Ccdc93 (NM_001024997) Rat Tagged ORF Clone

CAT#: RR213082

Ccdc93 (Myc-DDK-tagged ORF) - Rat coiled-coil domain containing 93 (Ccdc93), (10 ug)

Product Images

Circular map for RR213082

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccdc93
• Synonyms: MGC109336
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213082 representing NM_001024997
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTTACCCAAGGGGCCGGAGGGCCAGGGGCTCCCGGAGGTGGAAACGAGAGAAGACGAAGAACAGA
  ATGTCAAGCTGACTGAAATCCTGGAGCTCTTGGTTGCGGCTGGCTATTTCAGAGCAAGAATTAAAGGCTT
  GTCTCCTTTCGACAAGGTAGTAGGAGGAATGACGTGGTGTATCACCACTTGCAGCTTTGATGTGGATGTG
  GATTTGCTCTTTCAGGAAAACTCTACAATCGGTCAGAAAATAGCTCTGTCGGAAAAGATCGTCTCGGTCC
  TCCCAAGGATGAAGTGCCCCCACCAGCTGGAGCCCCACCAGATCCAGGGCATGGATTTTATCCACATCTT
  CCCTGTCGTGCAGTGGCTGGTGAAGCGAGCTATAGAGACAAAGGAAGAGATGGGCGACTACATCCGCTCC
  TACTCCATCTCCCAGTTCCAGAAGACCTACAGCCTCCCGGAGGATGATGACTTCATAAAGAGAAAAGACA
  AAGCCATCCAGACAGTCGTTGACCTCTCGGATGCTTACAAGCCCCGGCGAAAGTACAGACGGCAGCAGGG
  AGCAGAGGAGCTACTGGACGAGGAGTCCCGGGTCCACTCCACGCTTCTGGAATATGGCAGGAGACACGGC
  TTTAGTCGTCAGAGCAAGACAGAGAAGGCTGAGGACAAGAAAACAGCGCTTGCTGCGGGGCTCTCAGCTG
  CAGAGAAAACGGATGCTCACGAGGAAGATGAGCTTCAAGCAGCTGAGGAGCAACGAATTCAGTCACTGAT
  GACCAAGATGACCGCCATGGCAAATGAGGAGAGCCGTCTCACCGCAAGCTCCGTGGGCCAGATCGTGGGT
  CTCTGCTCCGCCGAGATCAAACAGATTGTGTCTGAGTACGAAGGGAAGCAGTCTGAGCTGTCCGCTGAAG
  AAAGTCCAGAGAAATTAGGAACCTCCCAACTGCATCAGCGGAAGGTCATTTCCTTGAATAAACAGATTCT
  GCAGAAGACCAAACATCTAGAAGAGCTGCAAGCGAATCATACCAGCCTCCAAGCCAAGTACAGCGACAAG
  AAGAAGACGCTGACAGAGCTGAAGAATCACGGTGAGAAACTAGACCAAGAGCAGGCAGCCCTGGAGAAGT
  TAGAAGCCAAAGCCGACCCGAGCATTCTGCAGAACCTGAGAGCACTCGTCGCCATGAATGAAAGTCTGAA
  AAGCCAGGAACAGGAGTTTAAAGCGCACTGCCGGGAGGAGATGGCTCGGCTCCAACAGGAAATTGAGATC
  CTGAAAGCCGAGAGAGCGCCAGGCGAGAAGACTATCTCCAGCGGAGAGCCGCAGGGTGCCCTGACCTCCA
  CCATGACTCACAATGAGGACCTGGACAGACGGTACAACATGGAAAAAGAGAAGCTGTACAAGATCCGGTT
  ACTACAGGCAAGAAGAAATCGAGAAATAGCGATTTTGCACCGCAAGATTGATGAAGTGCCCAGTCGAGCA
  GAGCTGATACAGTATCAGAAGCGATTTATTGAGCTCTACCGTCAGATTTCCGCGGTGCACAAGGAGACCA
  AGCAGTTCTTCACTTTGTACAATACCCTGGATGACAAAAAGGTTTATCTAGAAAAGGAGATTAGCCTGCT
  AAACTCAATTCATGAGAACTTCTCACAAGCCATGGCCTCCCCTGCTGCCCGGGACCAGTTTTTAAGGCAG
  ATGGAACAGATCGTCGAAGGCATTAAACAAAGCAGGATGAAGATGGAAAAGAAGAAGCAGGAGAACAAGA
  TGAGAAGGGACCAGTTGAACGATCAGTACTTGGAGCTCCTGGAAAAGCAGCGGCTCTACTTCAAGACTGT
  AAAAGAGTTCAAAGAGGAGGGCCGAAAGAATGAGCTATTACTGTCTAAGATCAAAGCCAAGGCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213082 representing NM_001024997
  Red=Cloning site Green=Tags(s)
  
  MGLPKGPEGQGLPEVETREDEEQNVKLTEILELLVAAGYFRARIKGLSPFDKVVGGMTWCITTCSFDVDV
  DLLFQENSTIGQKIALSEKIVSVLPRMKCPHQLEPHQIQGMDFIHIFPVVQWLVKRAIETKEEMGDYIRS
  YSISQFQKTYSLPEDDDFIKRKDKAIQTVVDLSDAYKPRRKYRRQQGAEELLDEESRVHSTLLEYGRRHG
  FSRQSKTEKAEDKKTALAAGLSAAEKTDAHEEDELQAAEEQRIQSLMTKMTAMANEESRLTASSVGQIVG
  LCSAEIKQIVSEYEGKQSELSAEESPEKLGTSQLHQRKVISLNKQILQKTKHLEELQANHTSLQAKYSDK
  KKTLTELKNHGEKLDQEQAALEKLEAKADPSILQNLRALVAMNESLKSQEQEFKAHCREEMARLQQEIEI
  LKAERAPGEKTISSGEPQGALTSTMTHNEDLDRRYNMEKEKLYKIRLLQARRNREIAILHRKIDEVPSRA
  ELIQYQKRFIELYRQISAVHKETKQFFTLYNTLDDKKVYLEKEISLLNSIHENFSQAMASPAARDQFLRQ
  MEQIVEGIKQSRMKMEKKKQENKMRRDQLNDQYLELLEKQRLYFKTVKEFKEEGRKNELLLSKIKAKAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024997
• ORF Size: 1887 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024997.1, NP_001020168.1
• RefSeq Size: 2403 bp
• RefSeq ORF: 1890 bp
• Locus ID: 304743
• UniProt ID: Q5BJT7
• Cytogenetics: 13q11
• MW: 72.6 kDa
• Gene Summary: Component of the CCC complex, which is involved in the regulation of endosomal recycling of surface proteins, including integrins, signaling receptor and channels. The CCC complex associates with SNX17, retriever and WASH complexes to prevent lysosomal degradation and promote cell surface recycling of numerous cargos such as integrins ITGA5:ITGB1. Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes and is dependent on its interaction with WASHC2C.[UniProtKB/Swiss-Prot Function]