTCCCGAG
  GACTTAAATACTATTTATTCTTATCTTCATGGAATGGAAATATTATCAAATCTCAGGGAACATCAGCTT
  AGATTAATGTCTGCAAGAGCACGCTATGAGAGATACAGTGGCAATCAGGTTCTCTTTTGTTCAGAAACG
  ATTGCCAGATGTTGGTATATCCTACTTTCTGGATCTGTGCTTGTGAAAGGCTCCATGGTCTTGCCTCCT
  TGCAGTTTTGGTAAGCAGTTTGGAGGAAAAAGAGGATGTGATTGTCTTGTATTAGAGCCTTCAGAAATG
  ATTGTGGTAGAGAATGCCAAAGATAATGAAGATAGTATTCTACAAAGAGAAATTCCTGCCAGACAATCC
  CGAAGAAGATTTCGGAAAATTAACTATAAAGGAGAGCGCCAAACCATTACTGATGATGTGGAGGTTAAC
  AGCTATCTTTCTCTTCCAGCTGATCTTACCAAGATGCATCTCACAGAAAACCCTCATCCACAGGTGACT
  CATGTGTCTTCTAGTCAGTCTGGTTGTAGCATTGCCAGTGACTCTGGAAGCAGCAGTTTATCTGATATC
  TATCAGGCTACGGAGAGTGAGGTAGGAGATGTAGATTTGACACGTCTTCCAGAAGGACCTGTTGATTCT
  GAGGATGACGAAGAGGAAGATGAAGAGATTGATCGAACAGATCCATTGCAGGGGCGAGATCTTGTTCGA
  GAATGTCTTGAAAAAGAACCTGCAGACAAAACTGATGATGACATTGAACAATTGCTGGAGTTTATGCAC
  CAGCTCCCTGCATTTGCAAACATGACCATGTCTGTAAGGAGAGAACTCTGCTCAGTGATGATTTTTGAA
  GTGGTAGAGCAGGCTGGAGCTATTATTCTTGAAGATGGGCAAGAGCTTGACTCATGGTATGTTATTTTA
  AACGGCACTGTGGAAATCAGTCATCCAGATGGAAAAGTTGAAAATTTGTTTATGGGAAATAGTTTTGGA
  ATTACTCCCACTCTGGATAAGCAGTACATGCATGGAATTGTCAGGACTAAAGTAGATGATTGTCAGTTT
  GTCTGCATAGCCCAGCAAGATTATTGGAGAATTTTAAACCATGTGGAAAAAAATACCCATAAAGTTGAG
  GAAGAGGGAGAAATTGTTATGGTACATGAGCATCGGGAACTAGACCGGAGTGGAACCAGGAAAGGACAC
  ATTGTGATCAAGGCAACACCTGAGCGTCTCATAATGCATTTAATAGAAGAACATTCCATCGTGGATCCA
  ACTTATATAGAAGATTTTCTATTAACTTACAGGACATTTCTTGAAAGTCCTTTGGATGTTGGGATCAAA
  CTATTGGAATGGTTTAAGATCGACAGCTTAAGAGATAAGGTGACACGGATTGTATTATTATGGGTAAAT
  AATCATTTTAATGATTTTGAAGGTGACCCTGCTATGACTCGATTTCTAGAGGAATTTGAAAAAAATCTG
  GAAGATACAAAGATGAATGGTCATCTCCGGTTATTGAATATTGCCTGTGCTGCAAAGGCTAAGTGGAGA
  CAGGTTGTGCTGCAAAAGGCTTCCCGCGAGTCCCCTCTACAATTCAGCCTTAATGGAGGGAGTGAGAAG
  GGATTTGGTATTTTTGTTGAAGGAGTAGAACCTGGTAGCAAAGCTGCTGATTCAGGACTGAAACGTGGT
  GATCAGATTATGGAAGTAAATGGACAAAACTTTGAGAATATTACATTTATGAAAGCCGTTGAAATTTTG
  AGGAATAATACTCATCTTGCACTTACTGTGAAGACCAACATTTTTGTGTTCAAAGAGTTACTTTTTAGG
  ACTGAACAAGAGAAATCTGGTGTTCCTCATATTCCCAAAATTGCTGAAAAAAAAAGTAATCGCCATTCT
  ATCCAGCATGTGCCAGGAGATATTGAACAGACATCACAGGAGAAAGGAAGTAAGAAAGTTAAAGCAAAT
  ACTGTTTCAGGTGGAAGAAACAAAATCAGGAAGATTTTGGATAAAACACGATTTAGTATCTTGCCTCCA
  AAGCTATTTAGTGATGGAGGCCTAAGCCAATCACAAGATGACAGCATTGTGGGAACAAGGCACTGTAGG
  CATAGTCTGGCTATAATGCCCATCCCTGGAACACTCTCATCCAGCAGCCCTGATCTCCTGCAGCCTACC
  ACCAGTATGTTGGATTTTTCCAATCCTTCAGCTGTTGGTTTTTATTATATCCCTGATCAAGTTATAAGA
  GTTTTCAAAGTGGATCAGCAAAGTTGCTACATTATCATCAGTAAAGACACCACAGCTAAAGAAGTAGTT
  TTTCATGCTGTTCATGAATTTGGTTTGACCGGTGCATCCGACACATATTCTCTCTGTGAAGTTTCTGTT
  ACTCCTGAGGGTGTCATAAAACAGAGAAGACTTCCAGATCAGTTCTCCAAATTAGCTGATAGAATTCAA
  CTCAATGGAAGGTATTACTTAAAAAATAACATGGAAACAGAAACCTTATGTTCAGATGAAGATGCTCAA
