SFI1 (NM_001258326) Human Tagged ORF Clone

CAT#: RG235199

SFI1 (tGFP-tagged) - Homo sapiens Sfi1 homolog, spindle assembly associated (yeast) (SFI1), transcript variant 4

Product Images

Circular map for RG235199

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SFI1
• Synonyms: hSfi1p; PISD; PPP1R139
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235199 representing NM_001258326
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAATCTGCTCACTGAGAAGTGTATATCAAGCCACAATTTCCATCAAAAAGTGATTAAGCAGAGAA
  TGGAGAAGAAGGTTGATTCCAGATTTTACTATGAGCAGCGATTACTACGGAAGGTCTTCGAAGAATGGAA
  AGAGGAGTGGTGGGTTTTCCAGCACGAGTGGAAACTCTGTGTTCGAGCTGACTGTCACTACAGGTATTAC
  CTGTACAACCTGATGTTCCAGACGTGGAAGACCTATGTGCGTCAGCAGCAGGAGATGAGGAACAAGTACA
  TTAGAGCCGAGGTTCATGATGCAAAGCAAAAGATGCGACAGGCCTGGAAGTCCTGGTTGATCTACGTGGT
  TGTTCGTAGGACCAAACTTCAGATGCAGACTACAGCTCTGGAGTTTAGGCAACGGATTATCTTACGGGTG
  TGGTGGAGCACGTGGAGGCAGCGACTAGGACAGGTCCGTGTGAGCCGTGCCCTCCATGCCTCTGCTTTGA
  AGCACAGGGCCCTGAGCCTCCAGGTGCAGGCTTGGTCACAGTGGCGGGAACAGCTCCTGTATGTCCAGAA
  GGAGAAACAAAAGGTTGTCTCTGCAGTGAAACATCATCAGCACTGGCAAAAACGGAGATTTCTAAAGGCC
  TGGCTTGAATACCTGCAAGTCCGCAGAGTGAAGAGACAGCAGAATGAGATGGCTGAGCGATTCCATCATG
  TCACTGTGCTCCAGATATACTTCTGTGACTGGCAGCAGGCCTGGGAGCGGAGGGAGAGCTTGTACGCTCA
  CCATGCCCAGGTGGAGAAACTGGCCAGGAAGATGGCCCTGCGGCGCGCCTTTACTCACTGGAAACACTAC
  ATGCTGCTGTGTGCAGAAGAAGCTGCCCAGTTTGAGATGGCAGAAGAGCACCACAGGCACAGCCAGCTGT
  ATTTTTGCTTTAGAGCCCTAAAAGACAATGTGACCCACGCTCATCTCCAGCAAATAAGAAGGAATCTTGC
  TCACCAGCAGCATGGTGTCACGCTGCTGCACAGGTTCTGGAACCTCTGGCGGTCTCAGATTGAGCAGAAA
  AAGGAAAGAGAGCTGCTCCCCTTACTGCATGCTGCCTGGGACCACTACAGAATAGCACTGCTGTGCAAAT
  GTATCGAATTGTGGCTACAGTATACTCAGAAGAGGCGGTACAAGCAGCTGCTACAGGCCAGAGCGGATGG
  TCATTTCCAGCAGAGAGCCCTGCCTGCTGCCTTCCACACATGGAACAGACTCTGGCGATGGCGCCACCAG
  GAAAATGTCCTCAGTGCAAGAGCAACACGTTTCCACAGGGAGACATTAGAGAAGCAAGTATTTTCTCTCT
  GGAGGCAGAAGATGTTTCAGCATCGAGAAAACCGCCTGGCAGAGAGAATGGCCATCCTTCACGCAGAGCG
  ACAGCTTCTGTATAGGTCTTGGTTCATGTGGCACCAGCAGGCAGCAGCACGTCACCAGGAGCAGGAGTGG
  CAAACAGTGGCCTGTGCCCACCACCGCCACGGGCGGCTCAAGAAAGCTTTCTGCCTCTGGAGGGAAAGTG
  CCCAAGGGCTCAGAACAGAGAGGACGGGCAGGGTGCGGGCAGCAGAATTCCACATGGCCCAGCTCCTGCG
