RAGEF2 (RAPGEF6) (NM_001164386) Human Untagged Clone

CAT#: SC327717

RAPGEF6 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAPGEF6
• Synonyms: KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2; RAGEF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001164386, the custom clone sequence may differ by one or more nucleotides
  ATGAACTCACCCGTGGACCCTGGCGCTAGGCAGGCGTTGAGGAAGAAGCCACCCGAGCGG
  ACTCCCGAGGACTTAAATACTATTTATTCTTATCTTCATGGAATGGAAATATTATCAAAT
  CTCAGGGAACATCAGCTTAGATTAATGTCTGCAAGAGCACGCTATGAGAGATACAGTGGC
  AATCAGGTTCTCTTTTGTTCAGAAACGATTGCCAGATGTTGGTATATCCTACTTTCTGGA
  TCTGTGCTTGTGAAAGGCTCCATGGTCTTGCCTCCTTGCAGTTTTGGTAAGCAGTTTGGA
  GGAAAAAGAGGATGTGATTGTCTTGTATTAGAGCCTTCAGAAATGATTGTGGTAGAGAAT
  GCCAAAGATAATGAAGATAGTATTCTACAAAGAGAAATTCCTGCCAGACAATCCCGAAGA
  AGATTTCGGAAAATTAACTATAAAGGAGAGCGCCAAACCATTACTGATGATGTGGAGGTT
  AACAGCTATCTTTCTCTTCCAGCTGATCTTACCAAGATGCATCTCACAGAAAACCCTCAT
  CCACAGGTGACTCATGTGTCTTCTAGTCAGTCTGGTTGTAGCATTGCCAGTGACTCTGGA
  AGCAGCAGTTTATCTGATATCTATCAGGCTACGGAGAGTGAGGTAGGAGATGTAGATTTG
  ACACGTCTTCCAGAAGGACCTGTTGATTCTGAGGATGACGAAGAGGAAGATGAAGAGATT
  GATCGAACAGATCCATTGCAGGGGCGAGATCTTGTTCGAGAATGTCTTGAAAAAGAACCT
  GCAGACAAAACTGATGATGACATTGAACAATTGCTGGAGTTTATGCACCAGCTCCCTGCA
  TTTGCAAACATGACCATGTCTGTAAGGAGAGAACTCTGCTCAGTGATGATTTTTGAAGTG
  GTAGAGCAGGCTGGAGCTATTATTCTTGAAGATGGGCAAGAGCTTGACTCATGGTATGTT
  ATTTTAAACGGCACTGTGGAAATCAGTCATCCAGATGGAAAAGTTGAAAATTTGTTTATG
  GGAAATAGTTTTGGAATTACTCCCACTCTGGATAAGCAGTACATGCATGGAATTGTCAGG
  ACTAAAGTAGATGATTGTCAGTTTGTCTGCATAGCCCAGCAAGATTATTGGAGAATTTTA
  AACCATGTGGAAAAAAATACCCATAAAGTTGAGGAAGAGGGAGAAATTGTTATGGTACAT
  GAGCATCGGGAACTAGACCGGAGTGGAACCAGGAAAGGACACATTGTGATCAAGGCAACA
  CCTGAGCGTCTCATAATGCATTTAATAGAAGAACATTCCATCGTGGATCCAACTTATATA
  GAAGATTTTCTATTAACTTACAGGACATTTCTTGAAAGTCCTTTGGATGTTGGGATCAAA
  CTATTGGAATGGTTTAAGATCGACAGCTTAAGAGATAAGGTGACACGGATTGTATTATTA
  TGGGTAAATAATCATTTTAATGATTTTGAAGGTGACCCTGCTATGACTCGATTTCTAGAG
