Txnrd3 (NM_001184712) Rat Tagged ORF Clone

CAT#: RR217048

Txnrd3 (myc-DDK-tagged) - Rat thioredoxin reductase 3 (Txnrd3), transcript variant 1

Product Images

Circular map for RR217048

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Txnrd3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217048 representing NM_001184712
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGAGAAGCCACCGTCCCCGCCGCCGCCTCCTCGGGCGCAAACCTCGCCTGGGCAGGGGAAGGCTGGAG
  TCGTCCCCAACCGCCGCCTGGGTGCTGTTCGTGGCGGCCTCATGTCGTCGCCACCCGGCCGCCGCGCCCG
  CCTGACGTCCCCCGGGACCAGCCGCCCGCCCGCTGAGGTCCGCGAGGAGGTGCGGCGCCGCCTGCGGGAT
  CTCATCGAGGGCAACAGGGTTATGATCTTCAGCAAGAGTTACTGTCCACACAGCTCGCGGGTTAAGGAAC
  TCTTTTCATCCTTGGGAGTGAACTATTACATCCTGGAACTTGATCAAGTTGATGATGGGGCCAATGTTCA
  GGAAGTGCTGACAGAAATCAGTAACCAGAAAACGGTGCCCAATATTTTTGTGAATAAAGTGCACGTGGGT
  GGATGTGACCGGATTTTCCAGGCACATCAGAATGGTTTACTGCAGAAGCTCCTTCAGGATGACTCAACTC
  ATGATTATGATCTCATCGTCATCGGCGGGGGCTCTGGCGGCCTCTCTTGTGCCAAGGAAGCTGCCAACTT
  GGGAAAGAAGGTCATGGTGCTAGACTTTGTGGTTCCCTCGCCTCAGGGCACGTCCTGGGGCCTTGGCGGC
  ACCTGTGTGAATGTAGGCTGTATCCCTAAGAAGCTGATGCATCAGGCAGCCCTCCTGGGGCATGCTCTGC
  AAGATGCGAGGAAATATGGCTGGGATTATAACCAGCAGGTGAAGCACAACTGGGAGACCATGAGGGAAGC
  GATCCAGAACCACATTGGCTCCTTGAACTGGGGCTACAGGGTAACTCTTCGGGAGAAAGGCGTGACCTAT
  GTCAACTCCTTCGGGGAGTTTGTGGAACTGCATAAAATAAAGGCAACCAATAAGAAAGGGCAGGAAACTT
  TTTACACCGCTTCGAAGTTTGTCATAGCAACAGGGGAAAGGCCGCGGTACTTGGGAATCCAGGGAGATAA
  AGAGTACTGTATTACCAGCGACGACCTGTTCTCTCTGCCATACTGCCCAGGGAACACATTAATCGTTGGC
  GCCTCGTATGTTGGTCTGGAGTGTGCAGGCTTTTTGGCTGGCTTGGGGCTAGATGTGACAGTCATGGTAC
  GCTCTGTCCTTCTTCGTGGCTTTGATCAAGAAATGGCAGAGAAAGTGGGATCCTACCTGGAACAACAAGG
  CGTCAAGTTCCAAAGGAAATTCACCCCCATTTTGGTTCAACAGTTGGAGAAAGGCTTACCCGGAAGATTG
  AAAGTCGTGGCTAAGTCCACTGAAGGACCAGAGACAGTAGAAGGGACATACAACACGGTTTTGTTAGCAA
  TTGGTCGTGACTCCTGTACAAGGAAAATAGGGCTGGAGAAGATCGGGGTCAAAATCAATGAGAAGAATGG
  CAAAATACCAGTAAATGATGTGGAACAGACCAATGTGCCTCATGTTTATGCTATTGGGGACGTACTGGAG
  GGCAAACCAGAGCTCACACCTGTTGCCATACAGGCAGGCAAGCTGCTAGCTCGAAGACTCTTTGGGATCT
  CTTTAGAAAAGTGTGATTACATTAACGTCCCAACAACGGTGTTCACACCTCTGGAATACGGCTGTTGTGG
  ACTGTCTGAAGAGAAAGCCATTGAAATGTATACGAAGGAGAATCTGGAAGTGTATCACACCTTCTTTTGG
  CCTCTTGAGTGGACAGTTGCTGGCAGAGACAACAACACCTGTTATGCAAAGATAATCTGCAACAAATTTG
  ACAATGACCGTGTGATAGGATTTCACCTCCTGGGGCCAAATGCTGGTGAAGTCACACAGGGGTTTGCAGC
  TGCAATGAAATGTGGGCTTACGAAGCAGCTACTGGATGACACCATTGGAATCCACCCCACCTGTGGTGAG
  GTATTTACAACCATGGAAATCACAAAGTCCTCAGGGCTGGACATTACTCAGAAAGGCTGCTGAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217048 representing NM_001184712
  Red=Cloning site Green=Tags(s)
  
  LEKPPSPPPPPRAQTSPGQGKAGVVPNRRLGAVRGGLMSSPPGRRARLTSPGTSRPPAEVREEVRRRLRD
  LIEGNRVMIFSKSYCPHSSRVKELFSSLGVNYYILELDQVDDGANVQEVLTEISNQKTVPNIFVNKVHVG
  GCDRIFQAHQNGLLQKLLQDDSTHDYDLIVIGGGSGGLSCAKEAANLGKKVMVLDFVVPSPQGTSWGLGG
  TCVNVGCIPKKLMHQAALLGHALQDARKYGWDYNQQVKHNWETMREAIQNHIGSLNWGYRVTLREKGVTY
  VNSFGEFVELHKIKATNKKGQETFYTASKFVIATGERPRYLGIQGDKEYCITSDDLFSLPYCPGNTLIVG
  ASYVGLECAGFLAGLGLDVTVMVRSVLLRGFDQEMAEKVGSYLEQQGVKFQRKFTPILVQQLEKGLPGRL
  KVVAKSTEGPETVEGTYNTVLLAIGRDSCTRKIGLEKIGVKINEKNGKIPVNDVEQTNVPHVYAIGDVLE
  GKPELTPVAIQAGKLLARRLFGISLEKCDYINVPTTVFTPLEYGCCGLSEEKAIEMYTKENLEVYHTFFW
  PLEWTVAGRDNNTCYAKIICNKFDNDRVIGFHLLGPNAGEVTQGFAAAMKCGLTKQLLDDTIGIHPTCGE
  VFTTMEITKSSGLDITQKGC*G
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184712
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184712.1, NP_001171641.1
• RefSeq Size: 2799 bp
• RefSeq ORF: 1959 bp
• Locus ID: 297437
• Cytogenetics: 4q34
• MW: 71.6 kDa
• Gene Summary: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes the third TrxR, which unlike the other two isozymes, contains an additional N-terminal glutaredoxin (Grx) domain, and shows highest expression in testis. The Grx domain allows this isozyme to participate in both Trx and glutathione systems. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. There is evidence for two isoforms resulting from the use of a non-AUG (CUG), and an in-frame downstream AUG as translation initiation codons (PMID:20018845). [provided by RefSeq, Aug 2017]