TCHP (NM_001143852) Human Tagged ORF Clone

CAT#: RC227291

TCHP (Myc-DDK-tagged)-Human trichoplein, keratin filament binding (TCHP), transcript variant 2

Product Images

Circular map for RC227291

• 

  Western blot validation of overexpression lysate (Cat# LY428388) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227291 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TCHP
• Synonyms: TpMs
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227291 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTCCCGACGCTGCCGTCCTACTGGTGCAGCCAGCAGCGCCTGAATCAGCAGCTAGCACGACAGC
  GAGAGCAGGAGGCCCGGCTTCGGCAGCAGTGGGAGCAGAACAGCCGTTACTTCAGGATGTCTGACATCTG
  CAGCTCCAAACAGGCAGAATGGAGCTCTAAAACCTCCTACCAGCGGAGCATGCATGCCTATCAGCGGGAG
  AAGATGAAGGAGGAGAAGAGGAGGAGTCTGGAGGCCCGACGGGAAAAGCTCAGGCAGCTCATGCAGGAGG
  AGCAGGACCTGCTGGCCAGAGAACTGGAGGAGCTGAGGCTGAGCATGAACTTGCAGGAAAGAAGAATCCG
  GGAGCAGCACGGGAAGCTGAAATCAGCCAAAGAAGAGCAGAGGAAACTGATTGCTGAACAACTTTTGTAC
  GAACACTGGAAAAAGAACAACCCGAAACTTCGAGAGATGGAGCTGGACCTTCACCAGAAGCATGTCGTAA
  ACTCTTGGGAAATGCAGAAAGAAGAAAAAAAACAGCAAGAAGCCACCGCAGAGCAAGAGAACAAACGGTA
  TGAAAATGAATATGAAAGGGCCCGAAGGGAGGCGCTAGAAAGGATGAAAGCTGAAGAGGAGAGGAGGCAG
  CTGGAGGACAAGCTCCAGGCCGAGGCACTGCTGCAACAGATGGAGGAGCTGAAGCTGAAGGAGGTGGAGG
  CGACCAAACTAAAGAAGGAGCAGGAGAATCTGTTGAAGCAGCGGTGGGAGCTAGAGAGGCTGGAGGAAGA
  GCGAAAGCAGATGGAAGCCTTCCGGCAGAAGGCAGAGCTGGGGCGTTTCTTGAGACATCAGTATAACGCT
  CAACTCAGCAGACGCACACAGCAGATCCAAGAGGAGCTGGAGGCAGACAGGCGGATCCTGCAGGCCCTCC
  TCGAGAAGGAGGACGAGAGCCAGCGCCTCCACCTGGCCAGGCGGGAGCAGGTCATGGCCGATGTGGCCTG
  GATGAAGCAGGCCATTGAGGAGCAGCTGCAGCTGGAGCGGGCGCGGGAGGCAGAGCTGCAGATGCTGCTG
  AGGGAGGAGGCCAAGGAGATGTGGGAAAAGAGAGAGGCAGAGTGGGCCCGAGAGCGCAGCGCACGGGACA
  GACTGATGAGCGAGGTTCTGACAGGGAGACAACAGCAAATACAAGAGAAGATTGAGCAGAACCGACGGGC
  ACAAGAGGAATCCCTGAAACACAGGGAGCAACTTATTCGAAATCTTGAGGAGGTGAGAGAGTTGGCTCGT
  CGCGAGAAAGAGGAGAGTGAAAAGCTGAAATCGGCCAGGAAGCAGGAGCTGGAAGCCCAGGTTGCAGAGC
  GCCGGCTGCAGGCATGGGAAGCAGACCAGCAGGAGGAGGAGGAAGAGGAGGAGGCCCGGCGGGTCGAGCA
  GCTCTCAGATGCCCTGCTGCAGCAGGAGGCGGAGACTATGGCTGAGCAGGGCTACCGGCCTAAGCCTTAC
  GGACATCCAAAAATTGCTTGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227291 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALPTLPSYWCSQQRLNQQLARQREQEARLRQQWEQNSRYFRMSDICSSKQAEWSSKTSYQRSMHAYQRE
  KMKEEKRRSLEARREKLRQLMQEEQDLLARELEELRLSMNLQERRIREQHGKLKSAKEEQRKLIAEQLLY
  EHWKKNNPKLREMELDLHQKHVVNSWEMQKEEKKQQEATAEQENKRYENEYERARREALERMKAEEERRQ
  LEDKLQAEALLQQMEELKLKEVEATKLKKEQENLLKQRWELERLEEERKQMEAFRQKAELGRFLRHQYNA
  QLSRRTQQIQEELEADRRILQALLEKEDESQRLHLARREQVMADVAWMKQAIEEQLQLERAREAELQMLL
  REEAKEMWEKREAEWARERSARDRLMSEVLTGRQQQIQEKIEQNRRAQEESLKHREQLIRNLEEVRELAR
  REKEESEKLKSARKQELEAQVAERRLQAWEADQQEEEEEEEARRVEQLSDALLQQEAETMAEQGYRPKPY
  GHPKIAWN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143852
• ORF Size: 1494 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001143852.2
• RefSeq Size: 3111 bp
• RefSeq ORF: 1497 bp
• Locus ID: 84260
• UniProt ID: Q9BT92
• Cytogenetics: 12q24.11
• MW: 61.1 kDa
• Gene Summary: Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. Inhibits cell growth in bladder and prostate cancer cells by a down-regulation of HSPB1 by inhibiting its phosphorylation. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells (PubMed:15731013, PubMed:18931701). Is a negative regulator of ciliogenesis (PubMed:25270598).[UniProtKB/Swiss-Prot Function]