FRMD8 (NM_031904) Human Untagged Clone

CAT#: SC107202

FRMD8 (untagged)-Human FERM domain containing 8 (FRMD8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FRMD8
• Synonyms: FKSG44; iTAP
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107202 sequence for NM_031904 edited (data generated by NextGen Sequencing)
  ATGGACGGGACAGAAGGCAGTGCCGGGCAGCCCGGCCCCGCTGAGCGATCCCACCGAAGC
  AGCGTGTCCTCCGTGGGAGCCCGAGCGGCTGACGTGCTGGTATACCTAGCGGATGACACG
  GTGGTGCCCCTGGCTGTGGAGAACCTGCCCTCGCTCAGTGCCCATGAGCTGCACCGCGCT
  GTCCGCGAGGTCCTGCAGCTTCCAGACATCGCCCTGGATGTCTTCGCGCTCTGGCTGGTC
  TCCCCTCTGCTGGAGGTGCAGCTGAAACCCAAGCACCAGCCCTACAAGCTGGGACGCCAG
  TGGCCGGAGCTGCTGCTGCGCTTCACCAGTGCCCCAGATGATGACGTGGCCATGGATGAG
  CCTTTCCTGCAGTTCCGAAGGAACGTGTTCTTCCCAAAGCGGCGGGAGCTCCAGATCCAT
  GACGAGGAGGTCCTGCGGCTGCTCTATGAGGAGGCCAAGGGCAACGTGCTGGCTGCACGG
  TACCCGTGCGACGTGGAGGACTGCGAGGCTCTGGGCGCCCTGGTGTGCCGCGTGCAGCTT
  GGGCCCTACCAGCCCGGCCGGCCGGCAGCCTGCGACCTGAGGGAGAAGCTGGACTCCTTC
  CTCCCTGCCCACCTCTGTAAGCGGGGCCAGAGTCTCTTTGCTGCCCTCCGGGGCCGTGGG
  GCCAGGGCCGGGCCGGGCGAGCAGGGCCTGCTGAACGCCTACCGCCAGGTGCAGGAGGTC
  AGCAGCGACGGCGGGTGCGAGGCCGCCCTGGGCACCCACTACCGCGCCTATCTCCTCAAG
  TGCCACGAGCTGCCGTTTTATGGGTGTGCCTTCTTCCACGGTGAGGTTGACAAGCCGGCC
  CAAGGCTTTTTGCACCGGGGTGGGCGCAAGCCAGTTTCTGTGGCCATCAGTCTGGAAGGC
  GTGCACGTCATCGATAGCAGAGAGAAGCATGTCCTGCTGGGCCTGCGCTTCCAGGAGCTG
  TCGTGGGACCACACCTCCCCCGAGGAGGAGGAGCCCATCTTGTGGCTGGAGTTCGACGGG
  GACAGCGAGGGCACACCTGTCAACAAGCTCCTCAAGATCTACTCCAAGCAGGCCGAACTG
  ATGAGCAGTCTCATTGAGTACTGCATCGAACTGAGCCAGGTGGCGGAGCCCGCAGGCCCC
  CAGGACAGTGCGACTGGCTCGCCCTCGGACCCCAGCTCCTCACTGGCTCCTGTTCAGCGC
  CCCAAGCTGCGGAGGCAGGGCAGTGTGGTGTCCAGCCGGATCCAGCATCTCTCCACCATC
  GACTACGTGGAGGACGGCAAGGGGATCAGGCGAGTGAAGCCGAAGCGCACCACATCCTTC
  TTCAGCCGGCAGCTGTCCTTGGGCCAGGGGAGCTACACCGTGGTGCAGCCCGGCGACAGC
  CTGGAGCAGGGCTGA
  
