WRCH1 (RHOU) (NM_021205) Human Untagged Clone

CAT#: SC112928

RHOU (untagged)-Human ras homolog gene family, member U (RHOU), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WRCH1
• Synonyms: ARHU; CDC42L1; G28K; hG28K; WRCH1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112928 sequence for NM_021205 edited (data generated by NextGen Sequencing)
  ATGCCCCCGCAGCAGGGGGACCCCGCGTTCCCCGACCGCTGCGAGGCGCCTCCGGTGCCG
  CCGCGTCGGGAGCGCGGTGGACGCGGGGGACGCGGGCCTGGGGAGCCGGGGGGCCGGGGG
  CGTGCGGGGGGTGCCGAGGGGCGCGGCGTCAAGTGCGTGCTGGTCGGCGACGGCGCGGTG
  GGCAAGACGAGCCTGGTGGTGAGCTACACCACCAACGGCTACCCCACCGAGTACATCCCT
  ACTGCCTTCGACAACTTCTCCGCGGTGGTGTCTGTGGATGGGCGGCCCGTGAGACTCCAA
  CTCTGTGACACTGCCGGACAGGATGAATTTGACAAGCTGAGGCCTCTCTGCTACACCAAC
  ACAGACATCTTCCTGCTCTGCTTCAGTGTCGTGAGCCCCTCATCCTTCCAGAACGTCAGT
  GAGAAATGGGTGCCGGAGATTCGATGCCACTGTCCCAAAGCCCCCATCATCCTAGTTGGA
  ACGCAGTCGGATCTCAGAGAAGATGTCAAAGTCCTCATTGAGTTGGACAAATGCAAAGAA
  AAGCCAGTGCCTGAAGAGGCGGCTAAGCTGTGCGCCGAGGAAATCAAAGCCGCCTCCTAC
  ATCGAGTGTTCAGCCTTGACTCAAAAAAACCTCAAAGAGGTCTTTGATGCAGCCATCGTC
  GCTGGCATTCAATACTCGGACACTCAGCAACAGCCAAAGAAGTCTAAAAGCAGGACTCCA
  GATAAAATGAAAAACCTCTCCAAGTCCTGGTGGAAGAAGTACTGCTGTTTCGTATGA
  
  Clone variation with respect to NM_021205.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021205 unedited
  CGGCCGGCGAATTCGCACGAGGGCGCTCGGACCGCTGTCCTCCAACAGCGCAGGGCAGAG
  CGGCTGGCGCCGCCGGAGCGCGGAGCCACGACCCTCCCTGGCCGCCTTTGTCTACTGGCC
  GTGCGGCCCGGAACCGCCACTCTCCAGGGCCGGGGACGCGCCCGCAGCTGTCGGTGACAG
  CTCCTCCCTACCGCAACCCTCCGGGGCGGAGGGGCGGTCGGGCCGGGCCCTGCTAGCCCG
  CGACCGCAAGCCCGCGCTCGCGGATCGATGCCCCCGCAGCAGGGGGACCCCGCGTTCCCC
  GACCGCTGCGAGGCGCCTCCGGTGCCGCCGCGTCGGGAGCGCGGTGGACGCGGGGGACGC
  GGGCCTGGGGAGCCGGGGGGCCGGGGGCGTGCGGGGGGTGCCGAGGGGCGCGGCGTCAAG
  TGCGTGCTGGTCGGCGACGGCGCGGTGGGCAAGACGAGCCTGGTGGTGAGCTACACCACC
  AACGGCTACCCCACCGAGTACATCCCTACTGCCTTCGACAACTTCTCCGCGGGTGGTGTC
  TGTGGATGGGGCGGGCCCGTGAGACTCCAACTCTGTGACACTGCCGGACAGGATGAATNT
  GACAAGCTGAGGCCTCTCTGCTACACCAACACAGACATCTTCCTGCTCTGCTTCAGTGGT
  CGTGAGCCCCTCATCCTTCCAGAACGTCAGTGAGAAAATGGGTGCCGGGAGATTCGATGC
  CACCTGTCCCAAAAGCCCCCATCATCCTAGNTGGNAAACGCAGTCGGATCTCANAAAAAG
  AGGTCAAAGTCCTCATTGGAGTTGGACAAATGCAAAGAAAAGCCAGTGCCTGGAGAGGCN
  NGCTAATCTGTGCGCCGAAGAAATCAAAGGCGCCTCTACAACCAGTGGTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021205 unedited
  ATGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATAAGTCAGAA
  TTTATTTCATACCATCTCACTTATAGCATTTTCAAGTACAACATTCTGCTCAACATCATT
  TACACTTGAAAACAGAAAAGCACAACTTGGTAAGGCACCAGGTTACGATAGTCTGGAGAG
  AAGGCCTTGCTCCCATTTTGGCTTGTGTAATACCTGGGTAGTTTCTCTTGAGTCTGTCAA
  GCAGAGAACAAGGTTATAAAAGGTCCATTTATACATACATGGTAACAAGAGATAACAAAC
  AGTTTTGAAGTATGCTGTATTTATAAATTATAATGGTGGCCTACACTTGTAGTTCAGCCA
  AAGTGGCATTCTCTAAAGCAAAATTCTTATAAAATCTTCTCTGCAATACCAAGCTGCAAG
  TTTAACAATTTTTTAGCTTTGAAGTGAACCAACTTTATATTTAACTCAAACACATACTTT
  AAAAACATTTTCGGCCCCAAACTCTATGTTCACGAAGAAATAAAAATGGAGGAAAATCTC
  AAGTATAATCGTACCTTTACTATTCTATAAAATGCAGCAATATATTTTAAAAACTGTTGC
  ATTTAAAGGTCAACCCACTAGATTTTTAACACTAACAGTCCAAGGTGTTATCAACCCCAC
  TTAAAATTCAACTGTATAAAGCTTTCAGAACTGGGCCACACAGGTGTACTTCACTGAGCA
  CTTTCCACCCATGGCCTAAAGGCAGGAGGTGTGGAAGTCCCCTCCAGGGAGACCAACTGC
  AGCCCCATACACTGTCACACGCATAAAACCTCCTTTAAGTTTTGTGTTCTATCTCAAAAT
  GAAATTTGCCTATACTCAATGCCATTTACTTTATATAAAAAAGTGGTTCAAACCGCTACA
  GTTAAATACTTATCTTTCCTCCACACTCCAGCCCCAGAAGGTAGANCCAGTCTGTAAGCT
  ANCTTCCCAAAAGGTACCCAGCCCGCAGAAGACCGTTTTAGTAGGCATTTTAC
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_021205
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021205.4, NP_067028.1
• RefSeq Size: 4369 bp
• RefSeq ORF: 777 bp
• Locus ID: 58480
• UniProt ID: Q7L0Q8
• Cytogenetics: 1q42.13
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. A non-coding transcript variant of this gene results from naturally occurring read-through transcription between this locus and the neighboring DUSP5P (dual specificity phosphatase 5 pseudogene) locus.[provided by RefSeq, Mar 2011]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein.