GPCR TGR7 (MRGPRD) (NM_198923) Human Tagged ORF Clone

CAT#: RC224023

MRGPRD (Myc-DDK-tagged)-Human MAS-related GPR, member D (MRGPRD)

Product Images

Circular map for RC224023

• 

  Western blot validation of overexpression lysate (Cat# LY403698) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224023 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPCR TGR7
• Synonyms: MRGD; TGR7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224023 representing NM_198923
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCAGACTTTGAATAGCAGTGGGACCGTGGAGTCAGCCCTAAACTATTCCAGAGGGAGCACAGTGC
  ACACGGCCTACCTGGTGCTGAGCTCCCTGGCCATGTTCACCTGCCTGTGCGGGATGGCAGGCAACAGCAT
  GGTGATCTGGCTGCTGGGCTTTCGAATGCACAGGAACCCCTTCTGCATCTATATCCTCAACCTGGCGGCA
  GCCGACCTCCTCTTCCTCTTCAGCATGGCTTCCACGCTCAGCCTGGAAACCCAGCCCCTGGTCAATACCA
  CTGACAAGGTCCACGAGCTGATGAAGAGACTGATGTACTTTGCCTACACAGTGGGCCTGAGCCTGCTGAC
  GGCCATCAGCACCCAGCGCTGTCTCTCTGTCCTCTTCCCTATCTGGTTCAAGTGTCACCGGCCCAGGCAC
  CTGTCAGCCTGGGTGTGTGGCCTGCTGTGGACGCTCTGTCTCCTGATGAACGGGTTGACCTCTTCCTTCT
  GCAGCAAGTTCTTGAAATTCAATGAAGATCGGTGCTTCAGGGTGGACATGGTCCAGGCCGCCCTCATCAT
  GGGGGTCTTAACCCCAGTGATGACTCTGTCCAGCCTGACCCTCTTTGTCTGGGTGCGGAGGAGCTCCCAG
  CAGTGGCGGCGGCAGCCCACACGGCTGTTCGTGGTGGTCCTGGCCTCTGTCCTGGTGTTCCTCATCTGTT
  CCCTGCCTCTGAGCATCTACTGGTTTGTGCTCTACTGGTTGAGCCTGCCGCCCGAGATGCAGGTCCTGTG
  CTTCAGCTTGTCACGCCTCTCCTCGTCCGTAAGCAGCAGCGCCAACCCCGTCATCTACTTCCTGGTGGGC
  AGCCGGAGGAGCCACAGGCTGCCCACCAGGTCCCTGGGGACTGTGCTCCAACAGGCGCTTCGCGAGGAGC
  CCGAGCTGGAAGGTGGGGAGACGCCCACCGTGGGCACCAATGAGATGGGGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224023 representing NM_198923
  Red=Cloning site Green=Tags(s)
  
  MNQTLNSSGTVESALNYSRGSTVHTAYLVLSSLAMFTCLCGMAGNSMVIWLLGFRMHRNPFCIYILNLAA
  ADLLFLFSMASTLSLETQPLVNTTDKVHELMKRLMYFAYTVGLSLLTAISTQRCLSVLFPIWFKCHRPRH
  LSAWVCGLLWTLCLLMNGLTSSFCSKFLKFNEDRCFRVDMVQAALIMGVLTPVMTLSSLTLFVWVRRSSQ
  QWRRQPTRLFVVVLASVLVFLICSLPLSIYWFVLYWLSLPPEMQVLCFSLSRLSSSVSSSANPVIYFLVG
  SRRSHRLPTRSLGTVLQQALREEPELEGGETPTVGTNEMGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198923
• ORF Size: 963 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198923.2, NP_944605.2
• RefSeq Size: 966 bp
• RefSeq ORF: 966 bp
• Locus ID: 116512
• UniProt ID: Q8TDS7
• Cytogenetics: 11q13.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 35.9 kDa
• Gene Summary: May regulate nociceptor function and/or development, including the sensation or modulation of pain. Functions as a specific membrane receptor for beta-alanine. Beta-alanine at micromolar doses specifically evoked Ca(2+) influx in cells expressing the receptor. Beta-alanine decreases forskolin-stimulated cAMP production in cells expressing the receptor, suggesting that the receptor couples with G-protein G(q) and G(i).[UniProtKB/Swiss-Prot Function]