E2 (NC_014185) Virus Tagged ORF Clone

CAT#: VC101799

Myc-DDK-tagged ORF clone of viral ORF for regulatory protein E2 [Human papillomavirus 121], codon optimized for human cell expression, YP_003668028

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101799 represents NCBI reference of YP_003668028 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGCTGCGCGAACGCTTCGATGCTGTGCAAGAGGCGCTCCTGACACTTTATGAAAACGCCGCCA
  AAGACATTGATACCCAGATCCAGCATTGGGAGCTGCTCAGACAGGAGCACGTCATCATGTACTATGCTAG
  ACAGAACGGAATAAAGCGCATCGGATTCCACCAGGTGCCAAGCTTGGCAACATCCGAACACAAAGCCAAG
  GAGGCCATCCGGATGACAATCCTGCTCAAATCTTTGAAAGACTCTCCTTTCGGGTCTGAGACATGGAGTC
  TTACGGAAACATCCCTGGAAATTGTCAACGCCGCACCGAGTAACTGCTTTAAGAAGGAAGCATATATCGT
  GGATGTGTATTATGATCGCGATGCGGATAAAGTTTATCCATACACAGGGTGGAAGCATATTTATTTTCAG
  GGCGAAGACAATATGTGGCACAAAACCCCTGGCCGCGTGGACTACTATGGTCTGTACTACATCGATGACT
  ATGGTGACAAAGTCTATTATGTGAACTTCGACGAGAAAGCCCAGACGCTTTCCGTCACAGGAGAGTGGGA
  GGTAAAATACCAAAATAAGACTATCTCTCCCTCCGTTACTTCTAGCTCTAGCCCCACCGCCTGGAAACCC
  GCTCAGGGCCACACTTCTCACTCCCCCTGGAATAGTGAAACAATAGAGGAGGTCAGAAGAAAACAAGAAG
  GCCCAAAGAACACCTCTGTTCCCTCCTCCGAAGAGACACCCGGACTGCGGCATAACAGAAGAAGACGAGA
  GGGTGAGAGCGGAATCACCAGGTCTCCCAAGCGGCGGCGGACCGATATTCCAGACAGCACCGGCCCTGCG
  TACCCAACTCCAGCCCAGGTAGGTAGTAGCCATCGGTCCGCGTCCGGGCACTATAGTTCCCGGCTTGAAC
  GACTGCAGGCGGAAGCCAGAGACCCGCCCATAATTATTGTTAAGGGATGCGCTAACACACTTAAATGCTG
  GAGGTTCCGGTTCAAGAAACAGAATAGAAAACTGTTCGAGCAGGCGTCAACCGTGTTTAAATGGGTGGAT
  TATGACAGTTCCAGCCGGATGCTGCTTTCATTCAAGGATACAGCACAGAGACAGCTGTTTCTCGACACCG
  TTACTTTGCCTAAAGGAACCCAGGTTGCTCTGGGGAGTCTTAACAGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101799 representing YP_003668028
  Red=Cloning sites Green=Tags
  
  MEKLRERFDAVQEALLTLYENAAKDIDTQIQHWELLRQEHVIMYYARQNGIKRIGFHQVPSLATSEHKAK
  EAIRMTILLKSLKDSPFGSETWSLTETSLEIVNAAPSNCFKKEAYIVDVYYDRDADKVYPYTGWKHIYFQ
  GEDNMWHKTPGRVDYYGLYYIDDYGDKVYYVNFDEKAQTLSVTGEWEVKYQNKTISPSVTSSSSPTAWKP
  AQGHTSHSPWNSETIEEVRRKQEGPKNTSVPSSEETPGLRHNRRRREGESGITRSPKRRRTDIPDSTGPA
  YPTPAQVGSSHRSASGHYSSRLERLQAEARDPPIIIVKGCANTLKCWRFRFKKQNRKLFEQASTVFKWVD
  YDSSSRMLLSFKDTAQRQLFLDTVTLPKGTQVALGSLNSL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014185
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_014185.1, YP_003668028
• RefSeq ORF: 1170 bp
• Locus ID: 9231101
• MW: 44.9 kDa