SFI1 (NM_001258326) Human Untagged Clone

CAT#: SC332661

SFI1 (untagged) - Homo sapiens Sfi1 homolog, spindle assembly associated (yeast) (SFI1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SFI1
• Synonyms: hSfi1p; PISD; PPP1R139
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332661 representing NM_001258326.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGAAGAATCTGCTCACTGAGAAGTGTATATCAAGCCACAATTTCCATCAAAAAGTGATTAAGCAGAGA
  ATGGAGAAGAAGGTTGATTCCAGATTTTACTATGAGCAGCGATTACTACGGAAGGTCTTCGAAGAATGG
  AAAGAGGAGTGGTGGGTTTTCCAGCACGAGTGGAAACTCTGTGTTCGAGCTGACTGTCACTACAGGTAT
  TACCTGTACAACCTGATGTTCCAGACGTGGAAGACCTATGTGCGTCAGCAGCAGGAGATGAGGAACAAG
  TACATTAGAGCCGAGGTTCATGATGCAAAGCAAAAGATGCGACAGGCCTGGAAGTCCTGGTTGATCTAC
  GTGGTTGTTCGTAGGACCAAACTTCAGATGCAGACTACAGCTCTGGAGTTTAGGCAACGGATTATCTTA
  CGGGTGTGGTGGAGCACGTGGAGGCAGCGACTAGGACAGGTCCGTGTGAGCCGTGCCCTCCATGCCTCT
  GCTTTGAAGCACAGGGCCCTGAGCCTCCAGGTGCAGGCTTGGTCACAGTGGCGGGAACAGCTCCTGTAT
  GTCCAGAAGGAGAAACAAAAGGTTGTCTCTGCAGTGAAACATCATCAGCACTGGCAAAAACGGAGATTT
  CTAAAGGCCTGGCTTGAATACCTGCAAGTCCGCAGAGTGAAGAGACAGCAGAATGAGATGGCTGAGCGA
  TTCCATCATGTCACTGTGCTCCAGATATACTTCTGTGACTGGCAGCAGGCCTGGGAGCGGAGGGAGAGC
  TTGTACGCTCACCATGCCCAGGTGGAGAAACTGGCCAGGAAGATGGCCCTGCGGCGCGCCTTTACTCAC
  TGGAAACACTACATGCTGCTGTGTGCAGAAGAAGCTGCCCAGTTTGAGATGGCAGAAGAGCACCACAGG
  CACAGCCAGCTGTATTTTTGCTTTAGAGCCCTAAAAGACAATGTGACCCACGCTCATCTCCAGCAAATA
  AGAAGGAATCTTGCTCACCAGCAGCATGGTGTCACGCTGCTGCACAGGTTCTGGAACCTCTGGCGGTCT
  CAGATTGAGCAGAAAAAGGAAAGAGAGCTGCTCCCCTTACTGCATGCTGCCTGGGACCACTACAGAATA
  GCACTGCTGTGCAAATGTATCGAATTGTGGCTACAGTATACTCAGAAGAGGCGGTACAAGCAGCTGCTA
  CAGGCCAGAGCGGATGGTCATTTCCAGCAGAGAGCCCTGCCTGCTGCCTTCCACACATGGAACAGACTC
  TGGCGATGGCGCCACCAGGAAAATGTCCTCAGTGCAAGAGCAACACGTTTCCACAGGGAGACATTAGAG
  AAGCAAGTATTTTCTCTCTGGAGGCAGAAGATGTTTCAGCATCGAGAAAACCGCCTGGCAGAGAGAATG
  GCCATCCTTCACGCAGAGCGACAGCTTCTGTATAGGTCTTGGTTCATGTGGCACCAGCAGGCAGCAGCA
  CGTCACCAGGAGCAGGAGTGGCAAACAGTGGCCTGTGCCCACCACCGCCACGGGCGGCTCAAGAAAGCT
  TTCTGCCTCTGGAGGGAAAGTGCCCAAGGGCTCAGAACAGAGAGGACGGGCAGGGTGCGGGCAGCAGAA
  TTCCACATGGCCCAGCTCCTGCGTTGGGCCTGGAGCCAGTGGAGGGAGTGCCTGGCCCTGCGGGGAGCG
  GAGCGGCAGAAGCTGATGCGAGCAGACCTGCACCACCAGCACAGCGTGCTGCACAGGGCGCTGCAGGCA
  TGGGTGACTTACCAGGGCAGGGTGCGAAGCATCCTCCGGGAGGTGGCAGCCAGGGAGAGCCAGCACAAC
  AGGCAGCTGCTGCGCGGGGCATTACGTCGCTGGAAAGAGAACACCATGGCCCGAGTGGATGAAGCCAAA
