Intersectin 2 (ITSN2) (NM_006277) Human Untagged Clone

CAT#: SC309767

ITSN2 (untagged)-Human intersectin 2 (ITSN2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Intersectin 2
• Synonyms: PRO2015; SH3D1B; SH3P18; SWA; SWAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC309767 representing NM_006277.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATGGCTCAGTTTCCCACAGCTATGAATGGAGGGCCAAACATGTGGGCTATTACCTCTGAAGAACGT
  ACTAAGCATGACAGGCAGTTTGATAACCTCAAACCTTCAGGAGGTTACATAACAGGTGATCAAGCACGT
  AATTTTTTCCTACAATCAGGTCTGCCGGCCCCTGTTTTAGCTGAAATATGGGCTTTATCAGACCTAAAC
  AAGGATGGGAAGATGGATCAGCAAGAGTTCTCCATAGCTATGAAACTCATCAAACTGAAGCTTCAAGGC
  CAACAGTTGCCTGTGGTTCTCCCTCCTATTATGAAGCAACCCCCTATGTTTTCTCCATTAATTTCTGCT
  CGTTTTGGAATGGGAAGCATGCCCAATCTGTCCATTCCTCAGCCATTGCCTCCAGCTGCACCTATAACA
  TCATTGTCTTCTGCGACTTCAGGGACCAACCTTCCTCCCTTAATGATGCCCACTCCCCTAGTGCCTTCT
  GTTAGCACATCATCATTACCAAATGGAACCGCCAGTCTCATTCAGCCTTTACCCATTCCTTATTCTTCT
  TCAACATTGCCTCATGGGTCATCTTATAGTCTGATGATGGGAGGATTTGGAGGTGCTAGTATACAGAAA
  GCGCAGTCTCTGATTGATTTAGGATCTAGTAGCTCAACTTCCTCGACTGCTTCACTCTCAGGGAACTCA
  CCCAAGACTGGGACCTCAGAGTGGGCAGTTCCTCAGCCTACAAGATTAAAATATCGGCAAAAATTTAAT
  ACTCTTGACAAAAGTATGAGTGGATATCTCTCAGGTTTTCAAGCTAGAAATGCCCTTCTTCAGTCAAAT
  CTTTCTCAAACTCAGCTGGCTACTATTTGGACTCTGGCTGACGTTGATGGTGATGGACAGCTAAAAGCA
  GAAGAGTTTATTCTTGCAATGCACCTTACTGACATGGCCAAAGCTGGACAGCCATTACCACTGACTTTA
  CCTCCTGAGCTTGTTCCTCCATCTTTCAGAGGAGGAAAGCAAATTGATTCCATTAATGGAACTCTGCCT
  TCATATCAGAAAATGCAAGAAGAGGAGCCTCAGAAGAAATTACCAGTTACTTTTGAGGACAAACGGAAA
  GCCAACTATGAGCGAGGGAACATGGAGCTGGAAAAGCGACGCCAAGCCTTGATGGAGCAGCAACAAAGG
  GAGGCAGAACGTAAAGCCCAGAAAGAAAAGGAAGAGTGGGAACGAAAACAGAGAGAATTACAAGAACAA
  GAATGGAAGAAACAACTTGAATTAGAAAAACGCTTAGAGAAGCAACGGGAATTGGAGAGACAACGAGAG
  GAAGAAAGGAGAAAAGACATAGAAAGACGAGAGGCAGCAAAACAGGAACTTGAACGACAACGTCGCTTA
  GAATGGGAGAGAATTCGGCGACAGGAGCTTCTCAATCAAAAGAATAGAGAACAAGAAGAAATTGTCAGG
  TTAAACTCTAAAAAGAAGAATCTTCATCTTGAGTTGGAAGCACTGAATGGCAAACATCAGCAGATCTCA
  GGCAGACTTCAGGATGTCCGACTCAAAAAGCAAACTCAAAAGACTGAGCTGGAAGTTCTGGATAAGCAG
  TGTGACTTGGAAATTATGGAAATCAAGCAACTTCAACAGGAACTTCAGGAATATCAGAATAAGCTTATC
