EAF2 (NM_018456) Human Tagged ORF Clone
CAT#: RG204314
- TrueORF®
EAF2 (tGFP-tagged) - Human ELL associated factor 2 (EAF2)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_018456" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | EAF2 |
Synonyms | BM040; TRAITS; U19 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204314 representing NM_018456
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATAGCGCAGCGGGATTCTCACACCTAGACCGTCGCGAGCGGGTTCTCAAGTTAGGGGAGAGTTTCG AGAAGCAGCCGCGCTGCGCCTTCCACACTGTGCGCTATGACTTCAAACCTGCTTCTATTGACACTTCTTC TGAAGGATACCTTGAGGTTGGTGAAGGTGAACAGGTGACCATAACTCTGCCAAATATAGAAGGTTCAACT CCACCAGTAACTGTTTTCAAAGGTTCAAAAAAACCTTACTTAAAAGAATGCATTTTGATTATTAACCATG ATACTGGAGAATGTCGGCTAGAAAAACTCAGCAGCAACATCACTGTAAAAAAAACAAGAGTTGAAGGAAG CAGTAAAATTCAGTATCGTAAAGAACAACAGCAACAACAAATGTGGAATTCAGCCAGGACTCCCAATCTT GTAAAACATTCTCCATCTGAAGATAAGATGTCCCCAGCATCTCCAATAGATGATATCGAAAGAGAACTGA AGGCAGAAGCTAGTCTAATGGACCAGATGAGTAGTTGTGATAGTTCATCAGATTCCAAAAGTTCATCATC TTCAAGTAGTGAGGATAGTTCTAGTGACTCAGAAGATGAAGATTGCAAATCCTCTACTTCTGATACAGGG AATTGTGTCTCAGGACATCCTACCATGACACAGTACAGGATTCCTGATATAGATGCCAGTCATAATAGAT TTCGAGACAACAGTGGCCTTCTGATGAATACTTTAAGAAATGATTTGCAGCTGAGTGAATCAGGAAGTGA CAGTGATGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204314 representing NM_018456
Red=Cloning site Green=Tags(s) MNSAAGFSHLDRRERVLKLGESFEKQPRCAFHTVRYDFKPASIDTSSEGYLEVGEGEQVTITLPNIEGST PPVTVFKGSKKPYLKECILIINHDTGECRLEKLSSNITVKKTRVEGSSKIQYRKEQQQQQMWNSARTPNL VKHSPSEDKMSPASPIDDIERELKAEASLMDQMSSCDSSSDSKSSSSSSSEDSSSDSEDEDCKSSTSDTG NCVSGHPTMTQYRIPDIDASHNRFRDNSGLLMNTLRNDLQLSESGSDSDD TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_018456 |
ORF Size | 780 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018456.6 |
RefSeq Size | 1024 bp |
RefSeq ORF | 783 bp |
Locus ID | 55840 |
UniProt ID | Q96CJ1 |
Cytogenetics | 3q13.33 |
Gene Summary | Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204314 | EAF2 (Myc-DDK-tagged)-Human ELL associated factor 2 (EAF2) |
USD 300.00 |
|
RC204314L3 | Lenti ORF clone of Human ELL associated factor 2 (EAF2), Myc-DDK-tagged |
USD 600.00 |
|
RC204314L4 | Lenti ORF clone of Human ELL associated factor 2 (EAF2), mGFP tagged |
USD 600.00 |
|
SC304595 | EAF2 (untagged)-Human ELL associated factor 2 (EAF2) |
USD 330.00 |
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