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FLG2 (NM_001014342) Human Tagged ORF Clone
CAT#: RG214428
- TrueORF®
FLG2 (tGFP-tagged) - Human filaggrin family member 2 (FLG2). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_001014342" in other vectors (2)
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Other products for "FLG2"
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | FLG2 |
| Synonyms | IFPS; PSS6 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214428 ORF sequence, codon optimized.
Due to the complexity of NM_001014342, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGACCTCCTGCGGAGCGTGGTGACGGTGATTGACGTTTTTTACAAGTACACCAAGCAAGATGGAG AATGTGGAACACTGTCAAAGGGTGAGCTGAAGGAGCTCCTGGAAAAGGAACTTCATCCAGTATTGAAGAA CCCAGACGACCCGGATACGGTGGACGTGATTATGCACATGCTGGACAGAGATCATGACAGGCGCTTGGAC TTTACTGAGTTCCTGTTGATGATTTTTAAGCTGACAATGGCATGTAACAAGGTGTTGAGTAAAGAGTACT GCAAGGCTTCAGGGAGCAAAAAGCATCGCCGGGGGCATAGACATCAGGAGGAAGAATCAGAAACAGAAGA GGATGAGGAGGACACACCTGGGCATAAGAGTGGCTATAGACACAGCTCTTGGTCCGAGGGCGAGGAACAC GGCTATTCCTCAGGTCATAGCCGCGGTACAGTGAAATGCAGACACGGATCTAATTCTCGACGGCTTGGGC GGCAGGGCAACCTGTCTAGTTCTGGGAATCAGGAAGGATCACAAAAGCGGTATCATCGGAGTTCTTGTGG CCACAGCTGGAGCGGGGGGAAGGACCGCCACGGTTCCAGCTCCGTGGAGTTGCGAGAGAGGATCAATAAA AGCCACATCAGCCCAAGCCGCGAGTCTGGCGAGGAATACGAGAGCGGGTCAGGAAGCAATAGCTGGGAGA GAAAGGGCCACGGAGGCCTTTCCTGTGGATTGGAGACGTCCGGTCACGAGAGCAATTCAACCCAGAGTCG GATTCGGGAGCAGAAACTGGGTTCTTCTTGCTCCGGCAGTGGCGACAGTGGCCGGCGGTCACATGCTTGT GGGTATAGCAACAGCTCTGGTTGCGGGAGGCCTCAAAATGCCTCTTCATCCTGTCAATCTCACCGGTTCG GCGGACAGGGAAACCAGTTCTCCTATATTCAGAGTGGGTGCCAGTCCGGCATCAAAGGCGGCCAGGGACA CGGTTGCGTGAGTGGGGGTCAGCCCTCAGGCTGCGGGCAGCCAGAGTCAAACCCATGCTCACAGTCATAC TCTCAAAGGGGCTATGGTGCCCGAGAGAATGGACAGCCCCAGAACTGTGGTGGCCAGTGGCGAACTGGGA GCAGTCAGAGCTCATGCTGCGGACAGTATGGCAGCGGCGGCTCTCAGTCATGCTCCAACGGCCAGCACGA ATATGGCTCTTGCGGTCGCTTTTCTAACAGTTCAAGCTCCAACGAGTTTTCAAAATGCGATCAATACGGC TCCGGTAGCTCTCAGAGTACCTCTTTTGAACAGCATGGCACAGGTCTGTCCCAGAGTAGTGGATTCGAGC AGCATGTCTGTGGATCCGGCCAGACCTGTGGACAGCATGAGTCAACTTCTTCTCAGAGCCTTGGTTACGA CCAGCACGGAAGTTCAAGTGGGAAAACTTCTGGATTCGGACAACACGGTAGCGGATCCGGACAAAGCAGT GGCTTTGGCCAGTGCGGTAGCGGCTCCGGCCAGAGCTCTGGTTTTGGCCAGCACGGGTCTGTGTCAGGCC AGAGCTCTGGATTCGGCCAGCATGGTTCCGTGTCCGGCCAGAGCTCTGGATTCGGTCAGCACGAGTCAAG