Ccdc93 (NM_001024997) Rat Untagged Clone

CAT#: RN213082

Ccdc93 (untagged ORF) - Rat coiled-coil domain containing 93 (Ccdc93), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ccdc93
• Synonyms: MGC109336
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN213082 representing NM_001024997
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTTACCCAAGGGGCCGGAGGGCCAGGGGCTCCCGGAGGTGGAAACGAGAGAAGACGAAGAACAGA
  ATGTCAAGCTGACTGAAATCCTGGAGCTCTTGGTTGCGGCTGGCTATTTCAGAGCAAGAATTAAAGGCTT
  GTCTCCTTTCGACAAGGTAGTAGGAGGAATGACGTGGTGTATCACCACTTGCAGCTTTGATGTGGATGTG
  GATTTGCTCTTTCAGGAAAACTCTACAATCGGTCAGAAAATAGCTCTGTCGGAAAAGATCGTCTCGGTCC
  TCCCAAGGATGAAGTGCCCCCACCAGCTGGAGCCCCACCAGATCCAGGGCATGGATTTTATCCACATCTT
  CCCTGTCGTGCAGTGGCTGGTGAAGCGAGCTATAGAGACAAAGGAAGAGATGGGCGACTACATCCGCTCC
  TACTCCATCTCCCAGTTCCAGAAGACCTACAGCCTCCCGGAGGATGATGACTTCATAAAGAGAAAAGACA
  AAGCCATCCAGACAGTCGTTGACCTCTCGGATGCTTACAAGCCCCGGCGAAAGTACAGACGGCAGCAGGG
  AGCAGAGGAGCTACTGGACGAGGAGTCCCGGGTCCACTCCACGCTTCTGGAATATGGCAGGAGACACGGC
  TTTAGTCGTCAGAGCAAGACAGAGAAGGCTGAGGACAAGAAAACAGCGCTTGCTGCGGGGCTCTCAGCTG
  CAGAGAAAACGGATGCTCACGAGGAAGATGAGCTTCAAGCAGCTGAGGAGCAACGAATTCAGTCACTGAT
  GACCAAGATGACCGCCATGGCAAATGAGGAGAGCCGTCTCACCGCAAGCTCCGTGGGCCAGATCGTGGGT
  CTCTGCTCCGCCGAGATCAAACAGATTGTGTCTGAGTACGAAGGGAAGCAGTCTGAGCTGTCCGCTGAAG
  AAAGTCCAGAGAAATTAGGAACCTCCCAACTGCATCAGCGGAAGGTCATTTCCTTGAATAAACAGATTCT
  GCAGAAGACCAAACATCTAGAAGAGCTGCAAGCGAATCATACCAGCCTCCAAGCCAAGTACAGCGACAAG
  AAGAAGACGCTGACAGAGCTGAAGAATCACGGTGAGAAACTAGACCAAGAGCAGGCAGCCCTGGAGAAGT
  TAGAAGCCAAAGCCGACCCGAGCATTCTGCAGAACCTGAGAGCACTCGTCGCCATGAATGAAAGTCTGAA
  AAGCCAGGAACAGGAGTTTAAAGCGCACTGCCGGGAGGAGATGGCTCGGCTCCAACAGGAAATTGAGATC
  CTGAAAGCCGAGAGAGCGCCAGGCGAGAAGACTATCTCCAGCGGAGAGCCGCAGGGTGCCCTGACCTCCA
  CCATGACTCACAATGAGGACCTGGACAGACGGTACAACATGGAAAAAGAGAAGCTGTACAAGATCCGGTT
  ACTACAGGCAAGAAGAAATCGAGAAATAGCGATTTTGCACCGCAAGATTGATGAAGTGCCCAGTCGAGCA
  GAGCTGATACAGTATCAGAAGCGATTTATTGAGCTCTACCGTCAGATTTCCGCGGTGCACAAGGAGACCA
  AGCAGTTCTTCACTTTGTACAATACCCTGGATGACAAAAAGGTTTATCTAGAAAAGGAGATTAGCCTGCT
  AAACTCAATTCATGAGAACTTCTCACAAGCCATGGCCTCCCCTGCTGCCCGGGACCAGTTTTTAAGGCAG
  ATGGAACAGATCGTCGAAGGCATTAAACAAAGCAGGATGAAGATGGAAAAGAAGAAGCAGGAGAACAAGA
  TGAGAAGGGACCAGTTGAACGATCAGTACTTGGAGCTCCTGGAAAAGCAGCGGCTCTACTTCAAGACTGT
  AAAAGAGTTCAAAGAGGAGGGCCGAAAGAATGAGCTATTACTGTCTAAGATCAAAGCCAAGGCCTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001024997
• Insert Size: 1890 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024997.1, NP_001020168.1
• RefSeq Size: 2403 bp
• RefSeq ORF: 1890 bp
• Locus ID: 304743
• UniProt ID: Q5BJT7
• Cytogenetics: 13q11
• Gene Summary: Component of the CCC complex, which is involved in the regulation of endosomal recycling of surface proteins, including integrins, signaling receptor and channels. The CCC complex associates with SNX17, retriever and WASH complexes to prevent lysosomal degradation and promote cell surface recycling of numerous cargos such as integrins ITGA5:ITGB1. Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes and is dependent on its interaction with WASHC2C.[UniProtKB/Swiss-Prot Function]