Pde11a (NM_080893) Rat Tagged ORF Clone

CAT#: RR200214

Pde11a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 11A (Pde11a), transcript variant 4, (10 ug)

Product Images

Circular map for RR200214

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde11a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200214 representing NM_080893
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCTCCCGCCTGGACTTCGGGGAGGTGGAAACTTTCCTGGATAGGCACCCAGAATTGTTTGAAG
  ATTACTTGATGCGAAAAGGAAAGCAGGAGCTGGTGGACAAGTGGCTACAAAGGCACAGTTCGGGACAGGG
  GGCTTCAGACCTGAGGCCTGCCCTGGCTGGCGCTAGCAGCTTGGCTCAGAGCAGTGCCAGAGGCAGCACT
  GGCATAGGTGGTGGTGCTGGACCCCAGGGTTCTGCCAACAGTCATCCCGCGTCCGGTGGTGGGGAAAGTG
  CCGGGGTTCCTCTGAGTCCCAGTTGGGCCAGTGGCAGCCGGGGCGATGGCAACCTTCAGAGGAGAGCTTC
  GCAGAAAGAGCTGAGAAAGAGCTTTGCCCGCTCCAAAGCCATCCATGTGAACAGGACCTACGACGAACAG
  GTGACCTCCCGGGCCCAGGAGCCCCTGAGCAGTGTCCGGAGGCGAGCCCTGCTCAGAAAGGCCAGCTCTC
  TGCCCCCCACCACAGCCCACATTCTCAGTGCCCTGCTGGAATCGAGGGTGAATCTTCCTCAGTATCCCCC
  TACGGCCATCGACTACAAATGCCACCTCAAAAAGCATAATGAGCGACAGTTTTTCCTGGAACTGGTCAAG
  GACATCTCCAATGACCTTGACCTCACCAGCCTAAGCTACAAGATCCTCATCTTTGTCTGTCTCATGGTGG
  ACGCTGACCGCTGCTCTCTCTTCTTAGTGGAAGGGGCAGCTGCTGGTAAGAAGACTTTGGTCTCCAAGTT
  TTTTGACGTGCATGCAGGAACCCCACTGCTGCCCTGCAGCACCACAGAGAACTCAAATGAGGTGCAGGTC
  CCCTGGGGCAAAGGTATCATTGGCTATGTCGGGGAACACGGAGAAACAGTCAACATTCCCGATGCCTACC
  AGGACCGAAGATTCAATGATGAAATTGACAAGCTGACTGGATACAAGACAAAATCACTATTGTGCATGCC
  TATCCGGAACAGTGACGGTGAGATTATCGGTGTGGCCCAGGCGATAAATAAGGTTCCTGAGGGTGCTCCA
  TTTACAGAAGACGACGAAAAAGTTATGCAGATGTATCTTCCATTCTGTGGCATCGCCATATCTAATGCTC
  AGCTCTTCGCCGCCTCGAGGAAAGAATATGAAAGAAGTAGGGCCTTGCTGGAGGTGGTCAATGACCTCTT
  TGAAGAACAGACTGACCTGGAAAAGATTGTCAAGAAAATAATGCATCGGGCCCAAACTCTGTTGAAATGT
  GAACGCTGTTCCGTTTTACTTCTAGAAGACATTGAATCACCAGTGGTGAAGTTTACCAAATCCTTTGAAC
  TGATGTCCCCAAAGTGCAGTGCGGACGCGGAGAACAGTTTCAAAGAAAGTGTGGAGAAGTCATCTTACTC
  CGACTGGCTGATAAATAACAGTATCGCTGAGCTGGTTGCTTCGACAGGCCTTCCTGTGAATGTCAGCGAT
  GCCTACCAGGACCCACGCTTTGACGCTGAGGCTGACCAGATATCTGGCTTTCATATAAGATCTGTTCTCT
  GTGTCCCTATTTGGAACAGCAACCACCAAATAATAGGGGTCGCTCAAGTGCTGAACAGACTCGATGGGAA
  ACCTTTTGATGATGCTGACCAAAGGCTTTTTGAGGCCTTTGTCATCTTTTGTGGCCTTGGTATTAACAAC
  ACGATTATGTATGACCAAGTGAAGAAGTCCTGGGCCAAGCAGTCCGTGGCTCTTGATGTGCTGTCCTACC
  ACGCCACGTGTTCCAAGGCTGAAGTTGACAAGTTTAAGGCAGCCAACATCCCCCTGGTGTCGGAACTGGC
  CATCGATGACATCCATTTTGATGACTTTTCCCTTGATGTTGATGCCATGATCACAGCCGCTCTACGGATG
  TTCATGGAGCTGGGGATGGTACAGAAATTTAAAATCGACTATGAGACCCTGTGCAGGTGGCTTCTGACAG
  TAAGGAAAAACTATCGGATGGTTCTCTACCACAACTGGAGACATGCCTTCAACGTGTGCCAGCTGATGTT
  TGCCATGCTAACTACTGCTGGGTTTCAAGAGATTCTGACCGAGGTGGAAATTTTAGCGGTGATTGTGGGA
  TGCCTGTGTCATGACCTCGACCACAGGGGAACCAACAATGCCTTCCAAGCTAAGAGTGACTCTGCACTGG
  CCCAGCTCTATGGGACCTCAGCGACCTTAGAGCATCACCACTTTAACCACGCCGTGATGATCCTTCAGAG
  TGAGGGTCACAACATCTTTGCTAATTTGTCCTCCAAGGAATACAGCGACCTCATGCAGCTCCTGAAGCAG
  TCGATACTAGCCACTGACCTCACGCTGTACTTCGAGAGAAGAACTGAGTTCTTCGAGCTTGTCAGTAAAG
  GAGCCTATGATTGGAGCATCACAAGTCACCGCGATGTGTTTCGATCAATGTTAATGACAGCTTGTGACCT
  TGGAGCCGTGACCAAACCGTGGGAGATCTCCAGACAGGTGGCTGAACTTGTCACCAGCGAGTTCTTCGAA
  CAAGGAGATCGGGAGAGGTCGGAACTCAAGCTCACCCCCTCTGCTATTTTTGACCGGAACCGGAAAGATG
  AGCTGCCTCGGCTGCAACTGGAGTGGATTGACAGCATCTGCATGCCTTTGTATCAGGCCTTGGTGAAAGT
  CAATGCAAAACTGAAGCCGATGCTGGACTCAGTGGCCGCCAACCGCAGGAAGTGGGAAGAGTTGCACCAA
  AAAAGACTACAGGTCTCTGCTGCCTCCCCAGTCCCTTCCAGTCCCAGCCCAGCGGTGGCCGGAGAGGACA
  GACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200214 representing NM_080893
  Red=Cloning site Green=Tags(s)
  