ATCTAGACAGTCCTCTTATGGGCAACACGGGTCTGGTTCTTCCCAGTCCTCCGGGTATGGTCAATACGGG TCCAGGGAGACTTCCGGATTCGGGCAGCATGGCCTGGGCAGTGGTCAGAGTACCGGCTTTGGCCAATACG GGTCAGGGAGCGGCCAGTCAAGCGGGTTCGGTCAGCATGGCAGCGGCTCTGGACAGTCAAGCGGATTTGG GCAGCACGAGTCACGCAGTGGTCAGAGTAGTTATGGCCAGCATTCCTCCGGTAGTTCACAGTCCAGCGGA TACGGGCAGCACGGGAGCCGGCAAACCAGCGGTTTTGGCCAGCATGGAAGCGGCAGCAGCCAGAGTACTG GCTTCGGACAGTACGGATCAGGTTCTGGACAATCCTCAGGATTCGGGCAGCATGTGTCTGGCTCTGGCCA ATCATCTGGCTTTGGACAGCACGAATCCCGCAGTGGTCACTCTAGTTACGGTCAGCACGGATTTGGTAGC TCCCAGAGTTCAGGGTATGGCCAGCATGGATCTAGCTCTGGCCAGACATCTGGTTTTGGGCAACACGAAC TGTCATCTGGCCAATCCTCATCTTTTGGCCAACACGGCAGTGGTTCAGGACAGTCTTCCGGATTCGGACA ACACGGGTCAGGCTCTGGACAGTCTAGTGGCTTTGGCCAGCATGAGTCCCGAAGTGGGCAATCCAGCTAT GGCCAGCATTCCAGCGGGAGCTCCCAAAGCAGTGGCTACGGTCAACACGGCTCCAGACAGACTAGCGGGT TCGGGCAGCATGGCTCTGGCAGTAGCCAAAGTACGGGCTTTGGCCAATACGGCAGTGGAAGCGGACAATC AGCTGGTTTTGGCCAGCATGGCTCTGGCAGTGGGCAGTCCAGTGGCTTCGGCCAGCACGAGTCTCGCTCC CACCAGAGTTCTTACGGACAACATGGGTCAGGCAGCAGTCAGTCCTCCGGCTATGGCCAGCATGGGAGCT CCTCCGGCCAAACGTCCGGATTTGGCCAGCATCGGAGCAGCTCTGGGCAGTACAGCGGTTTTGGTCAGCA CGGCTCCGGTTCCGGCCAGAGCAGCGGCTTTGGCCAGCACGGGACCGGGTCAGGGCAGTATTCTGGCTTC GGCCAGCATGAGTCTAGGAGTCACCAAAGCTCTTACGGCCAACATGGTTCAGGATCATCTCAGTCATCCG GATATGGGCAGCATGGAAGTTCAAGCGGTCAGACTTTCGGCTTCGGTCAGCATAGGTCTGGGAGTGGCCA GTCTTCTGGATTCGGACAGCATGGCTCAGGAAGTGGACAAAGCAGCGGGTTCGGACAGCACGAGAGCGGC TCAGGAAAATCCTCTGGATTTGGCCAGCACGAATCCAGGTCATCACAGTCAAACTATGGCCAGCACGGGA GCGGTTCCAGCCAGTCATCTGGCTATGGTCAGCATGGCTCAAGCTCTGGGCAAACGACCGGATTCGGCCA ACACAGGAGCAGCAGTGGCCAGTATTCCGGCTTCGGGCAGCATGGGTCAGGCAGCGACCAATCATCCGGC TTCGGGCAGCACGGAACAGGGAGCGGTCAATCATCCGGCTTCGGCCAATACGAGTCCCGGTCTAGGCAGT CCTCTTATGGACAACACGGGTCTGGCTCCAGCCAAAGCAGCGGTTACGGCCAGCATGGAAGTAACTCAGG GCAAACCTCCGGATTTGGCCAGCACCGGCCAGGTAGCGGGCAAAGTAGCGGCTTTGGGCAGTACGGCTCT GGATCAGGACAGTCAAGCGGCTTCGGCCAACACGGCTCAGGGACAGGCAAGAGCTCCGGTTTCGCTCAAC ACGAGTATAGGTCTGGTCAGAGTTCCTATGGACAGCACGGGACAGGATCCTCTCAATCCTCAGGATGTGG GCAGCACGAATCAGGTTCCGGGCCGACCACATCTTTTGGCCAGCATGTGTCTGGCTCTGACAACTTTTCT TCTTCAGGCCAGCACATCTCAGACTCAGGCCAATCCACGGGATTCGGTCAATATGGATCAGGGAGTGGGC AGAGCACGGGACTGGGTCAAGGCGAGTCTCAACAGGTGGAGAGCGGTAGCACTGTGCACGGACGGCAGGA AACTACACATGGACAGACCATAAACACAACACGCCACAGCCAATCTGGCCAGGGACAGTCTACACAGACG GGATCCCGGGTGACCAGAAGAAGAAGAAGTAGCCAGTCCGAGAATAGTGACAGTGAGGTCCACAGCAAGG