ASTE1 (NM_014065) Human Tagged ORF Clone
CAT#: RC218869
ASTE1 (Myc-DDK-tagged)-Human asteroid homolog 1 (Drosophila) (ASTE1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_014065" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ASTE1 |
Synonyms | HT001 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC218869 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTATCCGAGGACTAATGAGTTTTGTGGAAGATCATAGTAATGAGTTCTTCACTGATTTGAAGTTGC GGGACACAAAAATTGTCATTGATGGTTATGCTCTTTTCCACCGTCTTTGCTTCAGTTCAAACTTGGATCT CCGGTATGGAGGGGACTATGATTCTTTTGCAGATGTTGTACAAAAATTCTTTGAATCACTGTTTGCTTGT AATATATGCCCATATGTTGTATTAGATGGAGGATGTGACATTTCAGATAAAAAGCTTACAACTTTAAAGG ATAGAGCTAGAGAGAAGATCCAGATGGCCCATTCCCTTTCTGTTGGTGGGAGTGGGTACGTATGTCCCTT ACTCATCCGGGAAGTATTCATACAGGTTTTGATCAAGCTGCGGGTGTGTTTTGTCCAGTGCTTTTCAGAA GCAGATCGGGACATTATGACACTTGCTAACCATTGGAATTGCCCTGTGTTATCATCAGATAGTGACTTTT GCATTTTTGACCTGAAAACTGGGTTTTGCCCATTGAATAGCTTTCAGTGGAGAAATATGAACACTATTAA GGGCACACAAAACTATATCCCTGCCAAATGCTTTTCCCTTGATGCATTCTGCCATCACTTCAGCAATATG AATAAAGCTCTACTACCTCTCTTTGCGGTGCTATGTGGAAATGACCATGTTAATCTACCCATCATGGAGA CATTCTTAAGTAAAGCGCGTCTTCCTCTTGGAGCTACCAGTTCTAAAGGGAGGAGACACCACCGAATCCT GGGACTTCTGAATTGGTTGTCTCATTTTGCCAACCCTACCGAAGCACTAGATAATGTTCTGAAATACCTC CCAAAAAAGGATCGAGAAAATGTTAAGGAACTTCTCTGCTGTTCCATGGAAGAATACCAACAGTCCCAGG TGAAGCTACAGGACTTCTTCCAGTGTGGTACTTATGTCTGTCCAGATGCCTTGAATCTTGGTTTACCAGA ATGGGTATTAGTGGCTTTAGCTAAAGGCCAGCTATCTCCTTTCATCAGTGATGCTTTGGTCCTAAGACGG ACCATTCTTCCCACACAGGTGGAAAACATGCAGCAACCAAATGCCCACAGAATATCTCAGCCCATCAGGC AAATCATCTATGGGCTTCTTTTAAATGCCTCACCACATCTGGACAAGACATCCTGGAATGCATTGCCTCC TCAGCCTCTAGCTTTCAGTGAAGTGGAAAGGATTAATAAAAATATCAGAACCTCAATCATTGATGCAGTA GAACTGGCCAAGGATCATTCTGACTTAAGCAGATTGACTGAGCTCTCCTTGAGGAGGCGGCAGATGCTTC TGTTAGAAACCCTGAAGGTGAAACAGACCATTCTGGAGCCAATCCCTACTTCACTGAAGTTGCCCATTGC TGTCAGTTGCTACTGGTTGCAGCACACCGAGACCAAAGCAAAGCTACATCATCTACAATCCTTACTGCTC ACAATGCTAGTGGGGCCCTTGATTGCCATAATCAACAGCCCTGGTAAGGAAGAGCTGCAGGAAGATGGTG CTAAGATGTTGTATGCAGAGTTCCAAAGAGTGAAGGCGCAGACACGGCTGGGCACAAGACTGGACTTAGA CACAGCTCACATCTTCTGTCAGTGGCAGTCCTGTCTCCAGATGGGGATGTATCTCAACCAGCTGCTGTCC ACTCCTCTCCCAGAGCCAGACCTAACTCGACTGTACAGTGGAAGCCTGGTGCACGGACTATGCCAGCAAC TGCTAGCATCGACCTCTGTAGAAAGTCTCCTGAGCATATGTCCTGAGGCTAAGCAACTTTATGAATATCT ATTCAATGCCACAAGGTCATATGCCCCCGCTGAAATATTCCTACCAAAAGGTAGATCAAATTCAAAAAAA AAAAGGCAGAAGAAACAGAATACCAGCTGTTCTAAGAACAGAGGGAGAACCACTGCACACACCAAGTGTT GGTATGAGGGAAACAACCGGTTTGGGTTGTTAATGGTTGAAAACTTAGAGGAACATAGTGAGGCCTCCAA CATTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218869 protein sequence
Red=Cloning site Green=Tags(s) MGIRGLMSFVEDHSNEFFTDLKLRDTKIVIDGYALFHRLCFSSNLDLRYGGDYDSFADVVQKFFESLFAC NICPYVVLDGGCDISDKKLTTLKDRAREKIQMAHSLSVGGSGYVCPLLIREVFIQVLIKLRVCFVQCFSE ADRDIMTLANHWNCPVLSSDSDFCIFDLKTGFCPLNSFQWRNMNTIKGTQNYIPAKCFSLDAFCHHFSNM NKALLPLFAVLCGNDHVNLPIMETFLSKARLPLGATSSKGRRHHRILGLLNWLSHFANPTEALDNVLKYL PKKDRENVKELLCCSMEEYQQSQVKLQDFFQCGTYVCPDALNLGLPEWVLVALAKGQLSPFISDALVLRR TILPTQVENMQQPNAHRISQPIRQIIYGLLLNASPHLDKTSWNALPPQPLAFSEVERINKNIRTSIIDAV ELAKDHSDLSRLTELSLRRRQMLLLETLKVKQTILEPIPTSLKLPIAVSCYWLQHTETKAKLHHLQSLLL TMLVGPLIAIINSPGKEELQEDGAKMLYAEFQRVKAQTRLGTRLDLDTAHIFCQWQSCLQMGMYLNQLLS TPLPEPDLTRLYSGSLVHGLCQQLLASTSVESLLSICPEAKQLYEYLFNATRSYAPAEIFLPKGRSNSKK KRQKKQNTSCSKNRGRTTAHTKCWYEGNNRFGLLMVENLEEHSEASNIE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014065 |
ORF Size | 2037 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014065.4 |
RefSeq Size | 2736 bp |
RefSeq ORF | 2040 bp |
Locus ID | 28990 |
UniProt ID | Q2TB18 |
Cytogenetics | 3q22.1 |
MW | 77.1 kDa |
Gene Summary | Possible role in EGF receptor signaling.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218869L3 | Lenti ORF clone of Human asteroid homolog 1 (Drosophila) (ASTE1), Myc-DDK-tagged |
USD 922.00 |
|
RC218869L4 | Lenti ORF clone of Human asteroid homolog 1 (Drosophila) (ASTE1), mGFP tagged |
USD 922.00 |
|
RG218869 | ASTE1 (tGFP-tagged) - Human asteroid homolog 1 (Drosophila) (ASTE1) |
USD 822.00 |
|
SC115202 | ASTE1 (untagged)-Human asteroid homolog 1 (Drosophila) (ASTE1) |
USD 835.00 |
|
SC318150 | ASTE1 (untagged)-Human asteroid homolog 1 (Drosophila) (ASTE1) |
USD 685.00 |
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