  MAASRLDFGEVETFLDRHPELFEDYLMRKGKQELVDKWLQRHSSGQGASDLRPALAGASSLAQSSARGST
  GIGGGAGPQGSANSHPASGGGESAGVPLSPSWASGSRGDGNLQRRASQKELRKSFARSKAIHVNRTYDEQ
  VTSRAQEPLSSVRRRALLRKASSLPPTTAHILSALLESRVNLPQYPPTAIDYKCHLKKHNERQFFLELVK
  DISNDLDLTSLSYKILIFVCLMVDADRCSLFLVEGAAAGKKTLVSKFFDVHAGTPLLPCSTTENSNEVQV
  PWGKGIIGYVGEHGETVNIPDAYQDRRFNDEIDKLTGYKTKSLLCMPIRNSDGEIIGVAQAINKVPEGAP
  FTEDDEKVMQMYLPFCGIAISNAQLFAASRKEYERSRALLEVVNDLFEEQTDLEKIVKKIMHRAQTLLKC
  ERCSVLLLEDIESPVVKFTKSFELMSPKCSADAENSFKESVEKSSYSDWLINNSIAELVASTGLPVNVSD
  AYQDPRFDAEADQISGFHIRSVLCVPIWNSNHQIIGVAQVLNRLDGKPFDDADQRLFEAFVIFCGLGINN
  TIMYDQVKKSWAKQSVALDVLSYHATCSKAEVDKFKAANIPLVSELAIDDIHFDDFSLDVDAMITAALRM
  FMELGMVQKFKIDYETLCRWLLTVRKNYRMVLYHNWRHAFNVCQLMFAMLTTAGFQEILTEVEILAVIVG
  CLCHDLDHRGTNNAFQAKSDSALAQLYGTSATLEHHHFNHAVMILQSEGHNIFANLSSKEYSDLMQLLKQ
  SILATDLTLYFERRTEFFELVSKGAYDWSITSHRDVFRSMLMTACDLGAVTKPWEISRQVAELVTSEFFE
  QGDRERSELKLTPSAIFDRNRKDELPRLQLEWIDSICMPLYQALVKVNAKLKPMLDSVAANRRKWEELHQ
  KRLQVSAASPVPSSPSPAVAGEDRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080893
• ORF Size: 2805 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080893.1, NP_543169.1
• RefSeq Size: 4170 bp
• RefSeq ORF: 2808 bp
• Locus ID: 140928
• UniProt ID: Q8VID6
• Cytogenetics: 3q23-q24
• MW: 104.6 kDa
• Gene Summary: hydrolyzes cyclic nucleotides cAMP and cGMP, downregulating their activity [RGD, Feb 2006]