Fbx15 (FBXO15) (NM_152676) Human Untagged Clone

CAT#: SC100626

FBXO15 (untagged)-Human F-box protein 15 (FBXO15), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Fbx15
• Synonyms: FBX15
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100626 sequence for NM_152676 edited (data generated by NextGen Sequencing)
  ATGCCTTCAGAAATCTTGCTGAAGATATTTTCCTACTTGGATGCTGTGAGCCTTCTGTGT
  ACTGGATGTGTGAGCAGGCGCTTTTATCATCTAGCCAATGACAATTTTATTTGGATCGGA
  ATCTACTCAACTGCTTTTTCACCTGCAAGATCAAATTGGAAATTTAATTCAGTAGAGAAG
  ATAGCTATGTCTATGAGCTTTCTGTCAGTTCAGGATAAAGAAGCTGGTTATTGGAAGAAA
  GAATATATCACAAAACAAATAGCATCTGTAAAAGCCGCACTAGCTGACATTCTCAAACCT
  GTCAACCCTTACACAGGCCTTCCAGTTAAGACCAAAGAGGCCCTCAGAATATTTGGTTTA
  GGTTGGGCAATTATACTGAAAGAAAAAGGTGGAAAAGAATATATCATGGAGCATGTTGAT
  CTTTCCATAAATGACACATCAGTTACTGTTATATGGTATGGCAAAAAATGGCCATGCCTA
  GCATCATTGTCAACCTTAGATTTATGTGGCATGACACCAGTTTTTACCGACTGGTATAAA
  ACTCCCACCAAACATAGACTCCGATGGCATTCTTTAATTGCAAAGTACAATCTGAGTCAT
  TTGACCATATCTACCATGATTGGCTGTGACAGACTCATTCGGATCTTCTGCCTGCACCCT
  GGCCTCCTGGTGGGAGTGTGGAAGAAGGAGGAAGAACTGGCTTTTGTTATGGCAAATCTT
  CATTTTCATCACCTTGTGGAGAGGAGCACATTAGGCTCGGCTACTATCCCCTATGAACTG
  CCTCCACATAGCCCCTTTTTGGATGATAGCCCCGAGTATGGACTGCACGGCTACCAACTC
  CATGTTGATCTGCACAGCGGTGGGGTTTTCTACCTATGTGGTACATTTCGCAATCTCTTC
  ACCAAGAGAGGAAATATTGAAAATGGACATGTGAAGCTCATTGTTATACATTTAAAAAAT
  AACAGAGAACACCTACCTCTTATTGGAAAAGTTGGCCTCTCGTGGAAAACTGATATTTTT
  GATGGCTGTATAAAGAGTTGTTCCATGATGGACGTAACTCTTTTGGATGAACATGGGAAA
  CCCTTTTGGTGTTTCAGTTCCCCGGTGTGCCTGAGATCGCCTGCCACACCCTCTGACAGC
  TCTAGCTTCTTGGGACAGACATACAACGTGGACTACGTTGATGCGGAAGGAAGAGTGCAC
  GTGGAGCTGGTGTGGATCAGAGAGACCGAAGAATACCTTATTGTCAACCTGGTCCTTTAT
  CTTAGTATCGCAAAAATCAACCATTGGTTTGGGACTGAATATTAG
  
  Clone variation with respect to NM_152676.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152676 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCCTGTGGTGAAC
  TCCTTGTCTCTATGGCGACTGGACGCGGTCGGATCTTGCAGCAGCACTGGCTCGGCCTCC
  AGACGCTGCGCGGGCCCAGCAGGGGCGGTGGCGCGGCCCGGGGGCGCGCCAGGGCCTTTG
  GGTGCAGAAAGGGGCCAGGGGTCAAGCTTTCTGCAGGCTCTGCTGCCCTGAGGTGCCATG
  CCGGAGGTGGACAGCACTGGGAGAGCTCTTTCTCCTGCTGTTCTGGGTTCCTGGATGGAA
  TGCCTTCAGAAATCTTGCTGAAGATATTTTCCTACTTGGATGCTGTGAGCCTTCTGTGTA
  CTGGATGTGTGAGCAGGCGCTTTTATCATCTAGCCAATGACAATTTTATTTGGATCGGAA
  TCTACTCAACTGCTTTTTCACCTGCAAGATCAAATTGGAAATTTAATTCAGTAGAGAAGA
  TAGCTATGTCTATGAGCTTTCTGTCAGTTCAGGATAAAGAAGCTGGTTATTGGAAGAAAG
  AATATATCACAAAACAAATAGCATCTGTAAAAGCCGCACTAGCTGACATTCTCAAACCTG
  TCAACCCTTACACAGGCCTTCCAGTTAAGACCAAAGAGGCCCTCAGAATATTTGGTTTAG
  GTTGGGCAATTATACTGAAAGAAAAGGTGGAAAAGATATATCATGGAGCATGTTGATCTT
  TCCATAATGACACATCAGTTACTGGTATATGGTATGGCANAAATGGCCATGCCTANCATC
  ATTGGTCACCTTANATTTATGTGGCATGACACCCAGTTTTACCGACTGGTATAANAACTC
  CACCAACATAGACTNCGATGGCATTCTTTTATTGCAAGACANTCTGAGTCTTTGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152676 unedited
  NCGGTTTACTATGNACGCGGCACGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTGCA
  GATTGCTTTATTTAATTTTCAACACCGGGGACAAAGCCAGTCAAAAATAAACAACTAAAT
  AACAACAATAATTTGCCACCTACTGCTAATATTCAGTCCCAAACCAATGGTTGATTTTTG
  CGATACTAAGATAAAGGACCAGGTTGACAATAAGGTATTCTTCGGTCTCTCTGATCCACA
  CCAGGTGGGCGTGCACTCTTCCTTCCGCATCAACGTAGTCCACGTTGTATGTCTGTCCCA
  AGAAGCTAGAGCTGTCAGAAGGTGTGGCAGGCGATCTCAGGCACACCGGGGAACTGAAAC
  ACCAAAAGGGTTTCCCATGTTCATCCAAAAGAGTTACGTCCATCATGGAACAACTCTTTA
  TACAGCCATCAAAAATATCAGTTTTCCACGAGAGGCCAACTTTTCCAATAAGAGGTAGGT
  GTTCTCTGTTATTTTTTAAATGTATAACAATGAGCTTCACATGTCCATTTTCAATATTTC
  CTCTCTTGGTGAAGAGATTGCGAAATGTACCACATAGGTAGAAAACCCCACCGCTGTGCA
  GATCAACATGGAGTTGGTAGCCGTGCAGTCCATACTCCGGGCTATCATCCAAAAAGGGGC
  TATGTGGAAGCAGTTCATANGGGATAATAACCGAACCTAATGTGCTCCTCCTCCCAAGTG
  ATGAAAATGAAGATTTGCCCTAACAAAAGCCAGTGCTTCCTCCTGCTTCACACTCCCACC
  AAGAAGCCCCAGTGGCCGGCCNAAAATCCCCATGGATCTGTCCCACCCCATCCTGGGTAG
  ATATGGGGAAAATGAACTCAAATGGGACCTTTGCAATAAAAAAATGCCTCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152676
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152676.1, NP_689889.1
• RefSeq Size: 1696 bp
• RefSeq ORF: 1305 bp
• Locus ID: 201456
• UniProt ID: Q8NCQ5
• Cytogenetics: 18q22.3
• Domains: F-box
• Protein Families: Druggable Genome
• Gene Summary: Members of the F-box protein family, such as FBXO15, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter protein (isoform 1).