  AAAACCTTTCAAGCAAGTACTCATTACAGAAGGACCATATGTTCCAAGGTCCTGGTCCAGTGGCGGGAA
  GCTGTGTCAGTGCAGATGTATTACCGACAGCAGGAGGACTGTGCCATCTGGGAGGCCCAGAAGGTGCTG
  GACAGGGGCTGTCTGCGGACCTGGTTTCAGCGCTGGTGGGACTGCAGCCGGAGGTCAGCCCAGCAGAGA
  CTGCAGCTGGAGAGGGCAGTGCAACACCACCACCGGCAGCTGCTGCTGGAGGGGCTGGCCCGGTGGAAG
  ACGCACCATCTGCAGTGTGTCAGGAAGAGGCTCCTGCACAGGCAGAGCACCCAACTGCTGGCACAGAGA
  CTCAGCCGGACCTGCTTCCGCCAGTGGAGACAACAGCTGGCAGCCAGGAGGCAGGAGCAGCGGGCGACA
  GTGCGGGCCCTGTGGTTCTGGGCCTTCTCGCTGCAGGCAAAGGTGTGGGCCACGTGGCTGGCCTTTGTA
  CTGGAAAGGAGGAGAAAGAAGGCGCGGCTGCAGTGGGCGCTCCAGGCCTACCAGGGGCAGCTCCTCCAG
  GAGGGTGCCACGCGGCTCCTGCGCTTTGCAGCCAGCATGAAGGCCTCCCGGCAGCAGCTGCAGGCCCAG
  CAGCAGGTCCAGGCGGCTCACAGTCTCCATCGTGCCGTCCGCCGCTGTGCCACGCTCTGGAAACAGAAA
  GTGCTGGGCCGGGGCGGGAAGCCTCAGCCCCTGGCAGCCATCGCACCCAGCAGGAAAGTGACGTTTGAG
  GGTCCCCTTCTCAACCGCATTGCTGCTGGGGCTGGGGATGGCACCCTTGAGACCAAGAGGCCACAGGCT
  TCTCGGCCTCTGGGAGCTCTGGGCCGCCTGGCTGCTGAGGAGCCCCACGCCCTGGAGCTCAACACTGCC
  CACTCAGCGAGGAAGCAGCCGCGACGCCCACACTTCCTGTTGGAGCCTGCGCAGAGCCAGAGGCCTCAG
  AAGCCACAGGAACATGGCCTAGGCATGGCTCAGCCAGCAGCCCCCTCCCTGACGCGGCCCTTCCTGGCA
  GAGGCCCCGACAGCACTGGTCCCACACAGCCCCCTGCCTGGGGCCCTGTCAAGCGCCCCTGGCCCGAAG
  CAGCCCCCGACGGCAAGCACAGGCCCGGAGCTGCTGCTGCTGCCTCTTTCCTCCTTCATGCCCTGCGGG
  GCGGCTGCACCAGCCAGGGTGTCAGCACAGCGGGCTACTCCTAGGGATAAGCCCCCGGTCCCCTCATCC
  CTGGCCAGTGTCCCTGACCCCCATCTACTCCTTCCTGGGGACTTCTCAGCCACCAGGGCTGGGCCTGGA
  CTTTCAACTGCAGGCAGCCTGGACCTTGAGGCTGAACTTGAGGAGATCCAGCAGCAACTACTGCACTAC
  CAGACCACCAAGCAGAACCTCTGGTCCTGTCGGCGGCAAGCGAGCAGCCTGCGCAGGTGGCTGGAGCTG
  AACAGAGAGGAGCCGGGGCCTGAGGACCAGGAAGTAGAGCAGCAGGTGCAGAAAGAGCTGGAACAGGTG
  GAAATGCAGATCCAGCTGCTGGCAGAGGAGCTCCAGGCTCAGCGCCAGCCCATTGGCGCCTGCGTTGCC
  CGCATCCAGGCCCTGCGGCAGGCCCTGTGCTAG
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001258326
• Insert Size: 3483 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258326.1
• RefSeq Size: 4141 bp
• RefSeq ORF: 3483 bp
• Locus ID: 9814
• UniProt ID: A8K8P3
• Cytogenetics: 22q12.2
• MW: 138.1 kDa
• Gene Summary: Plays a role in the dynamic structure of centrosome-associated contractile fibers via its interaction with CETN2.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) uses an alternate splice site in the 5' UTR and lacks two consecutive exons in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (d) is shorter than isoform a.