ZRANB3 (NM_001286569) Human Tagged ORF Clone

CAT#: RC239134

ZRANB3 (myc-DDK-tagged) - Human zinc finger, RAN-binding domain containing 3 (ZRANB3), transcript variant 3

Product Images

Circular map for RC239134

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZRANB3
• Synonyms: 4933425L19Rik; AH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239134 representing NM_001286569
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGGAATGTTGAATCGCAAGGCTCAAGTTACAGGGAGCACACTGAACGGTAGGAAAGAAAAAATTC
  AGGCTGAGGAAGGTGATAAGGAAAAATGGGATTTCCTGCAGTTTGCTGAAGCTTGGACTCCAAATGACAG
  TTCTGAAGAGTTAAGGAAGGAAGCTTTGTTCACTCACTTCGAAAAAGAAAAACAGCATGATATTCGATCA
  TTTTTTGTACCACAACCTAAAAAAAGACAGTTGATGACCTCCTGTGACGAATCAAAGAGATTCCGGGAGG
  AAAATACTGTAGTGTCATCAGACCCTACAAAAACAGCTGCAAGAGATATCATCGATTATGAAAGTGATGT
  TGAACCTGAAACGAAAAGATTGAAATTGGCTGCCTCGGAAGACCACTGCAGTCCGTCGGAAGAGACACCA
  TCCCAGTCCAAGCAAATCCGAACTCCACTCGTGGAAAGTGTACAGGAGGCCAAGGCCCAGTTAACCACCC
  CAGCCTTTCCTGTAGAGGGCTGGCAATGTAGTCTCTGCACCTATATCAATAATTCAGAGTTACCTTATTG
  TGAAATGTGTGAGACTCCTCAAGGCAGTGCTGTTATGCAAATAGATAGCCTCAACCATATCCAGGATAAA
  AACGAGAAGGATGATTCTCAGAAAGACACCTCCAAAAAGGTTCAAACTATCTCAGACTGTGAAAAACAAG
  CCCTTGCACAGTCAGAACCTGGCCAGTTGGCTGACAGCAAGGAAGAAACACCAAAAATTGAGAAAGAAGA
  CGGACTTACATCCCAGCCAGGTAATGAACAGTGGAAGAGTTCAGACACTTTGCCAGTGTATGACACCTTA
  ATGTTCTGTGCAAGTAGGAATACTGACCGGATTCACATCTATACTAAGGATGGAAAACAGATGAGCTGTA
  ATTTCATTCCTCTGGATATAAAATTAGACCTTTGGGAAGATTTACCAGCAAGCTTTCAGCTGAAACAATA
  TCGCTCACTGATTTTGAGATTTGTTCGAGAATGGAGTAGTCTAACTGCCATGAAGCAAAGGATAATCAGG
  AAAAGTGGACAGCTATTCTGTAGCCCAATTCTTGCTTTGGAAGAGATCACAAAGCAACAAACCAAACAAA
  ATTGCACCAAAAGATACATAACCAAAGAAGATGTTGCCGTAGCCTCAATGGACAAAGTGAAGAATGTTGG
  GGGCCATGTCCGTCTGATCACAAAGGAGTCCAGGCCACGGGATCCTTTCACAAAAAAACTTCTTGAAGAT
  GGAGCCTGTGTCCCATTTCTAAATCCATACACAGTCCAGGCAGATCTCACTGTGAAGCCCTCTACATCCA
  AAGGCTATTTGCAAGCTGTGGATAATGAAGGAAATCCACTTTGCCTTCGCTGTCAGCAACCCACTTGCCA
  GACTAAGCAAGCATGTAAAGCGAACTCTTGGGATTCACGGTTTTGCTCTCTGAAATGTCAGGAAGAGTTT
  TGGATTCGATCTAATAACAGTTACCTGAGAGCCAAAGTATTTGAAACTGAACATGGTGTGTGTCAGCTCT
  GTAATGTGAACGCACAAGAACTCTTTTTACGTCTGAGAGATGCCCCTAAAAGTCAGAGGAAGAATCTTCT
  GTATGCTACCTGGACTTCAAAGCTCCCATTAGAACAGCTAAATGAAATGATAAGAAACCCAGGGGAAGGA
  CATTTCTGGCAGGTGGATCACATCAAGCCAGTGTATGGGGGAGGAGGACAGTGTTCCCTGGACAACCTGC
  AGACTCTCTGCACAGTCTGTCACAAAGAGAGAACTGCCAGACAAGCTAAGGAAAGAAGCCAGGTGAGAAG
  ACAATCTCTAGCATCAAAGCATGGATCAGACATCACACGATTTTTGGTAAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239134 representing NM_001286569
  Red=Cloning site Green=Tags(s)
  
  MWGMLNRKAQVTGSTLNGRKEKIQAEEGDKEKWDFLQFAEAWTPNDSSEELRKEALFTHFEKEKQHDIRS
  FFVPQPKKRQLMTSCDESKRFREENTVVSSDPTKTAARDIIDYESDVEPETKRLKLAASEDHCSPSEETP
  SQSKQIRTPLVESVQEAKAQLTTPAFPVEGWQCSLCTYINNSELPYCEMCETPQGSAVMQIDSLNHIQDK
  NEKDDSQKDTSKKVQTISDCEKQALAQSEPGQLADSKEETPKIEKEDGLTSQPGNEQWKSSDTLPVYDTL
  MFCASRNTDRIHIYTKDGKQMSCNFIPLDIKLDLWEDLPASFQLKQYRSLILRFVREWSSLTAMKQRIIR
  KSGQLFCSPILALEEITKQQTKQNCTKRYITKEDVAVASMDKVKNVGGHVRLITKESRPRDPFTKKLLED
  GACVPFLNPYTVQADLTVKPSTSKGYLQAVDNEGNPLCLRCQQPTCQTKQACKANSWDSRFCSLKCQEEF
  WIRSNNSYLRAKVFETEHGVCQLCNVNAQELFLRLRDAPKSQRKNLLYATWTSKLPLEQLNEMIRNPGEG
  HFWQVDHIKPVYGGGGQCSLDNLQTLCTVCHKERTARQAKERSQVRRQSLASKHGSDITRFLVKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286569
• ORF Size: 1875 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286569.1, NP_001273498.1
• RefSeq Size: 6958 bp
• RefSeq ORF: 1878 bp
• Locus ID: 84083
• Cytogenetics: 2q21.3
• Protein Families: Druggable Genome
• MW: 72 kDa
• Gene Summary: DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events (PubMed:21078962, PubMed:22704558, PubMed:22705370, PubMed:22759634, PubMed:26884333). Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase (PubMed:22759634). In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (PubMed:22705370, PubMed:22704558).[UniProtKB/Swiss-Prot Function]