  Clone variation with respect to NM_031904.3
  339 c=>t;1121 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031904 unedited
  TCCCCCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA
  GAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA
  ATTCGGCACGAGGGCTGCGTCCTTAGCGGGAGCCCGAGTGCGGGCGGTGGCGGGCTTGGC
  GGCGGGGCAGGATTCCAGGCAGGAGCCTTGCCTCTCAGGTGGCGGGCTCTGCGACCCTGC
  GAGATGGACGGGACAGAAGGCAGTGCCGGGCAGCCCGGCCCCGCTGAGCGATCCCACCGA
  AGCAGCGTGTCCTCCGTGGGAGCCCGAGCGGCTGACGTGCTGGTATACCTAGCGGATGAC
  ACGGTGGTGCCCCTGGCTGTGGAGAACCTGCCCTCGCTCAGTGCCCATGAGCTGCACCGC
  GCTGTCCGCGAGGTCCTGCAGCTTCCAGACATCGCCCTGGATGTCTTCGCGCTCTGGCTG
  GTCTCCCCTCTGCTGGAGGTGCAGCTGAAACCCAAGCACCAGCCCTACAAGCTGGGACGC
  CAGTGGCCGGAGCTGCTGCTGCGCTTCACCAGTGCCCCAGATGATGACGTGGCCATGGAT
  GAGCCTTTCCTGCAGTTCCGAAGGAACGTGTTCTTCCCAAAGCGGCGGGAGCTCCAGATC
  CATGACGAGGAGGTCCTGCGGCTGCTCTATGAGGAGGCCAAGGGCAACGTGCTGGCTGCA
  CGGTACCCGTGCGACGTGGAGGACTGCGAGGCTCTGGGCGCCCTGGTGTGCCGCGTGCAG
  CTTGGGCCCCTACAGCCGGCCGGGCCGCAGCCTGCGACCTGAGGGAGAAGCTGGNACTCT
  TNCTCCCTGCCCACCTCTGTTAGCGGGGCCAGAGTCTCTTTGCTGCCCCTCGGGGCCGTG
  GGCCAGGGCGGGCCCGGCGGCAGGGCCTGCTGAACGCTACCGCCAAGTGCAGAGTCAGCA
  CGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031904 unedited
  TTTAAAACCCTTTNNTTTTTGTTAGAAACTGTTTTTAATAAGTCACATGATACAAAAGAA
  TGAGAACATTCAAAGAATGAGTAAAATACTGCTTTGTCCCAAAGGACAAGCAGAAAATGT
  TAAGGCACAACGGATGCTCAGAAAACGTAAGCAGCTGAAGGGAAAACACATCATCTGTGT
  TCTTCTTACACACACACTCCAACCCATCACACGAACACACCCTCGCCCGCCCATCAGAGA
  AGAATTCGCCTGGAATCAGCTGGGGGCGGTGGCTCACGCCTATAATCCCAGCACTTTGGG
  AGGTTGAGGCGGGCAGATCATGAGGTCAGGAGTTCACGACCAGCCTGACCAACATGGTGA
  AACCCTATCTCTACTAAAAATACAAAAATCAGCGGGGCCTGGTGGCATGCACCTGTAATC
  CCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAGACAGAGGTTGCAGTGAGCCGA
  GATGCGCCACTGCACTCCTGCCTGGGCAACAGAGCAAGACTCCGTCTCAAAAAAAAATAA
  AAAAATAAAAAAAATAAAATCACCTGGAATCAAAGCAGCAGGGACAGCCTGCGCTGGAGC
  CGCCAGAACAGGCAGGCGGGCAGCTCCGCAAGACCGAGCAACTCCAGNACGGGAGACGAG
  GACTGGCAGCTGACATGACCCCTGTGACCAGGACATTTCCAAAGACTGGGGGGCCCCTCC
  CCAAGCCTCTTTCTTTCCGCCCATTATTTTAACAAAATTCCATGCCTGGCCAGATGGCAA
  GCCCTACCCTCGGAAACACCTGGGCCCCATTTCCCAAGCCTACCAAGAGGGAAGGAAGGG
  TGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031904
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031904.2, NP_114110.1
• RefSeq Size: 3693 bp
• RefSeq ORF: 1395 bp
• Locus ID: 83786
• UniProt ID: Q9BZ67
• Cytogenetics: 11q13.1
• Domains: B41
• Gene Summary: Promotes the cell surface stability of iRhom1/RHBDF1 and iRhom2/RHBDF2 and prevents their degradation via the endolysosomal pathway. By acting on iRhoms, involved in ADAM17-mediated shedding of TNF, amphiregulin/AREG, HBEGF and TGFA from the cell surface (PubMed:29897333, PubMed:29897336). Negatively regulates Wnt signaling, possibly by antagonizing the recruitment of AXIN1 to LRP6 (PubMed:19572019).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).