  GAACTAGTTAAGGAAAGCCAGCTATCCATGCTGCAGCTCAGTACCATTGAGGTGGCCACCCAGCTGTCA
  ATGAGGGACTTTGATTTGTTTCGTAATATTGAACCGACTGAGTACATCGATGACCTTTTTAAGTTAAAT
  TCCAAAACAGGAAATACTCATTTGAAGAGGTTTGAGGACATTGTAAACCAAGAGACATTCTGGGTTGCC
  TCAGAAATTTTAACTGAAGCAAATCAGCTCAAACGAATGAAGATTATTAAGCATTTTATTAAAATTGCA
  CTTCATTGTCGAGAATGTAAGAACTTCAATTCCATGTTTGCAATAATAAGTGGCTTGAACCTGGCATCT
  GTAGCAAGACTCAGAGGAACTTGGGAAAAGTTACCAAGCAAATACGAGAAACATCTTCAAGATCTACAA
  GACATTTTTGATCCATCTAGAAACATGGCAAAGTATAGAAATATTCTTAGTAGTCAAAGTATGCAGCCT
  CCAATTATTCCACTCTTCCCTGTTGTCAAGAAAGATATGACATTTCTACATGAAGGAAATGACTCCAAA
  GTAGATGGTTTAGTAAACTTTGAGAAGTTAAGAATGATTTCCAAGGAAATCCGCCAAGTTGTTCGAATG
  ACTTCTGCTAACATGGACCCAGCTATGATGTTTCGACAGAGGAAGAAGAGGTGGCGGAGTCTGGGGTCA
  CTGAGTCAAGGAAGCACAAATTCAAACATGCTGGATGTTCAGGGAGGTGCTCACAAAAAAAGGGCACGC
  CGCAGCTCTCTGCTTAATGCCAAGAAGCTATATGAGGATGCCCAAATGGCAAGGAAGGTGAAGCAGTAT
  CTTTCCAGTCTCGATGTAGAGACAGATGAGGAGAAGTTCCAGATGATGTCATTACAGTGGGAGCCTGCA
  TATGGTACCTTGACCAAGAATTTAAGTGAGAAAAGATCAGCCAAATCATCTGAAATGTCTCCAGTGCCT
  ATGAGGTCAGCTGGCCAAACAACTAAAGCCCACTTGCATCAACCCCACAGAGTAAGCCAGGTGCTTCAG
  GTGCCAGCTGTTAATTTGCACCCCATCAGGAAGAAGGGACAAACAAAAGACCCTGCACTGAATACAAGT
  TTACCTCAGAAAGTTTTAGGAACAACTGAAGAAATAAGTGGTAAGAAGCATACAGAAGACACTATTTCT
  GTGGCGTCATCTTTACATTCTAGTCCTCCTGCATCTCCTCAAGGCTCCCCTCACAAAGGTTACACACTT
  ATTCCATCAGCTAAATCTGACAACTTGTCTGACTCCAGCCATAGTGAGATTTCTTCACGGTCCAGCATC
  GTGAGCAATTGTTCTGTTGACTCCATGTCTGCAGCTCTACAGGATGAACGGTGTTCCTCTCAGGCCCTG
  GCAGTCCCTGAATCCACTGGGGCATTGGAAAAGACAGAGCACGCTTCAGGGATAGGAGATCATAGTCAA
  CATGGCCCTGGGTGGACACTCTTGAAGCCATCTCTAATCAAGTGTTTAGCTGTCTCATCGTCTGTGAGC
  AATGAAGAGATTTCTCAAGAGCATATCATTATAGAAGCAGCTGACAGTGGTCGTGGAAGTTGGACTTCG
  TGTTCAAGCAGCTCCCATGACAACTTCCAAAGCCTTCCAAACCCAAAAAGCTGGGATTTTTTGAACTCT
  TACAGACATACCCATTTGGATGACCCCATTGCTGAAGTTGAACCCACTGACTCTGAGCCCTATTCCTGT
  TCTAAAAGCTGCTCTAGAACTTGTGGGCAGTGTAAAGGAAGCCTAGAGAGAAAGAGTTGGACCTCCTCC
  AGTTCTCTGTCTGACACGTATGAACCAAACTATGGGACAGTTAAACAGAGAGTATTGGAGAGCACCCCA
  GCTGAGTCATCTGAAGGCTTGGACCCCAAGGATGCCACTGACCCAGTTTATAAAACTGTCACTTCAAGT
  ACAGAAAAGGGCTTGATTGAAAATGAACAAGTTTCAGCAGTCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164387
• Insert Size: 4530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164387.1
• RefSeq Size: 8095 bp
• RefSeq ORF: 4530 bp
• Locus ID: 51735
• UniProt ID: Q8TEU7
• Cytogenetics: 5q31.1
• MW: 169.5 kDa
• Gene Summary: Guanine nucleotide exchange factor (GEF) for Rap1A, Rap2A and M-Ras GTPases. Does not interact with cAMP.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) includes an alternate in-frame exon in the central coding region but lacks another alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 3) is shorter than isoform 1.