SGT1 (ECD) (NM_001135753) Human Tagged ORF Clone
CAT#: RC227341
- TrueORF®
ECD (Myc-DDK-tagged)-Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 3
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001135753" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SGT1 |
Synonyms | GCR2; HSGT1; SGT1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC227341 representing NM_001135753
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAAACCATGAAGCTTGCTACGATGGAAGACACAGTGGAGTACTGCCTGTTCCTGATACCAGATG AGTCAAGGGACTCAGATAAACATAAAGAGATTCTTCAGAAGTACATTGAGAGAATAATCACTCGGTTTGC ACCTATGCTGGTCCCCTACATCTGGCAGAATCAGCCTTTCAATCTTAAATATAAACCTGGGAAAGGAGGT GTTCCTGCTCATATGTTTGGCGTGACAAAGTTTGGGGATAACATTGAGGATGAATGGTTTATTGTTTATG TAATAAAGCAGATCACAAAGGAATTTCCAGAGTTAGTAGCAAGGATTGAAGACAATGATGGTGAATTCTT GTTAATAGAAGCTGCTGACTTTCTCCCTAAATGGCTGGATCCTGAAAATAGCACCAATAGGGTATTTTTC TGCCATGGGGAATTGTGTATTATCCCTGCACCAAGAAAATCTGGAGCAGAATCTTGGTTACCCACCACAC CCCCAACAATTCCACAAGCATTGAATATAATCACAGCACATTCAGAAAAAATACTTGCTTCAGAATCTAT ACGAGCTGCTGTGAATAGGCGCATCAGAGGGTACCCAGAAAAAATTCAGGCCTCACTTCATCGAGCACAC TGCTTCCTTCCAGCTGGCATTGTGGCAGTGCTAAAGCAGCGCCCCAGATTGGTGGCTGCAGCAGTCCAGG CATTTTACCTACGAGACCCTATTGACCTGCGAGCTTGTCGTGTTTTCAAGACATTCTTGCCTGAAACACG AATAATGACATCGGCTCATGGATTTGAGATCTTATGCTCCAAATGTAGCCCACATTTTTCTGACTGCAAG AAATCCCTTGTGACTGCCTCACCACTCTGGGCCAGTTTCCTTGAAAGTCTGAAAAAGAATGATTACTTTA AGGGACTGATAGAAGGTTCTGCTCAGTACCGGGAAAGGCTAGAAATGGCAGAGAATTACTTCCAGCTCTC AGTAGACTGGCCAGAAAGTTCTCTTGCTATGAGCCCTGGTGAAGAAATCTTAACCTTATTACAGACAATA CCATTTGATATAGAAGACCTTAAGAAAGAAGCAGCTAATCTTCCCCCAGAGGATGATGACCAGTGGTTAG ATCTCTCACCAGATCAGCTGGACCAGCTGCTGCAGGAAGCTGTTGGCAAAAAAGAATCCGAGTCTGTTTC CAAGGAGGAGAAGGAGCAGAACTATGACTTAACTGAAGTCTCAGAGAGCATGAAAGCTTTCATATCCAAA GTCTCAACCCACAAGGGAGCAGAGCTGCCTCGAGAACCTTCTGAGGCTCCAATCACTTTTGATGCAGATT CTTTTCTTAATTATTTTGATAAGATTTTAGGGCCAAGGCCTAATGAGTCAGATTCTGATGATCTGGATGA TGAAGACTTTGAATGTTTAGATAGTGATGATGACTTGGACTTTGAAACACACGAACCTGGCGAAGAGGCT TCCCTGAAAGGAACACTTGATAATCTCAAGTCATACATGGCCCAGATGGACCAGGAACTAGCACACACCT GCATCAGCAAAAGTTTCACCACTAGGAACCAAGTGGAACCTGTATCCCAGACTACCGATAACAATTCAGA TGAGGAAGATTCTGGTACGGGAGAATCTGTTATGGCACCAGTAGATGTAGACCTGAACCTGGTTTCAAAT ATATTGGAATCCTATAGCTCCCAAGCTGGACTGGCAGGACCTGCTTCCAATCTTTTACAAAGCATGGGAG TGCAGCTGCCTGACAACACCGATCACAGACCAACAAGTAAGCCAACAAAAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227341 representing NM_001135753
Red=Cloning site Green=Tags(s) MEETMKLATMEDTVEYCLFLIPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPFNLKYKPGKGG VPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIEAADFLPKWLDPENSTNRVFF CHGELCIIPAPRKSGAESWLPTTPPTIPQALNIITAHSEKILASESIRAAVNRRIRGYPEKIQASLHRAH CFLPAGIVAVLKQRPRLVAAAVQAFYLRDPIDLRACRVFKTFLPETRIMTSAHGFEILCSKCSPHFSDCK KSLVTASPLWASFLESLKKNDYFKGLIEGSAQYRERLEMAENYFQLSVDWPESSLAMSPGEEILTLLQTI PFDIEDLKKEAANLPPEDDDQWLDLSPDQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISK VSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEA SLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSN ILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKPTKN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001135753 |
ORF Size | 1803 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001135753.1, NP_001129225.1 |
RefSeq ORF | 1806 bp |
Locus ID | 11319 |
UniProt ID | O95905 |
Cytogenetics | 10q22.2 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 67.5 kDa |
Gene Summary | Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270). May play a role in regulation of pre-mRNA splicing (PubMed:24722212).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227341L3 | Lenti-ORF clone of ECD (Myc-DDK-tagged)-Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 3 |
USD 859.00 |
|
RC227341L4 | Lenti-ORF clone of ECD (mGFP-tagged)-Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 3 |
USD 859.00 |
|
RG227341 | ECD (tGFP-tagged) - Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 3 |
USD 759.00 |
|
SC326098 | ECD (untagged)-Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 3 |
USD 616.00 |
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