TGTCACACCGGCACAGTGAGCATATACACACCCAGGCTGGGTCACACTACCCCAAGTCAGGATCTACTGT TCGAAGGAGGCAGGGGACCACACATGGACAAAGAGGGGATACAACACGACATGGCCACTCTGGACACGGT CAGAGCACACAGACAGGGAGTCGGACATCTGGAAGGCAGAGGTTTTCTCACTCTGATGCCACCGACTCCG AAGTGCATAGCGGGGTATCCCATAGACCGCATTCTCAGGAGCAGACCCATTCACAGGCAGGAAGCCAGCA CGGTGAATCCGAAAGCACCGTACATGAAAGACACGAAACCACGTATGGTCAAACCGGCGAAGCAACGGGT CACGGACATAGTGGCCACGGCCAGTCAACCCAGAGAGGGAGCCGCACCACCGGGCGGCGGGGGTCTGGAC ATTCCGAATCCAGCGATTCCGAGGTCCACTCTGGAGGAAGCCACAGACCACAGTCCCAAGAGCAGACCCA CGGCCAGGCTGGATCTCAGCACGGGGAGTCTGGTAGTACCGTCCACGGGAGGCATGGAACAACACACGGC CAAACAGGGGACACAACGCGGCACGCACACTACCACCATGGCAAGAGCACACAGAGGGGCTCTTCAACCA CTGGCAGACGGGGTAGTGGGCACAGCGAATCTAGCGACTCCGAAGTGCACTCCGGTGGGAGCCATACACA CAGCGGGCATACTCATGGGCAAAGTGGCTCCCAGCATGGGGAAAGCGAAAGTATTATACACGACAGGCAC CGGATCACGCACGGTCAGACCGGTGATACTACGCGGCACTCCTATAGTGGACACGAGCAGACAACACAGA CAGGAAGCCGCACGACAGGAAGACAGCGGACAAGCCACTCTGAGAGCACTGATAGTGAGGTGCATAGTGG CGGATCCCACAGGCCGCACTCACGCGAACACACCTATGGACAGGCAGGTAGTCAGCACGAGGAGCCTGAG TTCACCGTTCACGAGCGACATGGGACTACACACGGCCAGATCGGAGACACTACGGGGCACAGCCACAGTG GACACGGCCAAAGCACTCAGAGGGGTTCTAGGACCACAGGAAGACAGAGATCAAGTCATTCCGAGAGTTC CGACTCTGAGGTCCACTCAGGTGTCAGCCACACGCACACCGGTCACACCCATGGGCAGGCAGGGAGCCAG CACGGGCAGTCTGAGAGCATCGTACCTGAACGACATGGTACCACACATGGCCAGACAGGCGACACTACTC GGCACGCACATTACCACCACGGCCTGACCACTCAGACCGGTAGCCGGACCACAGGACGGAGGGGTTCCGG ACACTCAGAGTATAGCGATTCAGAGGGGTATAGCGGCGTCTCCCATACCCACTCCGGGCATACTCATGGG CAGGCCCGCAGTCAGCATGGCGAATCAGAATCTATCGTCCATGAACGACACGGCACAATCCATGGGCAGA CCGGCGACACAACTCGACATGCCCACTCTGGCCACGGCCAGAGCACTCAGACTGGCAGCAGGACAACCGG TCGGAGAAGCAGCGGCCATTCCGAGTATAGCGACAGCGAGGGTCACAGCGGCTTCAGCCAGAGGCCTCAC TCCCGAGGACATACACACGGACAAGCCGGTAGCCAGCACGGAGAGTCCGAGAGCATAGTCGATGAGAGGC ACGGAACCACACATGGACAAACAGGCGACACTTCCGGACATAGCCAGAGCGGGCACGGCCAGAGTACCCA GTCTGGTAGCAGCACAACAGGAAGGCGGCGGAGTGGACACTCCGAGTCATCAGATTCAGAGGTGCATTCA GGGGGTTCTCACACACACTCCGGCCACACACACAGCCAGGCCAGGTCACAGCACGGCGAGTCAGAAAGCA CGGTCCACAAGAGACATCAAACCACTCATGGGCAGACTGGCGATACAACAGAGCATGGACACCCATCCCA TGGACAAACCATACAGACTGGAAGCCGGACCACCGGTAGGAGAGGTTCTGGCCACTCAGAGTATTCCGAC TCTGAGGGCCCCAGCGGCGTAAGCCATACACATAGCGGACACACTCACGGCCAGGCCGGTTCACATTATC CAGAATCAGGAAGCAGTGTGCATGAACGGCATGGGACTACCCATGGCCAGACTGCTGATACTACAAGACA TGGCCATTCCGGCCATGGACAGTCTACCCAGAGGGGGAGCCGCACGACAGGAAGGAGAGCCTCTGGGCAC