RAGEF2 (RAPGEF6) (NM_001164386) Human Tagged ORF Clone
CAT#: RG229082
- TrueORF®
RAPGEF6 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant 1
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001164386" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | RAGEF2 |
Synonyms | KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2; RAGEF2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG229082 representing NM_001164386
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCACCCGTGGACCCTGGCGCTAGGCAGGCGTTGAGGAAGAAGCCACCCGAGCGGACTCCCGAGG ACTTAAATACTATTTATTCTTATCTTCATGGAATGGAAATATTATCAAATCTCAGGGAACATCAGCTTAG ATTAATGTCTGCAAGAGCACGCTATGAGAGATACAGTGGCAATCAGGTTCTCTTTTGTTCAGAAACGATT GCCAGATGTTGGTATATCCTACTTTCTGGATCTGTGCTTGTGAAAGGCTCCATGGTCTTGCCTCCTTGCA GTTTTGGTAAGCAGTTTGGAGGAAAAAGAGGATGTGATTGTCTTGTATTAGAGCCTTCAGAAATGATTGT GGTAGAGAATGCCAAAGATAATGAAGATAGTATTCTACAAAGAGAAATTCCTGCCAGACAATCCCGAAGA AGATTTCGGAAAATTAACTATAAAGGAGAGCGCCAAACCATTACTGATGATGTGGAGGTTAACAGCTATC TTTCTCTTCCAGCTGATCTTACCAAGATGCATCTCACAGAAAACCCTCATCCACAGGTGACTCATGTGTC TTCTAGTCAGTCTGGTTGTAGCATTGCCAGTGACTCTGGAAGCAGCAGTTTATCTGATATCTATCAGGCT ACGGAGAGTGAGGTAGGAGATGTAGATTTGACACGTCTTCCAGAAGGACCTGTTGATTCTGAGGATGACG AAGAGGAAGATGAAGAGATTGATCGAACAGATCCATTGCAGGGGCGAGATCTTGTTCGAGAATGTCTTGA AAAAGAACCTGCAGACAAAACTGATGATGACATTGAACAATTGCTGGAGTTTATGCACCAGCTCCCTGCA TTTGCAAACATGACCATGTCTGTAAGGAGAGAACTCTGCTCAGTGATGATTTTTGAAGTGGTAGAGCAGG CTGGAGCTATTATTCTTGAAGATGGGCAAGAGCTTGACTCATGGTATGTTATTTTAAACGGCACTGTGGA AATCAGTCATCCAGATGGAAAAGTTGAAAATTTGTTTATGGGAAATAGTTTTGGAATTACTCCCACTCTG GATAAGCAGTACATGCATGGAATTGTCAGGACTAAAGTAGATGATTGTCAGTTTGTCTGCATAGCCCAGC AAGATTATTGGAGAATTTTAAACCATGTGGAAAAAAATACCCATAAAGTTGAGGAAGAGGGAGAAATTGT TATGGTACATGAGCATCGGGAACTAGACCGGAGTGGAACCAGGAAAGGACACATTGTGATCAAGGCAACA CCTGAGCGTCTCATAATGCATTTAATAGAAGAACATTCCATCGTGGATCCAACTTATATAGAAGATTTTC TATTAACTTACAGGACATTTCTTGAAAGTCCTTTGGATGTTGGGATCAAACTATTGGAATGGTTTAAGAT CGACAGCTTAAGAGATAAGGTGACACGGATTGTATTATTATGGGTAAATAATCATTTTAATGATTTTGAA GGTGACCCTGCTATGACTCGATTTCTAGAGGAATTTGAAAAAAATCTGGAAGATACAAAGATGAATGGTC ATCTCCGGTTATTGAATATTGCCTGTGCTGCAAAGGCTAAGTGGAGACAGGTTGTGCTGCAAAAGGCTTC CCGCGAGTCCCCTCTACAATTCAGCCTTAATGGAGGGAGTGAGAAGGGATTTGGTATTTTTGTTGAAGGA GTAGAACCTGGTAGCAAAGCTGCTGATTCAGGACTGAAACGTGGTGATCAGATTATGGAAGTAAATGGAC AAAACTTTGAGAATATTACATTTATGAAAGCCGTTGAAATTTTGAGGAATAATACTCATCTTGCACTTAC TGTGAAGACCAACATTTTTGTGTTCAAAGAGTTACTTTTTAGGACTGAACAAGAGAAATCTGGTGTTCCT CATATTCCCAAAATTGCTGAAAAAAAAAGTAATCGCCATTCTATCCAGCATGTGCCAGGAGATATTGAAC AGACATCACAGGAGAAAGGAAGTAAGAAAGTTAAAGCAAATACTGTTTCAGGTGGAAGAAACAAAATCAG GAAGATTTTGGATAAAACACGATTTAGTATCTTGCCTCCAAAGCTATTTAGTGATGGAGGCCTAAGCCAA