  GAATTTGAAAAAAATCTGGAAGATACAAAGATGAATGGTCATCTCCGGTTATTGAATATT
  GCCTGTGCTGCAAAGGCTAAGTGGAGACAGGTTGTGCTGCAAAAGGCTTCCCGCGAGTCC
  CCTCTACAATTCAGCCTTAATGGAGGGAGTGAGAAGGGATTTGGTATTTTTGTTGAAGGA
  GTAGAACCTGGTAGCAAAGCTGCTGATTCAGGACTGAAACGTGGTGATCAGATTATGGAA
  GTAAATGGACAAAACTTTGAGAATATTACATTTATGAAAGCCGTTGAAATTTTGAGGAAT
  AATACTCATCTTGCACTTACTGTGAAGACCAACATTTTTGTGTTCAAAGAGTTACTTTTT
  AGGACTGAACAAGAGAAATCTGGTGTTCCTCATATTCCCAAAATTGCTGAAAAAAAAAGT
  AATCGCCATTCTATCCAGCATGTGCCAGGAGATATTGAACAGACATCACAGGAGAAAGGA
  AGTAAGAAAGTTAAAGCAAATACTGTTTCAGGTGGAAGAAACAAAATCAGGAAGATTTTG
  GATAAAACACGATTTAGTATCTTGCCTCCAAAGCTATTTAGTGATGGAGGCCTAAGCCAA
  TCACAAGATGACAGCATTGTGGGAACAAGGCACTGTAGGCATAGTCTGGCTATAATGCCC
  ATCCCTGGAACACTCTCATCCAGCAGCCCTGATCTCCTGCAGCCTACCACCAGTATGTTG
  GATTTTTCCAATCCTTCAGATATCCCTGATCAAGTTATAAGAGTTTTCAAAGTGGATCAG
  CAAAGTTGCTACATTATCATCAGTAAAGACACCACAGCTAAAGAAGTAGTTTTTCATGCT
  GTTCATGAATTTGGTTTGACCGGTGCATCCGACACATATTCTCTCTGTGAAGTTTCTGTT
  ACTCCTGAGGGTGTCATAAAACAGAGAAGACTTCCAGATCAGTTCTCCAAATTAGCTGAT
  AGAATTCAACTCAATGGAAGGTATTACTTAAAAAATAACATGGAAACAGAAACCTTATGT
  TCAGATGAAGATGCTCAAGAACTAGTTAAGGAAAGCCAGCTATCCATGCTGCAGCTCAGT
  ACCATTGAGGTGGCCACCCAGCTGTCAATGAGGGACTTTGATTTGTTTCGTAATATTGAA
  CCGACTGAGTACATCGATGACCTTTTTAAGTTAAATTCCAAAACAGGAAATACTCATTTG
  AAGAGGTTTGAGGACATTGTAAACCAAGAGACATTCTGGGTTGCCTCAGAAATTTTAACT
  GAAGCAAATCAGCTCAAACGAATGAAGATTATTAAGCATTTTATTAAAATTGCACTTCAT
  TGTCGAGAATGTAAGAACTTCAATTCCATGTTTGCAATAATAAGTGGCTTGAACCTGGCA
  TCTGTAGCAAGACTCAGAGGAACTTGGGAAAAGTTACCAAGCAAATACGAGAAACATCTT
  CAAGATCTACAAGACATTTTTGATCCATCTAGAAACATGGCAAAGTATAGAAATATTCTT
  AGTAGTCAAAGTATGCAGCCTCCAATTATTCCACTCTTCCCTGTTGTCAAGAAAGATATG
  ACATTTCTACATGAAGGAAATGACTCCAAAGTAGATGGTTTAGTAAACTTTGAGAAGTTA
  AGAATGATTTCCAAGGAAATCCGCCAAGTTGTTCGAATGACTTCTGCTAACATGGACCCA
  GCTATGATGTTTCGACAGAGGAAGAAGAGGTGGCGGAGTCTGGGGTCACTGAGTCAAGGA
  AGCACAAATTCAAACATGCTGGATGTTCAGGGAGGTGCTCACAAAAAAAGGGCACGCCGC
  AGCTCTCTGCTTAATGCCAAGAAGCTATATGAGGATGCCCAAATGGCAAGGAAGGTGAAG