  TTGGGCCTGGAGCCAGTGGAGGGAGTGCCTGGCCCTGCGGGGAGCGGAGCGGCAGAAGCTGATGCGAGCA
  GACCTGCACCACCAGCACAGCGTGCTGCACAGGGCGCTGCAGGCATGGGTGACTTACCAGGGCAGGGTGC
  GAAGCATCCTCCGGGAGGTGGCAGCCAGGGAGAGCCAGCACAACAGGCAGCTGCTGCGCGGGGCATTACG
  TCGCTGGAAAGAGAACACCATGGCCCGAGTGGATGAAGCCAAAAAAACCTTTCAAGCAAGTACTCATTAC
  AGAAGGACCATATGTTCCAAGGTCCTGGTCCAGTGGCGGGAAGCTGTGTCAGTGCAGATGTATTACCGAC
  AGCAGGAGGACTGTGCCATCTGGGAGGCCCAGAAGGTGCTGGACAGGGGCTGTCTGCGGACCTGGTTTCA
  GCGCTGGTGGGACTGCAGCCGGAGGTCAGCCCAGCAGAGACTGCAGCTGGAGAGGGCAGTGCAACACCAC
  CACCGGCAGCTGCTGCTGGAGGGGCTGGCCCGGTGGAAGACGCACCATCTGCAGTGTGTCAGGAAGAGGC
  TCCTGCACAGGCAGAGCACCCAACTGCTGGCACAGAGACTCAGCCGGACCTGCTTCCGCCAGTGGAGACA
  ACAGCTGGCAGCCAGGAGGCAGGAGCAGCGGGCGACAGTGCGGGCCCTGTGGTTCTGGGCCTTCTCGCTG
  CAGGCAAAGGTGTGGGCCACGTGGCTGGCCTTTGTACTGGAAAGGAGGAGAAAGAAGGCGCGGCTGCAGT
  GGGCGCTCCAGGCCTACCAGGGGCAGCTCCTCCAGGAGGGTGCCACGCGGCTCCTGCGCTTTGCAGCCAG
  CATGAAGGCCTCCCGGCAGCAGCTGCAGGCCCAGCAGCAGGTCCAGGCGGCTCACAGTCTCCATCGTGCC
  GTCCGCCGCTGTGCCACGCTCTGGAAACAGAAAGTGCTGGGCCGGGGCGGGAAGCCTCAGCCCCTGGCAG
  CCATCGCACCCAGCAGGAAAGTGACGTTTGAGGGTCCCCTTCTCAACCGCATTGCTGCTGGGGCTGGGGA
  TGGCACCCTTGAGACCAAGAGGCCACAGGCTTCTCGGCCTCTGGGAGCTCTGGGCCGCCTGGCTGCTGAG
  GAGCCCCACGCCCTGGAGCTCAACACTGCCCACTCAGCGAGGAAGCAGCCGCGACGCCCACACTTCCTGT
  TGGAGCCTGCGCAGAGCCAGAGGCCTCAGAAGCCACAGGAACATGGCCTAGGCATGGCTCAGCCAGCAGC
  CCCCTCCCTGACGCGGCCCTTCCTGGCAGAGGCCCCGACAGCACTGGTCCCACACAGCCCCCTGCCTGGG
  GCCCTGTCAAGCGCCCCTGGCCCGAAGCAGCCCCCGACGGCAAGCACAGGCCCGGAGCTGCTGCTGCTGC
  CTCTTTCCTCCTTCATGCCCTGCGGGGCGGCTGCACCAGCCAGGGTGTCAGCACAGCGGGCTACTCCTAG
  GGATAAGCCCCCGGTCCCCTCATCCCTGGCCAGTGTCCCTGACCCCCATCTACTCCTTCCTGGGGACTTC
  TCAGCCACCAGGGCTGGGCCTGGACTTTCAACTGCAGGCAGCCTGGACCTTGAGGCTGAACTTGAGGAGA
  TCCAGCAGCAACTACTGCACTACCAGACCACCAAGCAGAACCTCTGGTCCTGTCGGCGGCAAGCGAGCAG
  CCTGCGCAGGTGGCTGGAGCTGAACAGAGAGGAGCCGGGGCCTGAGGACCAGGAAGTAGAGCAGCAGGTG
  CAGAAAGAGCTGGAACAGGTGGAAATGCAGATCCAGCTGCTGGCAGAGGAGCTCCAGGCTCAGCGCCAGC
  CCATTGGCGCCTGCGTTGCCCGCATCCAGGCCCTGCGGCAGGCCCTGTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235199 representing NM_001258326
  Red=Cloning site Green=Tags(s)
  
