GPCR TGR7 (MRGPRD) (NM_198923) Human Untagged Clone

CAT#: SC309807

MRGPRD (untagged)-Human MAS-related GPR, member D (MRGPRD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPCR TGR7
• Synonyms: MRGD; TGR7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_198923 edited
  GGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTAAGGCATGAACCAGACTTTG
  AATAGCAGTGGGACCGTGGAGTCAGCCCTAAACTATTCCAGAGGGAGCACAGTGCACACG
  GCCTACCTGGTGCTGAGCTCCCTGGCCATGTTCACCTGCCTGTGCGGGATGGCAGGCAAC
  AGCATGGTGATCTGGCTGCTGGGCTTTCGAATGCACAGGAACCCCTTCTGCATCTATATC
  CTCAACCTGGCGGCAGCCGACCTCCTCTTCCTCTTCAGCATGGCTTCCACGCTCAGCCTG
  GAAACCCAGCCCCTGGTCAATACCACTGACAAGGTCCACGAGCTGATGAAGAGACTGATG
  TACTTTGCCTACACAGTGGGCCTGAGCCTGCTGACGGCCATCAGCACCCAGCGCTGTCTC
  TCTGTCCTCTTCCCTATCTGGTTCAAGTGTCACCGGCCCAGGCACCTGTCAGCCTGGGTG
  TGTGGCCTGCTGTGGACGCTCTGTCTCCTGATGAACGGGTTGACCTCTTCCTTCTGCAGC
  AAGTTCTTGAAATTCAATGAAGATCGGTGCTTCAGGGTGGACATGGTCCAGGCCGCCCTC
  ATCATGGGGGTCTTAACCCCAGTGATGACTCTGTCCAGCCTGACCCTCTTTGTCTGGGTG
  CGGAGGAGCTCCCAGCAGTGGCGGCGGCAGCCCACACGGCTGTTCGTGGTGGTCCTGGCC
  TCTGTCCTGGTGTTCCTCATCTGTTCCCTGCCTCTGAGCATCTACTGGTTTGTGCTCTAC
  TGGTTGAGCCTGCCGCCCGAGATGCAGGTCCTGTGCTTCAGCTTGTCACGCCTCTCCTCG
  TCCGTAAGCAGCAGCGCCAACCCCGTCATCTACTTCCTGGTGGGCAGCCGGAGGAGCCAC
  AGGCTGCCCACCAGGTCCCTGGGGACTGTGCTCCAACAGGCGCTTCGCGAGGAGCCCGAG
  CTGGAAGGTGGGGAGACGCCCACCGTGGGCACCAATGAGATGGGGGCTTGAGAAAGCCGA
  ATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCGATATCAAGCTTG
  TCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCC
  CTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAG
  CCTTGTCCTAATAAAATTAAATTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATAT
  TATGGGGTGG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198923 unedited
  TGGGGGCAACTGGATTTGTAAACGACATACTATAGGCGGCCGCCAGTGTGATGGATATCT
  GCAGAATTCGGCTTAAGGCATGAACCAGACTTTGAATAGCAGTGGGACCGTGGAGTCAGC
  CCTAAACTATTCCAGAGGGAGCACAGTGCACACGGCCTACCTGGTGCTGAGCTCCCTGGC
  CATGTTCACCTGCCTGTGCGGGATGGCAGGCAACAGCATGGTGATCTGGCTGCTGGGCTT
  TCGAATGCACAGGAACCCCTTCTGCATCTATATCCTCAACCTGGCGGCAGCCGACCTCCT
  CTTCCTCTTCAGCATGGCTTCCACGCTCAGCCTGGAAACCCAGCCCCTGGTCAATACCAC
  TGACAAGGTCCACGAGCTGATGAAGAGACTGATGTACTTTGCCTACACAGTGGGCCTGAG
  CCTGCTGACGGCCATCAGCACCCAGCGCTGTCTCTCTGTCCTCTTCCCTATCTGGTTCAA
  GTGTCACCGGCCCAGGCACCTGTCAGCCTGGGTGTGTGGCCTGCTGTGGACGCTCTGTCT
  CCTGATGAACGGGTTGACCTCTTCCTTCTGCAGCAAGTTCTTGAAATTCAATGAAGATCG
  GTGCTTCAGGGTGGACATGGTCCAGGCCGCCCTCATCATGGGGGTCTTAACCCCAGTGAT
  GACTCTGTCCAGCCTGACCCTCTTTGTCTGGGTGCGGAGGAGCTCCCAGCAGTGGCGGCG
  GCAGCCCACACGGCTGTTCGTGGTGGTCCTGGCCTCTGTCCTGGTGTTCCTCATCTGTTC
  CCTGCCTCTGAGCATCTACTGGTTTGTGCTCTACTGGTTGAGCCTGCCGCCCGAGATGCA
  NGTCCTGTGCTTCAGCTTGTCACGCCTCTCCTCGTCCGTAAGCAGCAGCGCCAACC
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_198923 unedited
  GAACTAGGGGTCTAACCCATGAGACTTGTCCAGCCGNCCCTCTTTGTCTGGGTGCGGAGG
  AGCTCCCAGCAGTGGCGGCGGCAGCCCACACGGCTGTTCGTGGTGGTCCTGGCCTCTGTC
  CTGGTGTTCCTCATCTGTTCCCTGCCTCTGAGCATCTACTGGTTTGTGCTCTACTGGTTG
  AGCCTGCCGCCCGAGATGCAGGTCCTGTGCTTCAGCTTGTCACGCCTCTCCTCGTCCGTA
  AGCAGCAGCGCCAACCCCGTCATCTACTTCCTGGTGGGCAGNCNGAGGAGCCACAGGCTG
  CCCACCAGGTCCCTGGGGACTGNGCTCCAACAGGCGCTTCGCGAGGAGCCCGAGCTGGAA
  GGTGGGGAGACGCCCACCGTGGGCACCAATGAGATGGGGGCTTGAGAAAGCCGAATTCCA
  GCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCGATATCAAGCTTGTCGACT
  CTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGA
  CCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCCTGT
  CCTAATAAAATTAAATTGCATCATTTTGTCTGACTAAGTGTCCTTCTATAATATTATTGN
  GTTGGAAGGGGGATGNTTNNAATNNAACNCAAATAANAAAACATAAAAAANNNNAACAAN
  AAAAAAAATNAAACNNNNCNNTNNNNNAAAATNAAACATAANNNNAAAANNNAAAANCAT
  AATAACAAANNAAAANAATAAAAAANAAACANCNAAANTAAAAANNAAAAAAAAAANACA
  NAAAAAAAAAAAATATAATAATATAAAAATTTANAAAANTNANGAAAAATTTGAAGAGAA
  GG
  
• Restriction Sites: Please inquire
• ACCN: NM_198923
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This construct is a cloned PCR fragment that was fully sequenced. There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF, however, this results in the substitution of no amino acids.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198923.2, NP_944605.2
• RefSeq Size: 966 bp
• RefSeq ORF: 966 bp
• Locus ID: 116512
• UniProt ID: Q8TDS7
• Cytogenetics: 11q13.3
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: May regulate nociceptor function and/or development, including the sensation or modulation of pain. Functions as a specific membrane receptor for beta-alanine. Beta-alanine at micromolar doses specifically evoked Ca(2+) influx in cells expressing the receptor. Beta-alanine decreases forskolin-stimulated cAMP production in cells expressing the receptor, suggesting that the receptor couples with G-protein G(q) and G(i).[UniProtKB/Swiss-Prot Function]