Txnrd3 (NM_001184712) Rat Untagged Clone
CAT#: RN217048
Txnrd3 (untagged) - Rat thioredoxin reductase 3 (Txnrd3), transcript variant 1
"NM_001184712" in other vectors (1)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Symbol | Txnrd3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN217048 representing NM_001184712
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGGAGAAGCCACCGTCCCCGCCGCCGCCTCCTCGGGCGCAAACCTCGCCTGGGCAGGGGAAGGCTGGAG TCGTCCCCAACCGCCGCCTGGGTGCTGTTCGTGGCGGCCTCATGTCGTCGCCACCCGGCCGCCGCGCCCG CCTGACGTCCCCCGGGACCAGCCGCCCGCCCGCTGAGGTCCGCGAGGAGGTGCGGCGCCGCCTGCGGGAT CTCATCGAGGGCAACAGGGTTATGATCTTCAGCAAGAGTTACTGTCCACACAGCTCGCGGGTTAAGGAAC TCTTTTCATCCTTGGGAGTGAACTATTACATCCTGGAACTTGATCAAGTTGATGATGGGGCCAATGTTCA GGAAGTGCTGACAGAAATCAGTAACCAGAAAACGGTGCCCAATATTTTTGTGAATAAAGTGCACGTGGGT GGATGTGACCGGATTTTCCAGGCACATCAGAATGGTTTACTGCAGAAGCTCCTTCAGGATGACTCAACTC ATGATTATGATCTCATCGTCATCGGCGGGGGCTCTGGCGGCCTCTCTTGTGCCAAGGAAGCTGCCAACTT GGGAAAGAAGGTCATGGTGCTAGACTTTGTGGTTCCCTCGCCTCAGGGCACGTCCTGGGGCCTTGGCGGC ACCTGTGTGAATGTAGGCTGTATCCCTAAGAAGCTGATGCATCAGGCAGCCCTCCTGGGGCATGCTCTGC AAGATGCGAGGAAATATGGCTGGGATTATAACCAGCAGGTGAAGCACAACTGGGAGACCATGAGGGAAGC GATCCAGAACCACATTGGCTCCTTGAACTGGGGCTACAGGGTAACTCTTCGGGAGAAAGGCGTGACCTAT GTCAACTCCTTCGGGGAGTTTGTGGAACTGCATAAAATAAAGGCAACCAATAAGAAAGGGCAGGAAACTT TTTACACCGCTTCGAAGTTTGTCATAGCAACAGGGGAAAGGCCGCGGTACTTGGGAATCCAGGGAGATAA AGAGTACTGTATTACCAGCGACGACCTGTTCTCTCTGCCATACTGCCCAGGGAACACATTAATCGTTGGC GCCTCGTATGTTGGTCTGGAGTGTGCAGGCTTTTTGGCTGGCTTGGGGCTAGATGTGACAGTCATGGTAC GCTCTGTCCTTCTTCGTGGCTTTGATCAAGAAATGGCAGAGAAAGTGGGATCCTACCTGGAACAACAAGG CGTCAAGTTCCAAAGGAAATTCACCCCCATTTTGGTTCAACAGTTGGAGAAAGGCTTACCCGGAAGATTG AAAGTCGTGGCTAAGTCCACTGAAGGACCAGAGACAGTAGAAGGGACATACAACACGGTTTTGTTAGCAA TTGGTCGTGACTCCTGTACAAGGAAAATAGGGCTGGAGAAGATCGGGGTCAAAATCAATGAGAAGAATGG CAAAATACCAGTAAATGATGTGGAACAGACCAATGTGCCTCATGTTTATGCTATTGGGGACGTACTGGAG GGCAAACCAGAGCTCACACCTGTTGCCATACAGGCAGGCAAGCTGCTAGCTCGAAGACTCTTTGGGATCT CTTTAGAAAAGTGTGATTACATTAACGTCCCAACAACGGTGTTCACACCTCTGGAATACGGCTGTTGTGG ACTGTCTGAAGAGAAAGCCATTGAAATGTATACGAAGGAGAATCTGGAAGTGTATCACACCTTCTTTTGG CCTCTTGAGTGGACAGTTGCTGGCAGAGACAACAACACCTGTTATGCAAAGATAATCTGCAACAAATTTG ACAATGACCGTGTGATAGGATTTCACCTCCTGGGGCCAAATGCTGGTGAAGTCACACAGGGGTTTGCAGC TGCAATGAAATGTGGGCTTACGAAGCAGCTACTGGATGACACCATTGGAATCCACCCCACCTGTGGTGAG GTATTTACAACCATGGAAATCACAAAGTCCTCAGGGCTGGACATTACTCAGAAAGGCTGCTGAGGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001184712 |
Insert Size | 1959 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001184712.1, NP_001171641.1 |
RefSeq Size | 2799 bp |
RefSeq ORF | 1959 bp |
Locus ID | 297437 |
Cytogenetics | 4q34 |
Gene Summary | The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes the third TrxR, which unlike the other two isozymes, contains an additional N-terminal glutaredoxin (Grx) domain, and shows highest expression in testis. The Grx domain allows this isozyme to participate in both Trx and glutathione systems. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. There is evidence for two isoforms resulting from the use of a non-AUG (CUG), and an in-frame downstream AUG as translation initiation codons (PMID:20018845). [provided by RefSeq, Aug 2017] Transcript Variant: This variant (1) contains two in-frame translation initiation codons: an upstream non-AUG (CUG) and a downstream AUG codon. The longer isoform (1) represented in this RefSeq results from the use of the CUG start codon. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript and the encoded protein are supported by orthologous mouse data. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR217048 | Txnrd3 (myc-DDK-tagged) - Rat thioredoxin reductase 3 (Txnrd3), transcript variant 1 |
USD 667.00 |
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