NSUN2 (NM_001193455) Human Tagged ORF Clone

CAT#: RG231394

NSUN2 (tGFP-tagged) - Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 2

Product Images

Circular map for RG231394

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NSUN2
• Synonyms: MISU; MRT5; SAKI; TRM4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231394 representing NM_001193455
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGGCGGTCGCGGGGTCGGCGGCTCCAGCAACAGCAGCGGCCGGAGGACGCGGAGGATGGCGCCG
  AGGGTGGTGGAAAGCGCGGCGAGGCGGGCTGGGAAGGAGGCTACCCCGAGATCGTCAAGGAGAACAAGCT
  GTTCGAGCACTACTACCAGGAGCTCAAGATCGTGCCCGAGGGCGAGTGGGGCCAGTTCATGGACGCTCTC
  AGGGAGCCGCTCCCGGCCACTTTAAGAATTACTGGTTACAAAAGGTATCCTGAAGAACTTGCCTGGCACA
  CAAATTTAAGTCGAAAAATCTTGAGAAAATCGCCACACTTGGAAAAGTTTCATCAGTTTCTAGTTAGTGA
  AACAGAATCTGGAAATATTAGTCGTCAAGAAGCTGTTAGCATGATCCCACCACTGCTCCTCAACGTGCGG
  CCTCATCATAAGATCTTAGATATGTGTGCAGCACCTGGCTCAAAGACCACACAGTTAATTGAAATGCTAC
  ATGCCGACATGAATGTCCCCTTTCCAGAGGGATTTGTTATTGCGAATGATGTGGACAACAAGCGCTGCTA
  CCTGCTCGTCCATCAAGCCAAGAGGCTGAGCAGCCCCTGCATCATGGTGGTCAACCATGATGCCTCCAGC
  ATACCCAGGCTCCAGATAGATGTGGACGGCAGGAAAGAGATCCTCTTCTATGATCGAATTTTATGTGATG
  TCCCTTGCAGTGGAGACGGCACTATGAGAAAAAACATTGATGTTTGGAAAAAGTGGACCACCTTAAATAG
  CTTGCAGCTACATGGCTTACAGCTGCGGATTGCAACACGCGGGGCTGAACAGCTGGCTGAAGGTGGAAGG
  ATGGTGTATTCCACGTGTTCACTAAACCCTATTGAGGATGAAGCAGTCATAGCATCTTTACTGGAAAAAA
  GTGAAGGTGCTTTGGAGCTTGCTGATGTGTCTAATGAACTGCCAGGGCTGAAGTGGATGCCTGGAATCAC
  ACAGTGGAAGGTAATGACGAAAGATGGGCAGTGGTTTACAGACTGGGACGCTGTTCCTCACAGCAGACAC
  ACCCAGATCCGACCTACCATGTTCCCTCCGAAGGACCCAGAAAAGCTGCAGGCCATGCACCTGGAGCGAT
  GCCTTAGGATATTACCCCATCATCAGAATACTGGAGGGTTTTTTGTGGCAGTATTGGTGAAAAAATCTTC
  AATGCCGTGGAATAAACGTCAGCCAAAGCTTCAGGGTAAATCTGCAGAGACCAGAGAAAGCACACAGCTG
  AGCCCTGCAGATCTCACAGAAGGGAAACCCACAGATCCCTCTAAGCTGGAAAGTCCGTCATTCACAGGAA
  CTGGTGACACAGAAATAGCTCATGCAACTGAGGATTTAGAGAATAATGGCAGTAAGAAAGATGGCGTGTG
  TGGTCCTCCTCCATCAAAGAAAATGAAGTTATTTGGATTTAAAGAAGATCCATTTGTATTTATTCCTGAA
  GATGACCCATTATTTCCACCTATTGAGAAATTTTATGCTTTGGATCCTTCATTCCCAAGGATGAATTTGT
  TAACTCGGACTACAGAAGGGAAGAAAAGGCAGCTCTACATGGTTTCTAAGGAGTTGCGGAATGTGCTGCT
  GAATAACAGTGAGAAGATGAAGGTTATTAACACGGGGATCAAAGTCTGGTGTAGAAATAACAGCGGTGAA
  GAGTTTGACTGTGCTTTCCGGCTGGCACAGGAGGGAATATATACATTGTATCCATTTATTAACTCAAGAA
  TTATTACTGTATCAATGGAAGATGTTAAGATACTGTTGACCCAGGAAAATCCCTTTTTTAGAAAACTCAG
  CAGTGAGACCTACAGTCAAGCAAAGGACCTGGCAAAGGGAAGCATCGTGCTGAAGTATGAACCAGATTCT
  GCGAATCCAGACGCTCTGCAGTGTCCCATCGTCTTATGCGGATGGCGGGGAAAGGCCTCCATTCGAACTT
  TTGTGCCCAAGAATGAACGGCTTCATTATCTCAGGATGATGGGGCTGGAGGTATTGGGAGAAAAGAAGAA
  GGAAGGGGTTATCCTCACAAATGAGAGTGCAGCCAGCACCGGACAGCCAGACAATGACGTGACTGAGGGA
  CAGAGAGCAGGAGAGCCCAACAGCCCAGATGCAGAAGAGGCCAACAGTCCAGACGTGACAGCAGGCTGTG
  ACCCGGCGGGGGTCCATCCACCCCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231394 representing NM_001193455
  Red=Cloning site Green=Tags(s)
  
  MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWGQFMDAL
  REPLPATLRITGYKRYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVR
  PHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASS
  IPRLQIDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGR
  MVYSTCSLNPIEDEAVIASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRH
  TQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQL
  SPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPE
  DDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGE
  EFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDS
  ANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEG
  QRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001193455
• ORF Size: 2196 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001193455.2
• RefSeq Size: 3284 bp
• RefSeq ORF: 2199 bp
• Locus ID: 54888
• UniProt ID: Q08J23
• Cytogenetics: 5p15.31
• Gene Summary: This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011]