TCAGAGTACAGTGACAGCGAAGGACACTCAGGCGTGAGTCACACTCACTCCGGCCATGCCCACGGCCAGG CTGGTAGTCAACACGGAGAGTCTGGGTCATCCGTGCACGAGAGGCACGGCACAACGCACGGCCAGACAGG TGATACCACAAGGCATGCTCACAGTGGCCACGGCCAGTCAACACAGAGGGGGTCTAGAACTGCGGGCCGG CGCGGATCCGGGCACTCCGAAAGCTCCGATAGCGAGGTGCACAGTGGCGTGAGCCATACCCACAGCGGAC ACACCTACGGCCAAGCCCGCAGCCAGCACGGAGAATCCGGGAGTGCAATTCATGGCCGCCAGGGTACGAT TCACGGACAGACAGGCGATACTACGCGCCATGGACAATCTGGACACGGTCAGTCTACTCAAACTGGAAGC AGGACCACAGGCAGACAGCGCAGCAGCCATAGTGAAAGTAGCGACTCAGAAGTACACAGTGAGGCCAGCC CAACCCACAGTGGCCACACACATTCCCAAGCCGGAAGCCGGCACGGACAGAGCGGCAGCTCCGGTCATGG GCGCCAGGGCACGACTCATGGTCAGACAGGTGATACTACCAGGCACGCCCATTATGGTTATGGGCAGTCT ACTCAGAGGGGGTCCAGAACGACTGGGCGCCGAGGATCAGGCCACTCAGAGTCCAGTGATTCTGAGGTTC ATTCATGGGGATCCCACACACATAGTGGCCACATCCAGGGACAGGCAGGTAGTCAACAGAGGCAGCCCGG CTCTACAGTACACGGCAGGCTCGAAACAACCCATGGACAGACTGGCGATACGACCCGCCACGGGCACTCC GGCTACGGGCAGTCAACTCAAACAGGCTCAAGAAGTTCCAGAGCCTCTCACTTCCAGAGTCATTCATCCG AGCGACAGAGACATGGCTCCTCCCAGGTGTGGAAACACGGATCATATGGCCCCGCAGAATATGATTACGG CCATACAGGATACGGCCCTTCTGGGGGATCTCGGAAATCCATCTCAAACTCCCACCTTTCTTGGTCCACG GATAGTACCGCGAATAAGCAACTCTCCCGACAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214428 representing NM_001014342
Red=Cloning site Green=Tags(s) MTDLLRSVVTVIDVFYKYTKQDGECGTLSKGELKELLEKELHPVLKNPDDPDTVDVIMHMLDRDHDRRLD FTEFLLMIFKLTMACNKVLSKEYCKASGSKKHRRGHRHQEEESETEEDEEDTPGHKSGYRHSSWSEGEEH GYSSGHSRGTVKCRHGSNSRRLGRQGNLSSSGNQEGSQKRYHRSSCGHSWSGGKDRHGSSSVELRERINK SHISPSRESGEEYESGSGSNSWERKGHGGLSCGLETSGHESNSTQSRIREQKLGSSCSGSGDSGRRSHAC GYSNSSGCGRPQNASSSCQSHRFGGQGNQFSYIQSGCQSGIKGGQGHGCVSGGQPSGCGQPESNPCSQSY SQRGYGARENGQPQNCGGQWRTGSSQSSCCGQYGSGGSQSCSNGQHEYGSCGRFSNSSSSNEFSKCDQYG SGSSQSTSFEQHGTGLSQSSGFEQHVCGSGQTCGQHESTSSQSLGYDQHGSSSGKTSGFGQHGSGSGQSS GFGQCGSGSGQSSGFGQHGSVSGQSSGFGQHGSVSGQSSGFGQHESRSRQSSYGQHGSGSSQSSGYGQYG SRETSGFGQHGLGSGQSTGFGQYGSGSGQSSGFGQHGSGSGQSSGFGQHESRSGQSSYGQHSSGSSQSSG YGQHGSRQTSGFGQHGSGSSQSTGFGQYGSGSGQSSGFGQHVSGSGQSSGFGQHESRSGHSSYGQHGFGS SQSSGYGQHGSSSGQTSGFGQHELSSGQSSSFGQHGSGSGQSSGFGQHGSGSGQSSGFGQHESRSGQSSY GQHSSGSSQSSGYGQHGSRQTSGFGQHGSGSSQSTGFGQYGSGSGQSAGFGQHGSGSGQSSGFGQHESRS HQSSYGQHGSGSSQSSGYGQHGSSSGQTSGFGQHRSSSGQYSGFGQHGSGSGQSSGFGQHGTGSGQYSGF GQHESRSHQSSYGQHGSGSSQSSGYGQHGSSSGQTFGFGQHRSGSGQSSGFGQHGSGSGQSSGFGQHESG SGKSSGFGQHESRSSQSNYGQHGSGSSQSSGYGQHGSSSGQTTGFGQHRSSSGQYSGFGQHGSGSDQSSG