SGT1 (ECD) (NM_001135753) Human Untagged Clone

CAT#: SC326098

ECD (untagged)-Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ECD
• Synonyms: GCR2; HSGT1; SGT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC326098 representing NM_001135753.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAAGAAACCATGAAGCTTGCTACGATGGAAGACACAGTGGAGTACTGCCTGTTCCTGATACCAGAT
  GAGTCAAGGGACTCAGATAAACATAAAGAGATTCTTCAGAAGTACATTGAGAGAATAATCACTCGGTTT
  GCACCTATGCTGGTCCCCTACATCTGGCAGAATCAGCCTTTCAATCTTAAATATAAACCTGGGAAAGGA
  GGTGTTCCTGCTCATATGTTTGGCGTGACAAAGTTTGGGGATAACATTGAGGATGAATGGTTTATTGTT
  TATGTAATAAAGCAGATCACAAAGGAATTTCCAGAGTTAGTAGCAAGGATTGAAGACAATGATGGTGAA
  TTCTTGTTAATAGAAGCTGCTGACTTTCTCCCTAAATGGCTGGATCCTGAAAATAGCACCAATAGGGTA
  TTTTTCTGCCATGGGGAATTGTGTATTATCCCTGCACCAAGAAAATCTGGAGCAGAATCTTGGTTACCC
  ACCACACCCCCAACAATTCCACAAGCATTGAATATAATCACAGCACATTCAGAAAAAATACTTGCTTCA
  GAATCTATACGAGCTGCTGTGAATAGGCGCATCAGAGGGTACCCAGAAAAAATTCAGGCCTCACTTCAT
  CGAGCACACTGCTTCCTTCCAGCTGGCATTGTGGCAGTGCTAAAGCAGCGCCCCAGATTGGTGGCTGCA
  GCAGTCCAGGCATTTTACCTACGAGACCCTATTGACCTGCGAGCTTGTCGTGTTTTCAAGACATTCTTG
  CCTGAAACACGAATAATGACATCGGCTCATGGATTTGAGATCTTATGCTCCAAATGTAGCCCACATTTT
  TCTGACTGCAAGAAATCCCTTGTGACTGCCTCACCACTCTGGGCCAGTTTCCTTGAAAGTCTGAAAAAG
  AATGATTACTTTAAGGGACTGATAGAAGGTTCTGCTCAGTACCGGGAAAGGCTAGAAATGGCAGAGAAT
  TACTTCCAGCTCTCAGTAGACTGGCCAGAAAGTTCTCTTGCTATGAGCCCTGGTGAAGAAATCTTAACC
  TTATTACAGACAATACCATTTGATATAGAAGACCTTAAGAAAGAAGCAGCTAATCTTCCCCCAGAGGAT
  GATGACCAGTGGTTAGATCTCTCACCAGATCAGCTGGACCAGCTGCTGCAGGAAGCTGTTGGCAAAAAA
  GAATCCGAGTCTGTTTCCAAGGAGGAGAAGGAGCAGAACTATGACTTAACTGAAGTCTCAGAGAGCATG
  AAAGCTTTCATATCCAAAGTCTCAACCCACAAGGGAGCAGAGCTGCCTCGAGAACCTTCTGAGGCTCCA
  ATCACTTTTGATGCAGATTCTTTTCTTAATTATTTTGATAAGATTTTAGGGCCAAGGCCTAATGAGTCA
  GATTCTGATGATCTGGATGATGAAGACTTTGAATGTTTAGATAGTGATGATGACTTGGACTTTGAAACA
  CACGAACCTGGCGAAGAGGCTTCCCTGAAAGGAACACTTGATAATCTCAAGTCATACATGGCCCAGATG
  GACCAGGAACTAGCACACACCTGCATCAGCAAAAGTTTCACCACTAGGAACCAAGTGGAACCTGTATCC
  CAGACTACCGATAACAATTCAGATGAGGAAGATTCTGGTACGGGAGAATCTGTTATGGCACCAGTAGAT
  GTAGACCTGAACCTGGTTTCAAATATATTGGAATCCTATAGCTCCCAAGCTGGACTGGCAGGACCTGCT
  TCCAATCTTTTACAAAGCATGGGAGTGCAGCTGCCTGACAACACCGATCACAGACCAACAAGTAAGCCA
  ACAAAAAATTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001135753
• Insert Size: 1806 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135753.1
• RefSeq Size: 2151 bp
• RefSeq ORF: 1806 bp
• Locus ID: 11319
• UniProt ID: O95905
• Cytogenetics: 10q22.2
• Protein Families: Druggable Genome, Transcription Factors
• MW: 67.7 kDa
• Gene Summary: Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270). May play a role in regulation of pre-mRNA splicing (PubMed:24722212).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant includes an additional in-frame coding exon compared to variant 1 resulting in a longer protein isoform (3).