ZRANB3 (NM_001286569) Human Tagged ORF Clone

CAT#: RG239134

ZRANB3 (tGFP-tagged) - Human zinc finger, RAN-binding domain containing 3 (ZRANB3), transcript variant 3

Product Images

Circular map for RG239134

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZRANB3
• Synonyms: 4933425L19Rik; AH2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239134 representing NM_001286569.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGGGGAATGTTGAATCGCAAGGCTCAAGTTACAGGGAGCACACTGAACGGTAGGAAAGAAAAAATT
  CAGGCTGAGGAAGGTGATAAGGAAAAATGGGATTTCCTGCAGTTTGCTGAAGCTTGGACTCCAAATGAC
  AGTTCTGAAGAGTTAAGGAAGGAAGCTTTGTTCACTCACTTCGAAAAAGAAAAACAGCATGATATTCGA
  TCATTTTTTGTACCACAACCTAAAAAAAGACAGTTGATGACCTCCTGTGACGAATCAAAGAGATTCCGG
  GAGGAAAATACTGTAGTGTCATCAGACCCTACAAAAACAGCTGCAAGAGATATCATCGATTATGAAAGT
  GATGTTGAACCTGAAACGAAAAGATTGAAATTGGCTGCCTCGGAAGACCACTGCAGTCCGTCGGAAGAG
  ACACCATCCCAGTCCAAGCAAATCCGAACTCCACTCGTGGAAAGTGTACAGGAGGCCAAGGCCCAGTTA
  ACCACCCCAGCCTTTCCTGTAGAGGGCTGGCAATGTAGTCTCTGCACCTATATCAATAATTCAGAGTTA
  CCTTATTGTGAAATGTGTGAGACTCCTCAAGGCAGTGCTGTTATGCAAATAGATAGCCTCAACCATATC
  CAGGATAAAAACGAGAAGGATGATTCTCAGAAAGACACCTCCAAAAAGGTTCAAACTATCTCAGACTGT
  GAAAAACAAGCCCTTGCACAGTCAGAACCTGGCCAGTTGGCTGACAGCAAGGAAGAAACACCAAAAATT
  GAGAAAGAAGACGGACTTACATCCCAGCCAGGTAATGAACAGTGGAAGAGTTCAGACACTTTGCCAGTG
  TATGACACCTTAATGTTCTGTGCAAGTAGGAATACTGACCGGATTCACATCTATACTAAGGATGGAAAA
  CAGATGAGCTGTAATTTCATTCCTCTGGATATAAAATTAGACCTTTGGGAAGATTTACCAGCAAGCTTT
  CAGCTGAAACAATATCGCTCACTGATTTTGAGATTTGTTCGAGAATGGAGTAGTCTAACTGCCATGAAG
  CAAAGGATAATCAGGAAAAGTGGACAGCTATTCTGTAGCCCAATTCTTGCTTTGGAAGAGATCACAAAG
  CAACAAACCAAACAAAATTGCACCAAAAGATACATAACCAAAGAAGATGTTGCCGTAGCCTCAATGGAC
  AAAGTGAAGAATGTTGGGGGCCATGTCCGTCTGATCACAAAGGAGTCCAGGCCACGGGATCCTTTCACA
  AAAAAACTTCTTGAAGATGGAGCCTGTGTCCCATTTCTAAATCCATACACAGTCCAGGCAGATCTCACT
  GTGAAGCCCTCTACATCCAAAGGCTATTTGCAAGCTGTGGATAATGAAGGAAATCCACTTTGCCTTCGC
  TGTCAGCAACCCACTTGCCAGACTAAGCAAGCATGTAAAGCGAACTCTTGGGATTCACGGTTTTGCTCT
  CTGAAATGTCAGGAAGAGTTTTGGATTCGATCTAATAACAGTTACCTGAGAGCCAAAGTATTTGAAACT
  GAACATGGTGTGTGTCAGCTCTGTAATGTGAACGCACAAGAACTCTTTTTACGTCTGAGAGATGCCCCT
  AAAAGTCAGAGGAAGAATCTTCTGTATGCTACCTGGACTTCAAAGCTCCCATTAGAACAGCTAAATGAA
  ATGATAAGAAACCCAGGGGAAGGACATTTCTGGCAGGTGGATCACATCAAGCCAGTGTATGGGGGAGGA
  GGACAGTGTTCCCTGGACAACCTGCAGACTCTCTGCACAGTCTGTCACAAAGAGAGAACTGCCAGACAA
  GCTAAGGAAAGAAGCCAGGTGAGAAGACAATCTCTAGCATCAAAGCATGGATCAGACATCACACGATTT
  TTGGTAAAGAAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239134
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MWGMLNRKAQVTGSTLNGRKEKIQAEEGDKEKWDFLQFAEAWTPNDSSEELRKEALFTHFEKEKQHDIR
  SFFVPQPKKRQLMTSCDESKRFREENTVVSSDPTKTAARDIIDYESDVEPETKRLKLAASEDHCSPSEE
  TPSQSKQIRTPLVESVQEAKAQLTTPAFPVEGWQCSLCTYINNSELPYCEMCETPQGSAVMQIDSLNHI
  QDKNEKDDSQKDTSKKVQTISDCEKQALAQSEPGQLADSKEETPKIEKEDGLTSQPGNEQWKSSDTLPV
  YDTLMFCASRNTDRIHIYTKDGKQMSCNFIPLDIKLDLWEDLPASFQLKQYRSLILRFVREWSSLTAMK
  QRIIRKSGQLFCSPILALEEITKQQTKQNCTKRYITKEDVAVASMDKVKNVGGHVRLITKESRPRDPFT
  KKLLEDGACVPFLNPYTVQADLTVKPSTSKGYLQAVDNEGNPLCLRCQQPTCQTKQACKANSWDSRFCS
  LKCQEEFWIRSNNSYLRAKVFETEHGVCQLCNVNAQELFLRLRDAPKSQRKNLLYATWTSKLPLEQLNE
  MIRNPGEGHFWQVDHIKPVYGGGGQCSLDNLQTLCTVCHKERTARQAKERSQVRRQSLASKHGSDITRF
  LVKK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286569
• ORF Size: 1875 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001286569.1, NP_001273498.1
• RefSeq Size: 6958 bp
• RefSeq ORF: 1878 bp
• Locus ID: 84083
• Cytogenetics: 2q21.3
• Protein Families: Druggable Genome
• MW: 72 kDa
• Gene Summary: DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events (PubMed:21078962, PubMed:22704558, PubMed:22705370, PubMed:22759634, PubMed:26884333). Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase (PubMed:22759634). In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (PubMed:22705370, PubMed:22704558).[UniProtKB/Swiss-Prot Function]