ASTE1 (NM_014065) Human Tagged ORF Clone

CAT#: RC218869

ASTE1 (Myc-DDK-tagged)-Human asteroid homolog 1 (Drosophila) (ASTE1)

Product Images

Circular map for RC218869

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  Western blot validation of overexpression lysate (Cat# LY415490) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218869 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ASTE1 protein (Cat# TP318869). The protein was produced from HEK293T cells transfected with ASTE1 cDNA clone (Cat# RC218869) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASTE1
• Synonyms: HT001
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218869 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTATCCGAGGACTAATGAGTTTTGTGGAAGATCATAGTAATGAGTTCTTCACTGATTTGAAGTTGC
  GGGACACAAAAATTGTCATTGATGGTTATGCTCTTTTCCACCGTCTTTGCTTCAGTTCAAACTTGGATCT
  CCGGTATGGAGGGGACTATGATTCTTTTGCAGATGTTGTACAAAAATTCTTTGAATCACTGTTTGCTTGT
  AATATATGCCCATATGTTGTATTAGATGGAGGATGTGACATTTCAGATAAAAAGCTTACAACTTTAAAGG
  ATAGAGCTAGAGAGAAGATCCAGATGGCCCATTCCCTTTCTGTTGGTGGGAGTGGGTACGTATGTCCCTT
  ACTCATCCGGGAAGTATTCATACAGGTTTTGATCAAGCTGCGGGTGTGTTTTGTCCAGTGCTTTTCAGAA
  GCAGATCGGGACATTATGACACTTGCTAACCATTGGAATTGCCCTGTGTTATCATCAGATAGTGACTTTT
  GCATTTTTGACCTGAAAACTGGGTTTTGCCCATTGAATAGCTTTCAGTGGAGAAATATGAACACTATTAA
  GGGCACACAAAACTATATCCCTGCCAAATGCTTTTCCCTTGATGCATTCTGCCATCACTTCAGCAATATG
  AATAAAGCTCTACTACCTCTCTTTGCGGTGCTATGTGGAAATGACCATGTTAATCTACCCATCATGGAGA
  CATTCTTAAGTAAAGCGCGTCTTCCTCTTGGAGCTACCAGTTCTAAAGGGAGGAGACACCACCGAATCCT
  GGGACTTCTGAATTGGTTGTCTCATTTTGCCAACCCTACCGAAGCACTAGATAATGTTCTGAAATACCTC
  CCAAAAAAGGATCGAGAAAATGTTAAGGAACTTCTCTGCTGTTCCATGGAAGAATACCAACAGTCCCAGG
  TGAAGCTACAGGACTTCTTCCAGTGTGGTACTTATGTCTGTCCAGATGCCTTGAATCTTGGTTTACCAGA
  ATGGGTATTAGTGGCTTTAGCTAAAGGCCAGCTATCTCCTTTCATCAGTGATGCTTTGGTCCTAAGACGG
  ACCATTCTTCCCACACAGGTGGAAAACATGCAGCAACCAAATGCCCACAGAATATCTCAGCCCATCAGGC
  AAATCATCTATGGGCTTCTTTTAAATGCCTCACCACATCTGGACAAGACATCCTGGAATGCATTGCCTCC
  TCAGCCTCTAGCTTTCAGTGAAGTGGAAAGGATTAATAAAAATATCAGAACCTCAATCATTGATGCAGTA
  GAACTGGCCAAGGATCATTCTGACTTAAGCAGATTGACTGAGCTCTCCTTGAGGAGGCGGCAGATGCTTC
  TGTTAGAAACCCTGAAGGTGAAACAGACCATTCTGGAGCCAATCCCTACTTCACTGAAGTTGCCCATTGC
  TGTCAGTTGCTACTGGTTGCAGCACACCGAGACCAAAGCAAAGCTACATCATCTACAATCCTTACTGCTC
  ACAATGCTAGTGGGGCCCTTGATTGCCATAATCAACAGCCCTGGTAAGGAAGAGCTGCAGGAAGATGGTG
  CTAAGATGTTGTATGCAGAGTTCCAAAGAGTGAAGGCGCAGACACGGCTGGGCACAAGACTGGACTTAGA
  CACAGCTCACATCTTCTGTCAGTGGCAGTCCTGTCTCCAGATGGGGATGTATCTCAACCAGCTGCTGTCC
  ACTCCTCTCCCAGAGCCAGACCTAACTCGACTGTACAGTGGAAGCCTGGTGCACGGACTATGCCAGCAAC
  TGCTAGCATCGACCTCTGTAGAAAGTCTCCTGAGCATATGTCCTGAGGCTAAGCAACTTTATGAATATCT
  ATTCAATGCCACAAGGTCATATGCCCCCGCTGAAATATTCCTACCAAAAGGTAGATCAAATTCAAAAAAA
  AAAAGGCAGAAGAAACAGAATACCAGCTGTTCTAAGAACAGAGGGAGAACCACTGCACACACCAAGTGTT
  GGTATGAGGGAAACAACCGGTTTGGGTTGTTAATGGTTGAAAACTTAGAGGAACATAGTGAGGCCTCCAA
  CATTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218869 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGIRGLMSFVEDHSNEFFTDLKLRDTKIVIDGYALFHRLCFSSNLDLRYGGDYDSFADVVQKFFESLFAC
  NICPYVVLDGGCDISDKKLTTLKDRAREKIQMAHSLSVGGSGYVCPLLIREVFIQVLIKLRVCFVQCFSE
  ADRDIMTLANHWNCPVLSSDSDFCIFDLKTGFCPLNSFQWRNMNTIKGTQNYIPAKCFSLDAFCHHFSNM
  NKALLPLFAVLCGNDHVNLPIMETFLSKARLPLGATSSKGRRHHRILGLLNWLSHFANPTEALDNVLKYL
  PKKDRENVKELLCCSMEEYQQSQVKLQDFFQCGTYVCPDALNLGLPEWVLVALAKGQLSPFISDALVLRR
  TILPTQVENMQQPNAHRISQPIRQIIYGLLLNASPHLDKTSWNALPPQPLAFSEVERINKNIRTSIIDAV
  ELAKDHSDLSRLTELSLRRRQMLLLETLKVKQTILEPIPTSLKLPIAVSCYWLQHTETKAKLHHLQSLLL
  TMLVGPLIAIINSPGKEELQEDGAKMLYAEFQRVKAQTRLGTRLDLDTAHIFCQWQSCLQMGMYLNQLLS
  TPLPEPDLTRLYSGSLVHGLCQQLLASTSVESLLSICPEAKQLYEYLFNATRSYAPAEIFLPKGRSNSKK
  KRQKKQNTSCSKNRGRTTAHTKCWYEGNNRFGLLMVENLEEHSEASNIE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014065
• ORF Size: 2037 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014065.4
• RefSeq Size: 2736 bp
• RefSeq ORF: 2040 bp
• Locus ID: 28990
• UniProt ID: Q2TB18
• Cytogenetics: 3q22.1
• MW: 77.1 kDa
• Gene Summary: Possible role in EGF receptor signaling.[UniProtKB/Swiss-Prot Function]