Intersectin 2 (ITSN2) (NM_006277) Human Tagged ORF Clone

CAT#: RC229449

ITSN2 (Myc-DDK-tagged)-Human intersectin 2 (ITSN2), transcript variant 1

Product Images

Circular map for RC229449

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Intersectin 2
• Synonyms: PRO2015; SH3D1B; SH3P18; SWA; SWAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229449 representing NM_006277
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCTCAGTTTCCCACAGCTATGAATGGAGGGCCAAACATGTGGGCTATTACCTCTGAAGAACGTA
  CTAAGCATGACAGGCAGTTTGATAACCTCAAACCTTCAGGAGGTTACATAACAGGTGATCAAGCACGTAA
  TTTTTTCCTACAATCAGGTCTGCCGGCCCCTGTTTTAGCTGAAATATGGGCTTTATCAGACCTAAACAAG
  GATGGGAAGATGGATCAGCAAGAGTTCTCCATAGCTATGAAACTCATCAAACTGAAGCTTCAAGGCCAAC
  AGTTGCCTGTGGTTCTCCCTCCTATTATGAAGCAACCCCCTATGTTTTCTCCATTAATTTCTGCTCGTTT
  TGGAATGGGAAGCATGCCCAATCTGTCCATTCCTCAGCCATTGCCTCCAGCTGCACCTATAACATCATTG
  TCTTCTGCGACTTCAGGGACCAACCTTCCTCCCTTAATGATGCCCACTCCCCTAGTGCCTTCTGTTAGCA
  CATCATCATTACCAAATGGAACCGCCAGTCTCATTCAGCCTTTACCCATTCCTTATTCTTCTTCAACATT
  GCCTCATGGGTCATCTTATAGTCTGATGATGGGAGGATTTGGAGGTGCTAGTATACAGAAAGCGCAGTCT
  CTGATTGATTTAGGATCTAGTAGCTCAACTTCCTCGACTGCTTCACTCTCAGGGAACTCACCCAAGACTG
  GGACCTCAGAGTGGGCAGTTCCTCAGCCTACAAGATTAAAATATCGGCAAAAATTTAATACTCTTGACAA
  AAGTATGAGTGGATATCTCTCAGGTTTTCAAGCTAGAAATGCCCTTCTTCAGTCAAATCTTTCTCAAACT
  CAGCTGGCTACTATTTGGACTCTGGCTGACGTTGATGGTGATGGACAGCTAAAAGCAGAAGAGTTTATTC
  TTGCAATGCACCTTACTGACATGGCCAAAGCTGGACAGCCATTACCACTGACTTTACCTCCTGAGCTTGT
  TCCTCCATCTTTCAGAGGAGGAAAGCAAATTGATTCCATTAATGGAACTCTGCCTTCATATCAGAAAATG
  CAAGAAGAGGAGCCTCAGAAGAAATTACCAGTTACTTTTGAGGACAAACGGAAAGCCAACTATGAGCGAG
  GGAACATGGAGCTGGAAAAGCGACGCCAAGCCTTGATGGAGCAGCAACAAAGGGAGGCAGAACGTAAAGC
  CCAGAAAGAAAAGGAAGAGTGGGAACGAAAACAGAGAGAATTACAAGAACAAGAATGGAAGAAACAACTT
  GAATTAGAAAAACGCTTAGAGAAGCAACGGGAATTGGAGAGACAACGAGAGGAAGAAAGGAGAAAAGACA
  TAGAAAGACGAGAGGCAGCAAAACAGGAACTTGAACGACAACGTCGCTTAGAATGGGAGAGAATTCGGCG
  ACAGGAGCTTCTCAATCAAAAGAATAGAGAACAAGAAGAAATTGTCAGGTTAAACTCTAAAAAGAAGAAT
  CTTCATCTTGAGTTGGAAGCACTGAATGGCAAACATCAGCAGATCTCAGGCAGACTTCAGGATGTCCGAC
  TCAAAAAGCAAACTCAAAAGACTGAGCTGGAAGTTCTGGATAAGCAGTGTGACTTGGAAATTATGGAAAT
  CAAGCAACTTCAACAGGAACTTCAGGAATATCAGAATAAGCTTATCTATCTGGTACCTGAGAAGCAATTA
  TTAAATGAAAGAATTAAAAACATGCAGTTCAGTAACACACCTGATTCAGGGGTCAGTTTACTTCATAAAA
  AATCATTAGAAAAGGAAGAATTATGCCAAAGACTTAAAGAACAGTTAGATGCTCTTGAAAAAGAAACTGC
  ATCTAAGCTGTCAGAAATGGATTCTTTTAACAATCAACTAAAGTGTGGGAATATGGATGACTCTGTTCTT
  CAGTGCCTTTTGTCTCTGCTAAGCTGTCTCAACAACCTCTTCCTCTTACTTAAGGAACTGAGAGAAACCT
  ACAACACACAGCAGTTAGCCCTTGAACAGCTTTATAAGATCAAACGTGACAAGTTGAAGGAAATTGAAAG
