Acp2 (NM_016988) Rat Tagged ORF Clone

CAT#: RR213595

Acp2 (Myc-DDK-tagged ORF) - Rat acid phosphatase 2, lysosomal (Acp2), (10 ug)

Product Images

Circular map for RR213595

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acp2
• Synonyms: LAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213595 representing NM_016988
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCAGACAGTCTGGTTGGAGCCAGGCGGCTCTTCTCCAGTTCCTTCTTGGCATGTGCCTAATGG
  TGATGCCACCCATACAAGCCCGGAGTCTGCGCTTTGTTACCTTGCTGTATCGACACGGAGATCGGTCACC
  AGTGAAGGCATATCCTAAGGACCCCTATCAGGAAGAGAAATGGCCCCAGGGATTTGGTCAGCTAACCAAG
  GAAGGGATGCTACAGCATTGGGAGCTGGGCCAGGCCCTGCGGCAACGCTACCATGGCTTTCTGAACGCCT
  CTTACCACAGGCAAGAGGTTTACGTGCGAAGCACAGACTTTGACCGTACTCTCATGAGTGCAGAGGCCAA
  CCTGGCCGGACTCTTCCCTCCCACTGAAGTTCAGCACTTCAACCCGAACATTTCATGGCAGCCTATCCCT
  GTCCACACCGTGCCCATTACTGAAGACAGGTTGCTGAAGTTTCCTTTGGGTCCATGTCCCCGTTATGAGC
  AGTTGCAGAACGAGACTCGGCAGACACCAGAGTATCAGAACATGAGTATTCAGAATGCACAATTTCTGGA
  CATGGTGGCCAATGAGACAGGGCTTATGAACTTGACCCTAGAGACCATCTGGAATGTGTATGACACACTC
  TTTTGTGAGCAAACACATGGGCTGCTCCTGCCACCCTGGGCCTCTCCCCAAACCGTGCAGCGTCTGAGCC
  AGCTAAAGGACTTCAGCTTCCTCTTCCTCTTCGGGATCCACGATCAAGTACAGAAGGCCCGGCTTCAGGG
  GGGAGTTCTGCTGGCTCAAATATTGAAGAATCTGACCCTAATGGCAACTACCTCTCAATTCCCTAAGCTT
  CTGGTTTATTCTGCGCATGACACTACCCTGGTTGCTCTGCAAATGGCACTGAATGTCTACAATGGTAAAC
  AAGCCCCCTATGCTTCCTGCCACATATTTGAACTGTACCAGGAAGATAATGGGAATTTCTCAGTCGAGAT
  GTACTTTCGGAATGACAGTAAGAAGGCACCCTGGCCACTGACCCTGCCTGGCTGTCCTCACCGTTGCCCA
  CTGCAGGACTTCCTTCGCCTCACAGAACCTGTCATACCCAAGGACTGGCAGAAGGAGTGCCAGCTAGCAA
  GCGATACTGCAGACACAGAGGTGATTGTGGCACTGGCTGTCTGTGGCTCCATCCTCTTCCTTCTAATAGT
  GTTGCTCCTCACTGTCCTCTTCCGGATGCAGGCCCAGCCTCCTGGCTACCACCATGTTGCAGACAGGGAA
  GACCATGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213595 representing NM_016988
  Red=Cloning site Green=Tags(s)
  
  MAGRQSGWSQAALLQFLLGMCLMVMPPIQARSLRFVTLLYRHGDRSPVKAYPKDPYQEEKWPQGFGQLTK
  EGMLQHWELGQALRQRYHGFLNASYHRQEVYVRSTDFDRTLMSAEANLAGLFPPTEVQHFNPNISWQPIP
  VHTVPITEDRLLKFPLGPCPRYEQLQNETRQTPEYQNMSIQNAQFLDMVANETGLMNLTLETIWNVYDTL
  FCEQTHGLLLPPWASPQTVQRLSQLKDFSFLFLFGIHDQVQKARLQGGVLLAQILKNLTLMATTSQFPKL
  LVYSAHDTTLVALQMALNVYNGKQAPYASCHIFELYQEDNGNFSVEMYFRNDSKKAPWPLTLPGCPHRCP
  LQDFLRLTEPVIPKDWQKECQLASDTADTEVIVALAVCGSILFLLIVLLLTVLFRMQAQPPGYHHVADRE
  DHA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016988
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016988.2, NP_058684.2
• RefSeq Size: 2067 bp
• RefSeq ORF: 1272 bp
• Locus ID: 24162
• Cytogenetics: 3q24
• MW: 48.4 kDa
• Gene Summary: The protein encoded by this gene belongs to the histidine acid phosphatase family, which hydrolyze orthophosphoric monoesters to alcohol and phosphate. This protein is localized to the lysosomal membrane, and is chemically and genetically distinct from the red cell acid phosphatase. Mice lacking this gene showed multiple defects, including bone structure alterations, lysosomal storage defects, and an increased tendency towards seizures. An enzymatically-inactive allele of this gene showed severe growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2017]