LEPRE1 (P3H1) (NM_022356) Human Untagged Clone

CAT#: SC112534

LEPRE1 (untagged)-Human leucine proline-enriched proteoglycan (leprecan) 1 (LEPRE1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LEPRE1
• Synonyms: GROS1; LEPRE1; OI8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_022356 edited
  ATGGCGGTACGCGCGTTGAAGCTGCTGACCACACTGCTGGCTGTCGTGGCCGCTGCCTCC
  CAAGCCGAGGTCGAGTCCGAGGCAGGATGGGGCATGGTGACGCCTGATCTGCTCTTCGCC
  GAGGGGACCGCAGCCTACGCGCGCGGGGACTGGCCCGGGGTGGTCCTGAGCATGGAACGG
  GCGCTGCGCTCCCGGGCAGCCCTCCGCGCCCTTCGCCTGCGCTGCCGCACCCAGTGTGCC
  GCCGACTTCCCGTGGGAGCTGGACCCCGACTGGTCCCCCAGCCCGGCCCAGGCCTCGGGC
  GCCGCCGCCCTGCGCGACCTGAGCTTCTTCGGGGGCCTTCTGCGTCGCGCTGCCTGCCTG
  CGCCGCTGCCTCGGGCCGCCGGCCGCCCACTCGCTCAGCGAAGAGATGGAGCTGGAGTTC
  CGCAAGCGGAGCCCCTACAACTACCTGCAGGTCGCCTACTTCAAGATCAACAAGTTGGAG
  AAAGCTGTTGCTGCAGCACACACCTTCTTCGTGGGCAATCCTGAGCACATGGAAATGCAG
  CAGAACCTAGACTATTACCAAACCATGTCTGGAGTGAAGGAGGCCGACTTCAAGGATCTT
  GAGACTCAACCCCATATGCAAGAATTTCGACTGGGAGTGCGACTCTACTCAGAGGAACAG
  CCACAGGAAGCTGTGCCCCACCTAGAGGCGGCGCTGCAAGAATACTTTGTGGCCTATGAG
  GAGTGCCGTGCCCTCTGCGAAGGGCCCTATGACTACGATGGCTACAACTACCTTGAGTAC
  AACGCTGACCTCTTCCAGGCCATCACAGATCATTACATCCAGGTCCTCAACTGTAAGCAG
  AACTGTGTCACGGAGCTTGCTTCCCACCCAAGTCGAGAGAAGCCCTTTGAAGACTTCCTC
  CCATCGCATTATAATTATCTGCAGTTTGCCTACTATAACATTGGGAATTATACACAGGCT
  GTTGAATGTGCCAAGACCTATCTTCTCTTCTTCCCCAATGACGAGGTGATGAACCAAAAT
  TTGGCCTATTATGCAGCTATGCTTGGAGAAGAACACACCAGATCCATCGGCCCCCGTGAG
  AGTGCCAAGGAGTACCGACAGCGAAGCCTACTGGAAAAAGAACTGCTTTTCTTCGCTTAT
  GATGTTTTTGGAATTCCCTTTGTGGATCCGGATTCATGGACTCCAGAAGAAGTGATTCCC
  AAGAGATTGCAAGAGAAACAGAAGTCAGAACGGGAAACAGCCGTACGCATCTCCCAGGAG
  ATTGGGAACCTTATGAAGGAAATCGAGACCCTTGTGGAAGAGAAGACCAAGGAGTCACTG
  GATGTGAGCAGACTGACCCGGGAAGGTGGCCCCCTGCTGTATGAAGGCATCAGTCTCACC
  ATGAACTCCAAACTCCTGAATGGTTCCCAGCGGGTGGTGATGGACGGCGTAATCTCTGAC
  CACGAGTGTCAGGAGCTGCAGAGACTGACCAATGTGGCAGCAACCTCAGGAGATGGCTAC
  CGGGGTCAGACCTCCCCACATACTCCCAATGAAAAGTTCTATGGTGTCACTGTCTTCAAA
  GCCCTCAAGCTGGGGCAAGAAGGCAAAGTTCCTCTGCAGAGTGCCCACCTGTACTACAAC
  GTGACGGAGAAGGTGCGGCGCATCATAGAGTCCTACTTCCGCCTGGATACGCCCCTCTAC
  TTTTCCTACTCTCATCTGGTGTGCCGCACTGCCATCGAAGAGGTCCAGGCAGAGAGGAAG
  GATGATAGTCATCCAGTCCACGTGGACAACTGCATCCTGAATGCCGAGACCCTCGTGTGT
  GTCAAAGAGCCCCCAGCCTACACCTTCCGCGACTACAGCGCCATCCTTTACCTAAATGGG
  GACTTCGATGGCGGAAACTTTTATTTCACTGAACTGGATGCCAAGACCGTGACGGCAGAG
  GTGCAGCCTCAGTGTGGAAGAGCCGTGGGATTCTCTTCAGGCACTGAAAACCCACATGGA
  GTGAAGGCTGTCACCAGGGGGCAGCGCTGTGCCATCGCCCTGTGGTTCACCCTGGACCCT
  CGACACAGCGAGCGGGACAGGGTGCAGGCAGATGACCTGGTGAAGATGCTCTTCAGCCCA
  GAAGAGATGGACCTCTCCCAGGAGCAGCCCCTGGATGCCCAGCAGGGCCCCCCCGAACCT
  GCACAAGAGTCTCTCTCAGGCAGTGAATCGAAGCCCAAGGATGAGCTATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022356 unedited
  GGTTCGAAATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGGTGG
  CTGGCGGTTCCGTTAGGTCTGAGGGAGCGATGGCGGTACGCGCGTTGAAGCTGCTGACCA
  CACTGCTGGCTGTCGTGGCCGCTGCCTCCCAAGCCGAGGTCGAGTCCGAGGCAGGATGGG
  GCATGGTGACGCCTGATCTGCTCTTCGCCGAGGGGACCGCAGCCTACGCGCGCGGGGACT
  GGCCCGGGGTGGTCCTGAGCATGGAACGGGCGCTGCGCTCCCGGGCAGCCCTCCGCGCCC
  TTCGCCTGCGCTGCCGCACCCAGTGTGCCGCCGACTTCCCGTGGGAGCTGGACCCCGACT
  GGTCCCCCAGCCCGGCCCAGGCCTCGGGCGCCGCCGCCCTGCGCGACCTGAGCTTCTTCG
  GGGGCCTTCTGCGTCGCGCTGCCTGCCTGCGCCGCTGCCTCGGGCCGCCGGCCGCCCACT
  CGCTCAGCGAAGAGATGGAGCTGGAGTTCCGCAAGCGGAGCCCCTACAACTACCTGCAGG
  TCGCCTACTTCAAGATCAACAAGTTGGAGAAAGCTGTTGCTGCAGCACACACCTTCTTCG
  TGGGCAATCCTGAGCACATGGAAATGCAGCAGAACCTAGACTATTACCAAACCATGTCTG
  GAGTGAAGGAGGCCGACTTCAAGGATCTTGAGACTCAACCCCATATGCAAGAATTTCGAC
  TGGGAGTGCGACTCTACTCAGAGGAACAGCCACCAGAAGCTGTGCCCCACCTAGAGGCCG
  CGCTGCAAGAATACTTTGTGGCCTATGAGGAGTGCCGTGCCCTCTGCGAAAGCCCCTATG
  ACTACGATGGCTACCACTACCTTGGATACAACGCTGACCTCTTCCAGGCCATCACAGATC
  ATTACATCCAAGTTCTCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022356 unedited
  NTTTACCTGGATNATTTATTCTTTGGTTGTCTACACAGACACTTAAGTACTGTATCGCTG
  TTATGCAGCGGCCTGTGGAGGCCCCTGGGGGTGGCTGGGCCTGTGTCCTGAGCCCTCAGC
  CAGATCCAGGGGGTGCGGTGTCTGGTCATGTCCACTCCAAGAGCAGTAGCACCATGTAGA
  AGGCTGTGAGCAGGGTCCCCTCGGCTGACTGGCAGATGTAGGCTCACTGCTCTGCAGCCC
  CGAGGGGCTGGCCAGCTCAGAGTGCAGAAGAGTTCCTCTCCATGGGTCTAGTCACCCATC
  CGTCTGACCTGGACGCTGTCATAGCTCATCCTTGGGCTTCGATTCACTGGCTGAGAGAGA
  CTGTTGTGCAGGTACTGGGGGCCCCTGCTGGGCATCCAGGGGCTGCTCCTGCGATAGGTG
  CATAAATTGTGGGCTGTGCACCATCTTCACCGGCTCATATAGCTGCTCCCTGTATCACTC
  CTTGGGCTAAGGTATCCACCTAAACACCACGACGCTGGTGCGACATTGTCTGTGCTGTCA
  ATATAATCAAATTAATGCATCCATTGTTAGATTTAAGATTTATCTCTTTTTTTCGTCCGT
  CTTATCTGTACTTTTATCTTTTCTTCTGTCTCTTCCCTTTCCTCGATCCCCCCCCTAGTC
  CCCTCTTCCTCCTTCCTCCTTATATTACTCCCCTCCCGCTCCTTCGCACTTTCCAACACC
  TCGGCTTCGATAATACTCCGTTCTCTCCTCCTTCGTCCTCTCCCTCACCCACCGCATATC
  CACCCGATTCCCCCCTTCCGCGTTTCCCGCCTGCTCTCCTTTCCCCTCTCTCCTTATTCT
  CCCTCCCTCTCTCGCGGCTTCCCCTCCCTGTTTCTCTTTTTTCCTCGCCCTCTTCTCCCT
  CCTCGCTTTCTGTATCTCTCTCCCCCTCTCTCCTTCTCCGTCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022356
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022356.2, NP_071751.2
• RefSeq Size: 2993 bp
• RefSeq ORF: 2415 bp
• Locus ID: 64175
• UniProt ID: Q32P28
• Cytogenetics: 1p34.2
• Domains: 2OG-FeII_Oxy, P4Hc
• Protein Families: Secreted Protein
• Gene Summary: This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Three alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (1) differs in the 3' UTR and coding sequence compared to variant 3. The resulting isoform (1) has a shorter and distinct C-terminus compared to isoform 3.