  TATCTGGTACCTGAGAAGCAATTATTAAATGAAAGAATTAAAAACATGCAGTTCAGTAACACACCTGAT
  TCAGGGGTCAGTTTACTTCATAAAAAATCATTAGAAAAGGAAGAATTATGCCAAAGACTTAAAGAACAG
  TTAGATGCTCTTGAAAAAGAAACTGCATCTAAGCTGTCAGAAATGGATTCTTTTAACAATCAACTAAAG
  TGTGGGAATATGGATGACTCTGTTCTTCAGTGCCTTTTGTCTCTGCTAAGCTGTCTCAACAACCTCTTC
  CTCTTACTTAAGGAACTGAGAGAAACCTACAACACACAGCAGTTAGCCCTTGAACAGCTTTATAAGATC
  AAACGTGACAAGTTGAAGGAAATTGAAAGGAAAAGATTAGAACTAATGCAGAAAAAGAAACTAGAAGAT
  GAGGCTGCAAGGAAAGCAAAGCAAGGAAAAGAAAACTTATGGAAAGAAAATCTTAGAAAGGAGGAAGAA
  GAAAAACAAAAGCGACTCCAGGAAGAAAAAACACAAGAAAAAATTCAAGAAGAGGAACGGAAAGCTGAG
  GAGAAACAACGTAAGGATAAGGATACTTTGAAAGCTGAGGAGAAAAAACGTGAGACAGCTAGTGTTTTG
  GTGAATTATAGAGCATTATACCCCTTTGAAGCAAGGAACCATGATGAGATGAGTTTTAATTCTGGAGAT
  ATAATTCAGGTTGATGAAAAAACCGTAGGAGAACCTGGTTGGCTTTATGGTAGTTTTCAAGGAAATTTT
  GGCTGGTTTCCATGCAATTATGTAGAAAAAATGCCATCAAGTGAAAATGAAAAAGCTGTATCTCCAAAG
  AAGGCCTTACTTCCTCCTACAGTTTCTTTATCTGCTACCTCAACTTCCTCTGAACCACTTTCTTCAAAT
  CAACCAGCATCAGTGACTGATTATCAAAATGTATCTTTTTCAAACCTAACTGTAAATACATCATGGCAG
  AAAAAATCAGCCTTCACTCGAACTGTGTCCCCTGGATCTGTATCACCTATTCATGGACAGGGACAAGTG
  GTAGAAAACTTAAAAGCACAGGCCCTTTGTTCCTGGACTGCAAAGAAAGATAACCACTTGAACTTCTCA
  AAACATGACATTATTACTGTCTTGGAGCAGCAAGAAAATTGGTGGTTTGGGGAGGTGCATGGAGGAAGA
  GGATGGTTTCCCAAATCTTATGTCAAGATCATTCCTGGGAGTGAAGTAAAACGGGAAGAACCAGAAGCT
  TTGTATGCAGCTGTAAATAAGAAACCTACCTCGGCAGCCTATTCAGTTGGAGAAGAATATATTGCACTT
  TATCCATATTCAAGTGTGGAACCTGGAGATTTGACTTTCACAGAAGGTGAAGAAATATTGGTGACCCAG
  AAAGATGGAGAGTGGTGGACAGGAAGTATTGGAGATAGAAGTGGAATTTTTCCATCAAACTATGTCAAA
  CCAAAGGATCAAGAGAGTTTTGGGAGTGCTAGCAAGTCTGGAGCATCAAATAAAAAACCTGAGATTGCT
  CAGGTAACTTCAGCATATGTTGCTTCTGGTTCTGAACAACTTAGCCTTGCACCAGGACAGTTAATATTA
  ATTCTAAAGAAAAATACAAGTGGGTGGTGGCAAGGAGAGTTACAGGCCAGAGGAAAAAAGCGACAGAAA
  GGATGGTTTCCTGCCAGTCATGTTAAACTTTTGGGTCCAAGTAGTGAAAGAGCCACACCTGCCTTTCAT
  CCTGTATGTCAGGTGATTGCTATGTATGACTATGCAGCAAATAATGAAGATGAGCTCAGTTTCTCCAAG
  GGACAACTCATTAATGTTATGAACAAAGATGATCCTGATTGGTGGCAAGGAGAGATCAACGGGGTGACT
  GGTCTCTTTCCTTCAAACTACGTTAAGATGACGACAGACTCAGATCCAAGTCAACAGTGGTGTGCTGAT