  GAAAAGATTAGAACTAATGCAGAAAAAGAAACTAGAAGATGAGGCTGCAAGGAAAGCAAAGCAAGGAAAA
  GAAAACTTATGGAAAGAAAATCTTAGAAAGGAGGAAGAAGAAAAACAAAAGCGACTCCAGGAAGAAAAAA
  CACAAGAAAAAATTCAAGAAGAGGAACGGAAAGCTGAGGAGAAACAACGTAAGGATAAGGATACTTTGAA
  AGCTGAGGAGAAAAAACGTGAGACAGCTAGTGTTTTGGTGAATTATAGAGCATTATACCCCTTTGAAGCA
  AGGAACCATGATGAGATGAGTTTTAATTCTGGAGATATAATTCAGGTTGATGAAAAAACCGTAGGAGAAC
  CTGGTTGGCTTTATGGTAGTTTTCAAGGAAATTTTGGCTGGTTTCCATGCAATTATGTAGAAAAAATGCC
  ATCAAGTGAAAATGAAAAAGCTGTATCTCCAAAGAAGGCCTTACTTCCTCCTACAGTTTCTTTATCTGCT
  ACCTCAACTTCCTCTGAACCACTTTCTTCAAATCAACCAGCATCAGTGACTGATTATCAAAATGTATCTT
  TTTCAAACCTAACTGTAAATACATCATGGCAGAAAAAATCAGCCTTCACTCGAACTGTGTCCCCTGGATC
  TGTATCACCTATTCATGGACAGGGACAAGTGGTAGAAAACTTAAAAGCACAGGCCCTTTGTTCCTGGACT
  GCAAAGAAAGATAACCACTTGAACTTCTCAAAACATGACATTATTACTGTCTTGGAGCAGCAAGAAAATT
  GGTGGTTTGGGGAGGTGCATGGAGGAAGAGGATGGTTTCCCAAATCTTATGTCAAGATCATTCCTGGGAG
  TGAAGTAAAACGGGAAGAACCAGAAGCTTTGTATGCAGCTGTAAATAAGAAACCTACCTCGGCAGCCTAT
  TCAGTTGGAGAAGAATATATTGCACTTTATCCATATTCAAGTGTGGAACCTGGAGATTTGACTTTCACAG
  AAGGTGAAGAAATATTGGTGACCCAGAAAGATGGAGAGTGGTGGACAGGAAGTATTGGAGATAGAAGTGG
  AATTTTTCCATCAAACTATGTCAAACCAAAGGATCAAGAGAGTTTTGGGAGTGCTAGCAAGTCTGGAGCA
  TCAAATAAAAAACCTGAGATTGCTCAGGTAACTTCAGCATATGTTGCTTCTGGTTCTGAACAACTTAGCC
  TTGCACCAGGACAGTTAATATTAATTCTAAAGAAAAATACAAGTGGGTGGTGGCAAGGAGAGTTACAGGC
  CAGAGGAAAAAAGCGACAGAAAGGATGGTTTCCTGCCAGTCATGTTAAACTTTTGGGTCCAAGTAGTGAA
  AGAGCCACACCTGCCTTTCATCCTGTATGTCAGGTGATTGCTATGTATGACTATGCAGCAAATAATGAAG
  ATGAGCTCAGTTTCTCCAAGGGACAACTCATTAATGTTATGAACAAAGATGATCCTGATTGGTGGCAAGG
  AGAGATCAACGGGGTGACTGGTCTCTTTCCTTCAAACTACGTTAAGATGACGACAGACTCAGATCCAAGT
  CAACAGTGGTGTGCTGATCTGCAAACCCTGGACACAATGCAGCCAATTGAGAGGAAAAGACAGGGCTATA
  TTCATGAGCTGATTCAGACCGAAGAGCGGTACATGGCTGACCTTCAGCTCGTCGTCGAGGTTTTTCAGAA
  ACGCATGGCAGAGTCAGGCTTTCTCACTGAAGGGGAGATGGCCCTGATTTTTGTTAACTGGAAGGAGCTC
  ATCATGTCCAACACAAAGCTGCTGAAGGCTTTGCGGGTGCGGAAGAAGACCGGGGGCGAGAAGATGCCGG
  TGCAGATGATTGGGGACATCCTGGCCGCTGAGCTGTCCCACATGCAGGCTTACATCAGGTTCTGCAGCTG
  CCAGCTTAATGGAGCAGCTCTGTTACAGCAGAAGACAGATGAAGACACAGATTTCAAAGAATTTTTAAAG
  AAGCTGGCATCTGACCCGCGGTGTAAAGGAATGCCCCTCTCCAGCTTCCTGCTGAAACCCATGCAGAGGA
  TCACCCGCTACCCACTGCTCATCAGAAGTATTCTGGAGAACACCCCGGAGAGCCATGCAGACCATTCCTC
  CCTAAAGCTGGCCCTCGAGCGGGCAGAGGAGCTGTGCTCTCAAGTGAATGAGGGAGTTCGGGAGAAGGAA
  AACTCGGACCGACTGGAGTGGATCCAGGCGCACGTGCAGTGTGAAGGCCTCGCGGAGCAACTTATTTTCA
  ACTCTCTCACCAACTGCCTGGGGCCCCGGAAGCTCTTACACAGTGGGAAATTATACAAGACCAAGAGCAA
  CAAGGAACTGCACGGATTCCTCTTCAATGACTTCCTGCTTCTTACCTACATGGTCAAGCAGTTTGCTGTT
  TCCTCTGGCTCTGAGAAACTTTTCAGCTCGAAGTCCAATGCTCAATTCAAAATGTATAAAACGCCCATTT
