ASTE1 (NM_014065) Human Untagged Clone

CAT#: SC318150

ASTE1 (untagged)-Human asteroid homolog 1 (Drosophila) (ASTE1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASTE1
• Synonyms: HT001
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318150 representing NM_014065.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGTATCCGAGGACTAATGAGTTTTGTGGAAGATCATAGTAATGAGTTCTTCACTGATTTGAAGTTG
  CGGGACACAAAAATTGTCATTGATGGTTATGCTCTTTTCCACCGTCTTTGCTTCAGTTCAAACTTGGAT
  CTCCGGTATGGAGGGGACTATGATTCTTTTGCAGATGTTGTACAAAAATTCTTTGAATCACTGTTTGCT
  TGTAATATATGCCCATATGTTGTATTAGATGGAGGATGTGACATTTCAGATAAAAAGCTTACAACTTTA
  AAGGATAGAGCTAGAGAGAAGATCCAGATGGCCCATTCCCTTTCTGTTGGTGGGAGTGGGTACGTATGT
  CCCTTACTCATCCGGGAAGTATTCATACAGGTTTTGATCAAGCTGCGGGTGTGTTTTGTCCAGTGCTTT
  TCAGAAGCAGATCGGGACATTATGACACTTGCTAACCATTGGAATTGCCCTGTGTTATCATCAGATAGT
  GACTTTTGCATTTTTGACCTGAAAACTGGGTTTTGCCCATTGAATAGCTTTCAGTGGAGAAATATGAAC
  ACTATTAAGGGCACACAAAACTATATCCCTGCCAAATGCTTTTCCCTTGATGCATTCTGCCATCACTTC
  AGCAATATGAATAAAGCTCTACTACCTCTCTTTGCGGTGCTATGTGGAAATGACCATGTTAATCTACCC
  ATCATGGAGACATTCTTAAGTAAAGCGCGTCTTCCTCTTGGAGCTACCAGTTCTAAAGGGAGGAGACAC
  CACCGAATCCTGGGACTTCTGAATTGGTTGTCTCATTTTGCCAACCCTACCGAAGCACTAGATAATGTT
  CTGAAATACCTCCCAAAAAAGGATCGAGAAAATGTTAAGGAACTTCTCTGCTGTTCCATGGAAGAATAC
  CAACAGTCCCAGGTGAAGCTACAGGACTTCTTCCAGTGTGGTACTTATGTCTGTCCAGATGCCTTGAAT
  CTTGGTTTACCAGAATGGGTATTAGTGGCTTTAGCTAAAGGCCAGCTATCTCCTTTCATCAGTGATGCT
  TTGGTCCTAAGACGGACCATTCTTCCCACACAGGTGGAAAACATGCAGCAACCAAATGCCCACAGAATA
  TCTCAGCCCATCAGGCAAATCATCTATGGGCTTCTTTTAAATGCCTCACCACATCTGGACAAGACATCC
  TGGAATGCATTGCCTCCTCAGCCTCTAGCTTTCAGTGAAGTGGAAAGGATTAATAAAAATATCAGAACC
  TCAATCATTGATGCAGTAGAACTGGCCAAGGATCATTCTGACTTAAGCAGATTGACTGAGCTCTCCTTG
  AGGAGGCGGCAGATGCTTCTGTTAGAAACCCTGAAGGTGAAACAGACCATTCTGGAGCCAATCCCTACT
  TCACTGAAGTTGCCCATTGCTGTCAGTTGCTACTGGTTGCAGCACACCGAGACCAAAGCAAAGCTACAT
  CATCTACAATCCTTACTGCTCACAATGCTAGTGGGGCCCTTGATTGCCATAATCAACAGCCCTGGTAAG
  GAAGAGCTGCAGGAAGATGGTGCTAAGATGTTGTATGCAGAGTTCCAAAGAGTGAAGGCGCAGACACGG
  CTGGGCACAAGACTGGACTTAGACACAGCTCACATCTTCTGTCAGTGGCAGTCCTGTCTCCAGATGGGG
  ATGTATCTCAACCAGCTGCTGTCCACTCCTCTCCCAGAGCCAGACCTAACTCGACTGTACAGTGGAAGC
  CTGGTGCACGGACTATGCCAGCAACTGCTAGCATCGACCTCTGTAGAAAGTCTCCTGAGCATATGTCCT
  GAGGCTAAGCAACTTTATGAATATCTATTCAATGCCACAAGGTCATATGCCCCCGCTGAAATATTCCTA
  CCAAAAGGTAGATCAAATTCAAAAAAAAAAAGGCAGAAGAAACAGAATACCAGCTGTTCTAAGAACAGA
  GGGAGAACCACTGCACACACCAAGTGTTGGTATGAGGGAAACAACCGGTTTGGGTTGTTAATGGTTGAA
  AACTTAGAGGAACATAGTGAGGCCTCCAACATTGAATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014065
• Insert Size: 2040 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014065.3
• RefSeq Size: 2736 bp
• RefSeq ORF: 2040 bp
• Locus ID: 28990
• UniProt ID: Q2TB18
• Cytogenetics: 3q22.1
• MW: 77.1 kDa
• Gene Summary: Possible role in EGF receptor signaling.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.