CRIP2 (NM_001312) Human Tagged ORF Clone

CAT#: RG205938

CRIP2 (tGFP-tagged) - Human cysteine-rich protein 2 (CRIP2)

Product Images

Circular map for RG205938

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CRIP2
• Synonyms: CRIP; CRP2; ESP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205938 representing NM_001312
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCAAATGCCCCAAGTGCGACAAGACCGTGTACTTCGCCGAGAAGGTGAGCTCCCTGGGGAAGG
  ACTGGCACAAGTTCTGCCTCAAGTGCGAGCGCTGCAGCAAGACGCTGACGCCCGGGGGCCACGCCGAGCA
  TGACGGGAAGCCGTTCTGCCACAAGCCGTGCTACGCCACCCTGTTCGGACCCAAAGGCGTGAACATCGGG
  GGCGCGGGCTCCTACATCTACGAGAAGCCCCTGGCGGAGGGGCCGCAGGTCACCGGCCCCATCGAGGTCC
  CCGCGGCCCGAGCAGAGGAGCGGAAGGCGAGCGGCCCCCCGAAGGGGCCCAGCAGAGCCTCCAGTGTCAC
  CACTTTCACCGGGGAGCCCAACACGTGCCCGCGCTGCAGCAAGAAGGTGTACTTCGCTGAGAAGGTGACG
  TCTCTGGGCAAGGATTGGCACCGGCCCTGCCTGCGCTGCGAGCGCTGCGGGAAGACACTGACCCCCGGCG
  GGCACGCGGAGCACGACGGCCAGCCCTACTGCCACAAGCCCTGCTATGGAATCCTCTTCGGACCCAAGGG
  AGTGAACACCGGTGCGGTGGGCAGCTACATCTATGACCGGGACCCCGAAGGCAAGGTCCAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205938 representing NM_001312
  Red=Cloning site Green=Tags(s)
  
  MASKCPKCDKTVYFAEKVSSLGKDWHKFCLKCERCSKTLTPGGHAEHDGKPFCHKPCYATLFGPKGVNIG
  GAGSYIYEKPLAEGPQVTGPIEVPAARAEERKASGPPKGPSRASSVTTFTGEPNTCPRCSKKVYFAEKVT
  SLGKDWHRPCLRCERCGKTLTPGGHAEHDGQPYCHKPCYGILFGPKGVNTGAVGSYIYDRDPEGKVQP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001312
• ORF Size: 624 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001312.4
• RefSeq Size: 1236 bp
• RefSeq ORF: 627 bp
• Locus ID: 1397
• UniProt ID: P52943
• Cytogenetics: 14q32.33
• Domains: LIM
• Gene Summary: This gene encodes a putative transcription factor with two LIM zinc-binding domains. The encoded protein may participate in the differentiation of smooth muscle tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]