TCACAAGATGACAGCATTGTGGGAACAAGGCACTGTAGGCATAGTCTGGCTATAATGCCCATCCCTGGAA CACTCTCATCCAGCAGCCCTGATCTCCTGCAGCCTACCACCAGTATGTTGGATTTTTCCAATCCTTCAGA TATCCCTGATCAAGTTATAAGAGTTTTCAAAGTGGATCAGCAAAGTTGCTACATTATCATCAGTAAAGAC ACCACAGCTAAAGAAGTAGTTTTTCATGCTGTTCATGAATTTGGTTTGACCGGTGCATCCGACACATATT CTCTCTGTGAAGTTTCTGTTACTCCTGAGGGTGTCATAAAACAGAGAAGACTTCCAGATCAGTTCTCCAA ATTAGCTGATAGAATTCAACTCAATGGAAGGTATTACTTAAAAAATAACATGGAAACAGAAACCTTATGT TCAGATGAAGATGCTCAAGAACTAGTTAAGGAAAGCCAGCTATCCATGCTGCAGCTCAGTACCATTGAGG TGGCCACCCAGCTGTCAATGAGGGACTTTGATTTGTTTCGTAATATTGAACCGACTGAGTACATCGATGA CCTTTTTAAGTTAAATTCCAAAACAGGAAATACTCATTTGAAGAGGTTTGAGGACATTGTAAACCAAGAG ACATTCTGGGTTGCCTCAGAAATTTTAACTGAAGCAAATCAGCTCAAACGAATGAAGATTATTAAGCATT TTATTAAAATTGCACTTCATTGTCGAGAATGTAAGAACTTCAATTCCATGTTTGCAATAATAAGTGGCTT GAACCTGGCATCTGTAGCAAGACTCAGAGGAACTTGGGAAAAGTTACCAAGCAAATACGAGAAACATCTT CAAGATCTACAAGACATTTTTGATCCATCTAGAAACATGGCAAAGTATAGAAATATTCTTAGTAGTCAAA GTATGCAGCCTCCAATTATTCCACTCTTCCCTGTTGTCAAGAAAGATATGACATTTCTACATGAAGGAAA TGACTCCAAAGTAGATGGTTTAGTAAACTTTGAGAAGTTAAGAATGATTTCCAAGGAAATCCGCCAAGTT GTTCGAATGACTTCTGCTAACATGGACCCAGCTATGATGTTTCGACAGAGGAAGAAGAGGTGGCGGAGTC TGGGGTCACTGAGTCAAGGAAGCACAAATTCAAACATGCTGGATGTTCAGGGAGGTGCTCACAAAAAAAG GGCACGCCGCAGCTCTCTGCTTAATGCCAAGAAGCTATATGAGGATGCCCAAATGGCAAGGAAGGTGAAG CAGTATCTTTCCAGTCTCGATGTAGAGACAGATGAGGAGAAGTTCCAGATGATGTCATTACAGTGGGAGC CTGCATATGGTACCTTGACCAAGAATTTAAGTGAGAAAAGATCAGCCAAATCATCTGAAATGTCTCCAGT GCCTATGAGGTCAGCTGGCCAAACAACTAAAGCCCACTTGCATCAACCCCACAGAGTAAGCCAGGTGCTT CAGGTGCCAGCTGTTAATTTGCACCCCATCAGGAAGAAGGGACAAACAAAAGACCCTGCACTGAATACAA GTTTACCTCAGAAAGTTTTAGGAACAACTGAAGAAATAAGTGGTAAGAAGCATACAGAAGACACTATTTC TGTGGCGTCATCTTTACATTCTAGTCCTCCTGCATCTCCTCAAGGCTCCCCTCACAAAGGTTACACACTT ATTCCATCAGCTAAATCTGACAACTTGTCTGACTCCAGCCATAGTGAGATTTCTTCACGGTCCAGCATCG TGAGCAATTGTTCTGTTGACTCCATGTCTGCAGCTCTACAGGATGAACGGTGTTCCTCTCAGGCCCTGGC AGTCCCTGAATCCACTGGGGCATTGGAAAAGACAGAGCACGCTTCAGGGATAGGAGATCATAGTCAACAT GGCCCTGGGTGGACACTCTTGAAGCCATCTCTAATCAAGTGTTTAGCTGTCTCATCGTCTGTGAGCAATG AAGAGATTTCTCAAGAGCATATCATTATAGAAGCAGCTGACAGTGGTCGTGGAAGTTGGACTTCGTGTTC AAGCAGCTCCCATGACAACTTCCAAAGCCTTCCAAACCCAAAAAGCTGGGATTTTTTGAACTCTTACAGA CATACCCATTTGGATGACCCCATTGCTGAAGTTGAACCCACTGACTCTGAGCCCTATTCCTGTTCTAAAA GCTGCTCTAGAACTTGTGGGCAGTGTAAAGGAAGCCTAGAGAGAAAGAGTTGGACCTCCTCCAGTTCTCT GTCTGACACGTATGAACCAAACTATGGGACAGTTAAACGGAGAGTATTGGAGAGCACCCCAGCTGAGTCA TCTGAAGGCTTGGACCCCAAGGATGCCACTGACCCAGTTTATAAAACTGTCACTTCAAGTACAGAAAAGG GCTTGATTGTGTACTGTGTCACCTCACCCAAGAAGGACGATAGGTATAGGGAGCCACCTCCCACTCCTCC AGGATATTTGGGGATTTCTTTAGCGGACCTAAAGGAAGGACCCCACACACACCTAAAACCTCCAGATTAT AGTGTGGCAGTGCAGAGGTCAAAGATGATGCATAACAGCCTCTCTAGACTGCCACCAGCTTCTCTCAGTA GCAACCTCGTGGCCTGTGTTCCATCGAAGATTGTAACTCAGCCTCAGAGGCATAATTTGCAGCCATTCCA TCCTAAACTAGGAGATGTGACTGATGCAGATAGCGAAGCAGATGAAAATGAACAAGTTTCAGCAGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229082 representing NM_001164386
