WRCH1 (RHOU) (NM_021205) Human Tagged ORF Clone
CAT#: RC207871
RHOU (Myc-DDK-tagged)-Human ras homolog gene family, member U (RHOU), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_021205" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | WRCH1 |
Synonyms | ARHU; CDC42L1; G28K; hG28K; WRCH1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207871 representing NM_021205
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCCGCAGCAGGGGGACCCCGCGTTCCCCGACCGCTGCGAGGCGCCTCCGGTGCCGCCGCGTCGGG AGCGCGGTGGACGCGGGGGACGCGGGCCTGGGGAGCCGGGGGGCCGGGGGCGTGCGGGGGGTGCCGAGGG GCGCGGCGTCAAGTGCGTGCTGGTCGGCGACGGCGCGGTGGGCAAGACGAGCCTGGTGGTGAGCTACACC ACCAACGGCTACCCCACCGAGTACATCCCTACTGCCTTCGACAACTTCTCCGCGGTGGTGTCTGTGGATG GGCGGCCCGTGAGACTCCAACTCTGTGACACTGCCGGACAGGATGAATTTGACAAGCTGAGGCCTCTCTG CTACACCAACACAGACATCTTCCTGCTCTGCTTCAGTGTCGTGAGCCCCTCATCCTTCCAGAACGTCAGT GAGAAATGGGTGCCGGAGATTCGATGCCACTGTCCCAAAGCCCCCATCATCCTAGTTGGAACGCAGTCGG ATCTCAGAGAAGATGTCAAAGTCCTCATTGAGTTGGACAAATGCAAAGAAAAGCCAGTGCCTGAAGAGGC GGCTAAGCTGTGCGCCGAGGAAATCAAAGCCGCCTCCTACATCGAGTGTTCAGCCTTGACTCAAAAAAAC CTCAAAGAGGTCTTTGATGCAGCCATCGTCGCTGGCATTCAATACTCGGACACTCAGCAACAGCCAAAGA AGTCTAAAAGCAGGACTCCAGATAAAATGAAAAACCTCTCCAAGTCCTGGTGGAAGAAGTACTGCTGTTT CGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207871 representing NM_021205
Red=Cloning site Green=Tags(s) MPPQQGDPAFPDRCEAPPVPPRRERGGRGGRGPGEPGGRGRAGGAEGRGVKCVLVGDGAVGKTSLVVSYT TNGYPTEYIPTAFDNFSAVVSVDGRPVRLQLCDTAGQDEFDKLRPLCYTNTDIFLLCFSVVSPSSFQNVS EKWVPEIRCHCPKAPIILVGTQSDLREDVKVLIELDKCKEKPVPEEAAKLCAEEIKAASYIECSALTQKN LKEVFDAAIVAGIQYSDTQQQPKKSKSRTPDKMKNLSKSWWKKYCCFV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021205 |
ORF Size | 774 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021205.6 |
RefSeq Size | 4369 bp |
RefSeq ORF | 777 bp |
Locus ID | 58480 |
UniProt ID | Q7L0Q8 |
Cytogenetics | 1q42.13 |
Domains | ras, RAN, RAS, RHO, RAB |
Protein Families | Druggable Genome |
MW | 28 kDa |
Gene Summary | This gene encodes a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. A non-coding transcript variant of this gene results from naturally occurring read-through transcription between this locus and the neighboring DUSP5P (dual specificity phosphatase 5 pseudogene) locus.[provided by RefSeq, Mar 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207871L1 | Lenti ORF clone of Human ras homolog gene family, member U (RHOU), transcript variant 1, Myc-DDK-tagged |
USD 600.00 |
|
RC207871L2 | Lenti ORF clone of Human ras homolog gene family, member U (RHOU), transcript variant 1, mGFP tagged |
USD 600.00 |
|
RC207871L3 | Lenti ORF clone of Human ras homolog gene family, member U (RHOU), transcript variant 1, Myc-DDK-tagged |
USD 600.00 |
|
RC207871L4 | Lenti ORF clone of Human ras homolog gene family, member U (RHOU), transcript variant 1, mGFP tagged |
USD 600.00 |
|
RG207871 | RHOU (tGFP-tagged) - Human ras homolog gene family, member U (RHOU), transcript variant 1 |
USD 500.00 |
|
SC112928 | RHOU (untagged)-Human ras homolog gene family, member U (RHOU), transcript variant 1 |
USD 300.00 |
|
SC323988 | RHOU (untagged)-Human ras homolog gene family, member U (RHOU), transcript variant 1 |
USD 300.00 |
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