  TCCTGAATGAAGTCTTGGTGAAACTGCCCACAGACCCTTCCAGCGATGAGCCTGTCTTCCACATTTCCCA
  CATTGATCGGGTCTACACCCTCCGAACAGACAACATTAATGAGAGGACCGCCTGGGTGCAGAAGATCAAG
  GCGGCGTCTGAGCAGTACATCGACACCGAGAAGAAGAAGCGTGAGAAAGCTTACCAAGCCCGCTCCCAAA
  AGACTTCAGGCATTGGGCGCCTGATGGTGCATGTCATTGAAGCTACAGAATTAAAAGCCTGCAAACCAAA
  TGGAAAGAGCAACCCATACTGTGAAATCAGCATGGGCTCCCAGAGCTACACCACCAGGACCATCCAGGAC
  ACACTCAATCCCAAGTGGAATTTTAACTGCCAGTTCTTTATTAAGGATCTCTACCAAGACGTGCTGTGTC
  TCACCCTGTTTGACAGAGACCAGTTTTCACCAGATGATTTCCTGGGTCGTACTGAAATTCCAGTGGCAAA
  AATTCGAACAGAACAGGAAAGCAAAGGCCCTATGACCCGCCGACTGCTGCTGCATGAGGTCCCCACCGGG
  GAGGTCTGGGTCCGTTTTGACCTGCAGCTTTTTGAGCAAAAAACTCTCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229449 representing NM_006277
  Red=Cloning site Green=Tags(s)
  
  MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNK
  DGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSL
  SSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQS
  LIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQT
  QLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKM
  QEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQL
  ELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKN
  LHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQL
  LNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVL
  QCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGK
  ENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEA
  RNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSA
  TSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWT
  AKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAY
  SVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGA
  SNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSE
  RATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPS
  QQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKEL
  IMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLK
  KLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKE
  NSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAV
  SSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIK
  AASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQD
  TLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTG
  EVWVRFDLQLFEQKTLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006277
• ORF Size: 5091 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006277.3
• RefSeq ORF: 5094 bp
• Locus ID: 50618
• UniProt ID: Q9NZM3
• Cytogenetics: 2p23.3
• Protein Families: Druggable Genome
• MW: 193.3 kDa
• Gene Summary: This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. [provided by RefSeq, Jan 2017]