Red=Cloning site Green=Tags(s) MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETI ARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRR RFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQA TESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPA FANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTL DKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKAT PERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFE GDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEG VEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVP HIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQ SQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKD TTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLC SDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQE TFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHL QDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQV VRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVK QYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVL QVPAVNLHPIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKGYTL IPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIGDHSQH GPGWTLLKPSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQSLPNPKSWDFLNSYR HTHLDDPIAEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSSSSLSDTYEPNYGTVKRRVLESTPAES SEGLDPKDATDPVYKTVTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYLGISLADLKEGPHTHLKPPDY SVAVQRSKMMHNSLSRLPPASLSSNLVACVPSKIVTQPQRHNLQPFHPKLGDVTDADSEADENEQVSAV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001164386 |
ORF Size | 4827 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001164386.1, NP_001157858.1 |
RefSeq Size | 8395 bp |
RefSeq ORF | 4830 bp |
Locus ID | 51735 |
UniProt ID | Q8TEU7 |
Cytogenetics | 5q31.1 |
Gene Summary | Guanine nucleotide exchange factor (GEF) for Rap1A, Rap2A and M-Ras GTPases. Does not interact with cAMP.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC229082 | RAPGEF6 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant 1 |
USD 1,514.00 |
|
RC229082L3 | Lenti-ORF clone of RAPGEF6 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant 1 |
USD 1,814.00 |
|
RC229082L4 | Lenti-ORF clone of RAPGEF6 (mGFP-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant 1 |
USD 1,814.00 |
|
SC327717 | RAPGEF6 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6) transcript variant 1 |
USD 1,515.00 |
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