FGQHGTGSGQSSGFGQYESRSRQSSYGQHGSGSSQSSGYGQHGSNSGQTSGFGQHRPGSGQSSGFGQYGS GSGQSSGFGQHGSGTGKSSGFAQHEYRSGQSSYGQHGTGSSQSSGCGQHESGSGPTTSFGQHVSGSDNFS SSGQHISDSGQSTGFGQYGSGSGQSTGLGQGESQQVESGSTVHGRQETTHGQTINTTRHSQSGQGQSTQT GSRVTRRRRSSQSENSDSEVHSKVSHRHSEHIHTQAGSHYPKSGSTVRRRQGTTHGQRGDTTRHGHSGHG QSTQTGSRTSGRQRFSHSDATDSEVHSGVSHRPHSQEQTHSQAGSQHGESESTVHERHETTYGQTGEATG HGHSGHGQSTQRGSRTTGRRGSGHSESSDSEVHSGGSHRPQSQEQTHGQAGSQHGESGSTVHGRHGTTHG QTGDTTRHAHYHHGKSTQRGSSTTGRRGSGHSESSDSEVHSGGSHTHSGHTHGQSGSQHGESESIIHDRH RITHGQTGDTTRHSYSGHEQTTQTGSRTTGRQRTSHSESTDSEVHSGGSHRPHSREHTYGQAGSQHEEPE FTVHERHGTTHGQIGDTTGHSHSGHGQSTQRGSRTTGRQRSSHSESSDSEVHSGVSHTHTGHTHGQAGSQ HGQSESIVPERHGTTHGQTGDTTRHAHYHHGLTTQTGSRTTGRRGSGHSEYSDSEGYSGVSHTHSGHTHG QARSQHGESESIVHERHGTIHGQTGDTTRHAHSGHGQSTQTGSRTTGRRSSGHSEYSDSEGHSGFSQRPH SRGHTHGQAGSQHGESESIVDERHGTTHGQTGDTSGHSQSGHGQSTQSGSSTTGRRRSGHSESSDSEVHS GGSHTHSGHTHSQARSQHGESESTVHKRHQTTHGQTGDTTEHGHPSHGQTIQTGSRTTGRRGSGHSEYSD SEGPSGVSHTHSGHTHGQAGSHYPESGSSVHERHGTTHGQTADTTRHGHSGHGQSTQRGSRTTGRRASGH SEYSDSEGHSGVSHTHSGHAHGQAGSQHGESGSSVHERHGTTHGQTGDTTRHAHSGHGQSTQRGSRTAGR RGSGHSESSDSEVHSGVSHTHSGHTYGQARSQHGESGSAIHGRQGTIHGQTGDTTRHGQSGHGQSTQTGS RTTGRQRSSHSESSDSEVHSEASPTHSGHTHSQAGSRHGQSGSSGHGRQGTTHGQTGDTTRHAHYGYGQS TQRGSRTTGRRGSGHSESSDSEVHSWGSHTHSGHIQGQAGSQQRQPGSTVHGRLETTHGQTGDTTRHGHS GYGQSTQTGSRSSRASHFQSHSSERQRHGSSQVWKHGSYGPAEYDYGHTGYGPSGGSRKSISNSHLSWST DSTANKQLSRH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001014342 |
| ORF Size | 7173 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001014342.1, NP_001014364.1 |
| RefSeq Size | 9170 bp |
| RefSeq ORF | 7176 bp |
| Locus ID | 388698 |
| UniProt ID | Q5D862 |
| Cytogenetics | 1q21.3 |
| Gene Summary | The filaggrin-like protein encoded by this gene is upregulated by calcium, proteolyzed by calpain 1, and is involved in epithelial homeostasis. The encoded protein is required for proper cornification in skin, with defects in this gene being associated with skin diseases. This protein also has a function in skin barrier protection. In fact, in addition to providing a physical barrier, C-terminal fragments of this protein display antimicrobial activity against P. aeruginosa and E. coli. [provided by RefSeq, Jul 2020] |
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