E6 (NC_014185) Virus Tagged ORF Clone

CAT#: VC101796

Myc-DDK-tagged ORF clone of viral ORF for transforming protein E6 [Human papillomavirus 121], codon optimized for human cell expression, YP_003668025

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101796 represents NCBI reference of YP_003668025 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTCCAACAGAGCCCAACACCTGTTACAATTACCGAATACTGCAATACATTCGGTTTTTCCCTCA
  AAGATATCAGGCTCCCCTGCACCTTTTGCCGGTTTTATCTGACCATACAAGACTTGGCCTCCTTCGAGCT
  GAAGCAGTTCAGGCTGCTCTGGAAGGGACCCTTTTGTTATGCATGCTGTAGATCTTGTATGAGGCTTACC
  GCAGCCTTTGAGGTCCGGAAGCACTATCAGTGTTCATGCAGCTGCCTGTTTCTTGAAGATCTTCTCGGTC
  GACCACTCCAGCACATCCCAATGAGATGCCTTCTGTGCCTCGCCCTTCTTGACTATGCTGAAAAACTCCA
  GCATTTCTGCGTGAGAGAAGACTTTGTGCTGGTTCGAGGCAACTGGAGGGGATACTGCCGAAACTGTATC
  AGAAAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101796 representing YP_003668025
  Red=Cloning sites Green=Tags
  
  MDLQQSPTPVTITEYCNTFGFSLKDIRLPCTFCRFYLTIQDLASFELKQFRLLWKGPFCYACCRSCMRLT
  AAFEVRKHYQCSCSCLFLEDLLGRPLQHIPMRCLLCLALLDYAEKLQHFCVREDFVLVRGNWRGYCRNCI
  RKE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014185
• ORF Size: 429 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NC_014185.1, YP_003668025
• RefSeq ORF: 429 bp
• Locus ID: 9231105
• MW: 16.9 kDa