  MKNLLTEKCISSHNFHQKVIKQRMEKKVDSRFYYEQRLLRKVFEEWKEEWWVFQHEWKLCVRADCHYRYY
  LYNLMFQTWKTYVRQQQEMRNKYIRAEVHDAKQKMRQAWKSWLIYVVVRRTKLQMQTTALEFRQRIILRV
  WWSTWRQRLGQVRVSRALHASALKHRALSLQVQAWSQWREQLLYVQKEKQKVVSAVKHHQHWQKRRFLKA
  WLEYLQVRRVKRQQNEMAERFHHVTVLQIYFCDWQQAWERRESLYAHHAQVEKLARKMALRRAFTHWKHY
  MLLCAEEAAQFEMAEEHHRHSQLYFCFRALKDNVTHAHLQQIRRNLAHQQHGVTLLHRFWNLWRSQIEQK
  KERELLPLLHAAWDHYRIALLCKCIELWLQYTQKRRYKQLLQARADGHFQQRALPAAFHTWNRLWRWRHQ
  ENVLSARATRFHRETLEKQVFSLWRQKMFQHRENRLAERMAILHAERQLLYRSWFMWHQQAAARHQEQEW
  QTVACAHHRHGRLKKAFCLWRESAQGLRTERTGRVRAAEFHMAQLLRWAWSQWRECLALRGAERQKLMRA
  DLHHQHSVLHRALQAWVTYQGRVRSILREVAARESQHNRQLLRGALRRWKENTMARVDEAKKTFQASTHY
  RRTICSKVLVQWREAVSVQMYYRQQEDCAIWEAQKVLDRGCLRTWFQRWWDCSRRSAQQRLQLERAVQHH
  HRQLLLEGLARWKTHHLQCVRKRLLHRQSTQLLAQRLSRTCFRQWRQQLAARRQEQRATVRALWFWAFSL
  QAKVWATWLAFVLERRRKKARLQWALQAYQGQLLQEGATRLLRFAASMKASRQQLQAQQQVQAAHSLHRA
  VRRCATLWKQKVLGRGGKPQPLAAIAPSRKVTFEGPLLNRIAAGAGDGTLETKRPQASRPLGALGRLAAE
  EPHALELNTAHSARKQPRRPHFLLEPAQSQRPQKPQEHGLGMAQPAAPSLTRPFLAEAPTALVPHSPLPG
  ALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLASVPDPHLLLPGDF
  SATRAGPGLSTAGSLDLEAELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQV
  QKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258326
• ORF Size: 3480 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258326.2
• RefSeq Size: 4141 bp
• RefSeq ORF: 3483 bp
• Locus ID: 9814
• UniProt ID: A8K8P3
• Cytogenetics: 22q12.2
• Gene Summary: Plays a role in the dynamic structure of centrosome-associated contractile fibers via its interaction with CETN2.[UniProtKB/Swiss-Prot Function]