  CTGCAAACCCTGGACACAATGCAGCCAATTGAGAGGAAAAGACAGGGCTATATTCATGAGCTGATTCAG
  ACCGAAGAGCGGTACATGGCTGACCTTCAGCTCGTCGTCGAGGTTTTTCAGAAACGCATGGCAGAGTCA
  GGCTTTCTCACTGAAGGGGAGATGGCCCTGATTTTTGTTAACTGGAAGGAGCTCATCATGTCCAACACA
  AAGCTGCTGAAGGCTTTGCGGGTGCGGAAGAAGACCGGGGGCGAGAAGATGCCGGTGCAGATGATTGGG
  GACATCCTGGCCGCTGAGCTGTCCCACATGCAGGCTTACATCAGGTTCTGCAGCTGCCAGCTTAATGGA
  GCAGCTCTGTTACAGCAGAAGACAGATGAAGACACAGATTTCAAAGAATTTTTAAAGAAGCTGGCATCT
  GACCCGCGGTGTAAAGGAATGCCCCTCTCCAGCTTCCTGCTGAAACCCATGCAGAGGATCACCCGCTAC
  CCACTGCTCATCAGAAGTATTCTGGAGAACACCCCGGAGAGCCATGCAGACCATTCCTCCCTAAAGCTG
  GCCCTCGAGCGGGCAGAGGAGCTGTGCTCTCAAGTGAATGAGGGAGTTCGGGAGAAGGAAAACTCGGAC
  CGACTGGAGTGGATCCAGGCGCACGTGCAGTGTGAAGGCCTCGCGGAGCAACTTATTTTCAACTCTCTC
  ACCAACTGCCTGGGGCCCCGGAAGCTCTTACACAGTGGGAAATTATACAAGACCAAGAGCAACAAGGAA
  CTGCACGGATTCCTCTTCAATGACTTCCTGCTTCTTACCTACATGGTCAAGCAGTTTGCTGTTTCCTCT
  GGCTCTGAGAAACTTTTCAGCTCGAAGTCCAATGCTCAATTCAAAATGTATAAAACGCCCATTTTCCTG
  AATGAAGTCTTGGTGAAACTGCCCACAGACCCTTCCAGCGATGAGCCTGTCTTCCACATTTCCCACATT
  GATCGGGTCTACACCCTCCGAACAGACAACATTAATGAGAGGACCGCCTGGGTGCAGAAGATCAAGGCG
  GCGTCTGAGCAGTACATCGACACCGAGAAGAAGAAGCGTGAGAAAGCTTACCAAGCCCGCTCCCAAAAG
  ACTTCAGGCATTGGGCGCCTGATGGTGCATGTCATTGAAGCTACAGAATTAAAAGCCTGCAAACCAAAT
  GGAAAGAGCAACCCATACTGTGAAATCAGCATGGGCTCCCAGAGCTACACCACCAGGACCATCCAGGAC
  ACACTCAATCCCAAGTGGAATTTTAACTGCCAGTTCTTTATTAAGGATCTCTACCAAGACGTGCTGTGT
  CTCACCCTGTTTGACAGAGACCAGTTTTCACCAGATGATTTCCTGGGTCGTACTGAAATTCCAGTGGCA
  AAAATTCGAACAGAACAGGAAAGCAAAGGCCCTATGACCCGCCGACTGCTGCTGCATGAGGTCCCCACC
  GGGGAGGTCTGGGTCCGTTTTGACCTGCAGCTTTTTGAGCAAAAAACTCTCCTGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_006277
• Insert Size: 5094 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006277.2
• RefSeq Size: 6112 bp
• RefSeq ORF: 5094 bp
• Locus ID: 50618
• UniProt ID: Q9NZM3
• Cytogenetics: 2p23.3
• Protein Families: Druggable Genome
• MW: 193.5 kDa
• Gene Summary: This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. [provided by RefSeq, Jan 2017]
  Transcript Variant: This variant (1), also known as ITSN2-L, encodes the longest isoform (1).