  CAGTATCTTTCCAGTCTCGATGTAGAGACAGATGAGGAGAAGTTCCAGATGATGTCATTA
  CAGTGGGAGCCTGCATATGGTACCTTGACCAAGAATTTAAGTGAGAAAAGATCAGCCAAA
  TCATCTGAAATGTCTCCAGTGCCTATGAGGTCAGCTGGCCAAACAACTAAAGCCCACTTG
  CATCAACCCCACAGAGTAAGCCAGGTGCTTCAGGTGCCAGCTGTTAATTTGCACCCCATC
  AGGAAGAAGGGACAAACAAAAGACCCTGCACTGAATACAAGTTTACCTCAGAAAGTTTTA
  GGAACAACTGAAGAAATAAGTGGTAAGAAGCATACAGAAGACACTATTTCTGTGGCGTCA
  TCTTTACATTCTAGTCCTCCTGCATCTCCTCAAGGCTCCCCTCACAAAGGTTACACACTT
  ATTCCATCAGCTAAATCTGACAACTTGTCTGACTCCAGCCATAGTGAGATTTCTTCACGG
  TCCAGCATCGTGAGCAATTGTTCTGTTGACTCCATGTCTGCAGCTCTACAGGATGAACGG
  TGTTCCTCTCAGGCCCTGGCAGTCCCTGAATCCACTGGGGCATTGGAAAAGACAGAGCAC
  GCTTCAGGGATAGGAGATCATAGTCAACATGGCCCTGGGTGGACACTCTTGAAGCCATCT
  CTAATCAAGTGTTTAGCTGTCTCATCGTCTGTGAGCAATGAAGAGATTTCTCAAGAGCAT
  ATCATTATAGAAGCAGCTGACAGTGGTCGTGGAAGTTGGACTTCGTGTTCAAGCAGCTCC
  CATGACAACTTCCAAAGCCTTCCAAACCCAAAAAGCTGGGATTTTTTGAACTCTTACAGA
  CATACCCATTTGGATGACCCCATTGCTGAAGTTGAACCCACTGACTCTGAGCCCTATTCC
  TGTTCTAAAAGCTGCTCTAGAACTTGTGGGCAGTGTAAAGGAAGCCTAGAGAGAAAGAGT
  TGGACCTCCTCCAGTTCTCTGTCTGACACGTATGAACCAAACTATGGGACAGTTAAACAG
  AGAGTATTGGAGAGCACCCCAGCTGAGTCATCTGAAGGCTTGGACCCCAAGGATGCCACT
  GACCCAGTTTATAAAACTGTCACTTCAAGTACAGAAAAGGGCTTGATTGTGTACTGTGTC
  ACCTCACCCAAGAAGGACGATAGGTATAGGGAGCCACCTCCCACTCCTCCAGGATATTTG
  GGGATTTCTTTAGCGGACCTAAAGGAAGGACCCCACACACACCTAAAACCTCCAGATTAT
  AGTGTGGCAGTGCAGAGGTCAAAGATGATGCATAACAGCCTCTCTAGACTGCCACCAGCT
  TCTCTCAGTAGCAACCTCGTGGCCTGTGTTCCATCGAAGATTGTAACTCAGCCTCAGAGG
  CATAATTTGCAGCCATTCCATCCTAAACTAGGAGATGTGACTGATGCAGATAGCGAAGCA
  GATGAAAATGAACAAGTTTCAGCAGTC
  
• Restriction Sites: Please inquire
• ACCN: NM_001164386
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164386.1, NP_001157858.1
• RefSeq Size: 8395 bp
• RefSeq ORF: 4830 bp
• Locus ID: 51735
• UniProt ID: Q8TEU7
• Cytogenetics: 5q31.1
• Gene Summary: Guanine nucleotide exchange factor (GEF) for Rap1A, Rap2A and M-Ras GTPases. Does not interact with cAMP.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).