Pde11a (NM_080893) Rat Untagged Clone

CAT#: RN200214

Pde11a (untagged ORF) - Rat phosphodiesterase 11A (Pde11a), transcript variant 4, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pde11a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200214 representing NM_080893
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCTCCCGCCTGGACTTCGGGGAGGTGGAAACTTTCCTGGATAGGCACCCAGAATTGTTTGAAG
  ATTACTTGATGCGAAAAGGAAAGCAGGAGCTGGTGGACAAGTGGCTACAAAGGCACAGTTCGGGACAGGG
  GGCTTCAGACCTGAGGCCTGCCCTGGCTGGCGCTAGCAGCTTGGCTCAGAGCAGTGCCAGAGGCAGCACT
  GGCATAGGTGGTGGTGCTGGACCCCAGGGTTCTGCCAACAGTCATCCCGCGTCCGGTGGTGGGGAAAGTG
  CCGGGGTTCCTCTGAGTCCCAGTTGGGCCAGTGGCAGCCGGGGCGATGGCAACCTTCAGAGGAGAGCTTC
  GCAGAAAGAGCTGAGAAAGAGCTTTGCCCGCTCCAAAGCCATCCATGTGAACAGGACCTACGACGAACAG
  GTGACCTCCCGGGCCCAGGAGCCCCTGAGCAGTGTCCGGAGGCGAGCCCTGCTCAGAAAGGCCAGCTCTC
  TGCCCCCCACCACAGCCCACATTCTCAGTGCCCTGCTGGAATCGAGGGTGAATCTTCCTCAGTATCCCCC
  TACGGCCATCGACTACAAATGCCACCTCAAAAAGCATAATGAGCGACAGTTTTTCCTGGAACTGGTCAAG
  GACATCTCCAATGACCTTGACCTCACCAGCCTAAGCTACAAGATCCTCATCTTTGTCTGTCTCATGGTGG
  ACGCTGACCGCTGCTCTCTCTTCTTAGTGGAAGGGGCAGCTGCTGGTAAGAAGACTTTGGTCTCCAAGTT
  TTTTGACGTGCATGCAGGAACCCCACTGCTGCCCTGCAGCACCACAGAGAACTCAAATGAGGTGCAGGTC
  CCCTGGGGCAAAGGTATCATTGGCTATGTCGGGGAACACGGAGAAACAGTCAACATTCCCGATGCCTACC
  AGGACCGAAGATTCAATGATGAAATTGACAAGCTGACTGGATACAAGACAAAATCACTATTGTGCATGCC
  TATCCGGAACAGTGACGGTGAGATTATCGGTGTGGCCCAGGCGATAAATAAGGTTCCTGAGGGTGCTCCA
  TTTACAGAAGACGACGAAAAAGTTATGCAGATGTATCTTCCATTCTGTGGCATCGCCATATCTAATGCTC
  AGCTCTTCGCCGCCTCGAGGAAAGAATATGAAAGAAGTAGGGCCTTGCTGGAGGTGGTCAATGACCTCTT
  TGAAGAACAGACTGACCTGGAAAAGATTGTCAAGAAAATAATGCATCGGGCCCAAACTCTGTTGAAATGT
  GAACGCTGTTCCGTTTTACTTCTAGAAGACATTGAATCACCAGTGGTGAAGTTTACCAAATCCTTTGAAC
  TGATGTCCCCAAAGTGCAGTGCGGACGCGGAGAACAGTTTCAAAGAAAGTGTGGAGAAGTCATCTTACTC
  CGACTGGCTGATAAATAACAGTATCGCTGAGCTGGTTGCTTCGACAGGCCTTCCTGTGAATGTCAGCGAT
  GCCTACCAGGACCCACGCTTTGACGCTGAGGCTGACCAGATATCTGGCTTTCATATAAGATCTGTTCTCT
  GTGTCCCTATTTGGAACAGCAACCACCAAATAATAGGGGTCGCTCAAGTGCTGAACAGACTCGATGGGAA
  ACCTTTTGATGATGCTGACCAAAGGCTTTTTGAGGCCTTTGTCATCTTTTGTGGCCTTGGTATTAACAAC
  ACGATTATGTATGACCAAGTGAAGAAGTCCTGGGCCAAGCAGTCCGTGGCTCTTGATGTGCTGTCCTACC
  ACGCCACGTGTTCCAAGGCTGAAGTTGACAAGTTTAAGGCAGCCAACATCCCCCTGGTGTCGGAACTGGC
  CATCGATGACATCCATTTTGATGACTTTTCCCTTGATGTTGATGCCATGATCACAGCCGCTCTACGGATG
  TTCATGGAGCTGGGGATGGTACAGAAATTTAAAATCGACTATGAGACCCTGTGCAGGTGGCTTCTGACAG
  TAAGGAAAAACTATCGGATGGTTCTCTACCACAACTGGAGACATGCCTTCAACGTGTGCCAGCTGATGTT
  TGCCATGCTAACTACTGCTGGGTTTCAAGAGATTCTGACCGAGGTGGAAATTTTAGCGGTGATTGTGGGA
  TGCCTGTGTCATGACCTCGACCACAGGGGAACCAACAATGCCTTCCAAGCTAAGAGTGACTCTGCACTGG
  CCCAGCTCTATGGGACCTCAGCGACCTTAGAGCATCACCACTTTAACCACGCCGTGATGATCCTTCAGAG
  TGAGGGTCACAACATCTTTGCTAATTTGTCCTCCAAGGAATACAGCGACCTCATGCAGCTCCTGAAGCAG
  TCGATACTAGCCACTGACCTCACGCTGTACTTCGAGAGAAGAACTGAGTTCTTCGAGCTTGTCAGTAAAG
  GAGCCTATGATTGGAGCATCACAAGTCACCGCGATGTGTTTCGATCAATGTTAATGACAGCTTGTGACCT
  TGGAGCCGTGACCAAACCGTGGGAGATCTCCAGACAGGTGGCTGAACTTGTCACCAGCGAGTTCTTCGAA
  CAAGGAGATCGGGAGAGGTCGGAACTCAAGCTCACCCCCTCTGCTATTTTTGACCGGAACCGGAAAGATG
  AGCTGCCTCGGCTGCAACTGGAGTGGATTGACAGCATCTGCATGCCTTTGTATCAGGCCTTGGTGAAAGT
  CAATGCAAAACTGAAGCCGATGCTGGACTCAGTGGCCGCCAACCGCAGGAAGTGGGAAGAGTTGCACCAA
  AAAAGACTACAGGTCTCTGCTGCCTCCCCAGTCCCTTCCAGTCCCAGCCCAGCGGTGGCCGGAGAGGACA
  GACTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_080893
• Insert Size: 2808 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080893.1, NP_543169.1
• RefSeq Size: 4170 bp
• RefSeq ORF: 2808 bp
• Locus ID: 140928
• UniProt ID: Q8VID6
• Cytogenetics: 3q23-q24
• Gene Summary: hydrolyzes cyclic nucleotides cAMP and cGMP, downregulating their activity [RGD, Feb 2006]
  Transcript Variant: This variant (4), also known as PDE11A4, represents the longest transcript and encodes the longest protein (isoform 4).