Intersectin 2 (ITSN2) Rabbit Polyclonal Antibody

CAT#: TA377634

ITSN2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ITSN2 antibody (TA377634) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunofluorescence analysis of C6 cells using ITSN2 antibody (TA377634) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of L929 cells using ITSN2 antibody (TA377634) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using ITSN2 antibody (TA377634) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 500-620 of human ITSN2 (NP_006268.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 135kDa/141kDa/190kDa/193kDa
• Gene Name: intersectin 2
• Database Link:
  Entrez Gene 50618 Human
  UniProt ID Q9NZM3
• Background: This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis.
• Synonyms: KIAA1256; OTTHUMP00000116039; PRO2015; SH3D1B; SH3P18; SWA; SWAP