Hrh3 (NM_001270570) Rat Tagged ORF Clone

CAT#: RR216431

Hrh3 (myc-DDK-tagged) - Rat histamine receptor H3 (Hrh3), transcript variant 8

Product Images

Circular map for RR216431

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hrh3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216431 representing NM_001270570
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGCGCGCCGCCCGACGGGCTGATGAACGCGTCGGGCACTCTGGCCGGAGAGGCGGCGGCTGCAG
  GCGGGGCGCGCGGCTTCTCGGCTGCCTGGACCGCTGTCCTGGCTGCGCTCATGGCGCTGCTCATCGTGGC
  CACAGTACTGGGCAACGCGCTGGTCATGCTCGCCTTCGTGGCGGATTCGAGCCTCCGCACCCAGAACAAC
  TTCTTTCTGCTCAACCTCGCCATCTCCGACTTCCTCGTGGGTGCCTTCTGCATCCCATTGTACGTACCCT
  ATGTGCTGACCGGCCGTTGGACCTTCGGCCGGGGCCTCTGCAAGCTGTGGCTGGTGGTAGACTACCTACT
  GTGTGCCTCCTCGGTCTTCAACATCGTACTCATCAGCTATGACCGATTCCTGTCAGTCACTCGAGCTGTC
  TCCTACAGGGCCCAGCAGGGGGACACGAGACGGGCCGTTCGGAAGATGGCACTGGTGTGGGTGCTGGCCT
  TCCTGCTGTATGGGCCTGCCATCCTGAGTTGGGAGTACCTGTCTGGTGGCAGTTCCATCCCCGAGGGCCA
  CTGCTATGCTGAGTTCTTCTACAACTGGTACTTTCTCATCACGGCCTCCACCCTCGAGTTCTTCACGCCC
  TTCCTCAGCGTTACCTTCTTCAACCTCAGCATCTACCTGAACATCCAGAGGCGCACCCGCCTTCGGCTTG
  ATGGGGGCCGTGAGGCTGGCCCAGAACCCCCACCAGATGCCCAGCCCTCGCCACCTCCAGCTCCCCCCAG
  CTGCTGGGGCTGCTGGCCAAAAGGGCATGGCGAGGCCATGCCGTTGCACAGGGGCTCCAAGCCATCAGCA
  TCTTCAGCATCCCTGGAGAAGCGCATGAAGATGGTGTCCCAGAGCATCACCCAGCGCTTCCGGCTGTCGC
  GGGACAAGAAGGTGGCCAAGTCGCTGGCCATCATCGTGAGCATCTTTGGGCTCTGCTGGGCGCCGTACAC
  GCTCCTAATGATCATCCGAGCTGCTTGCCATGGCCGCTGCATCCCCGATTAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216431 representing NM_001270570
  Red=Cloning site Green=Tags(s)
  
  MERAPPDGLMNASGTLAGEAAAAGGARGFSAAWTAVLAALMALLIVATVLGNALVMLAFVADSSLRTQNN
  FFLLNLAISDFLVGAFCIPLYVPYVLTGRWTFGRGLCKLWLVVDYLLCASSVFNIVLISYDRFLSVTRAV
  SYRAQQGDTRRAVRKMALVWVLAFLLYGPAILSWEYLSGGSSIPEGHCYAEFFYNWYFLITASTLEFFTP
  FLSVTFFNLSIYLNIQRRTRLRLDGGREAGPEPPPDAQPSPPPAPPSCWGCWPKGHGEAMPLHRGSKPSA
  SSASLEKRMKMVSQSITQRFRLSRDKKVAKSLAIIVSIFGLCWAPYTLLMIIRAACHGRCIPDY
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270570
• ORF Size: 1032 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270570.1, NP_001257499.1
• RefSeq Size: 1956 bp
• RefSeq ORF: 1035 bp
• Locus ID: 85268
• Cytogenetics: 3q43
• MW: 37.8 kDa
• Gene Summary: This gene encodes a histamine H3 receptor that belongs to the superfamily of G-protein coupled receptors. This protein functions as a presynaptic autoreceptor on histamine neurons in the brain, and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. It is deemed a great target for the development of therapeutics for numerous disorders, including obesity, epilepsy, and such cognitive diseases as attention deficit hyperactivity disorder and Alzheimer's disease. Several alternatively spliced transcript variants encoding different isoforms, with different brain expression patterns and signaling properties, have been described for this gene. [provided by RefSeq, Jul 2012]