SPNS2 (NM_001124758) Human Tagged ORF Clone

CAT#: RC225940

SPNS2 (Myc-DDK-tagged)-Human spinster homolog 2 (Drosophila) (SPNS2)

Product Images

Circular map for RC225940

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPNS2
• Synonyms: DFNB115; SLC62A2; SLC63A2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225940 representing NM_001124758
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCCTGGAATGCGCCTCGGCGGCGGCGGGCGGCGCGGAGGAGGAGGAGGCGGACGCGGAGCGGCGGC
  GCCGGCGCCGGGGGGCGCAGCGAGGGGCTGGCGGTAGCGGTTGCTGCGGGGCGCGGGGCGCGGGCGGCGC
  TGGAGTCTCGGCCGCGGGCGATGAGGTGCAGACGCTGTCGGGCAGCGTAAGGCGGGCCCCGACCGGACCC
  CCCGGCACCCCCGGCACCCCCGGCTGCGCAGCTACTGCAAAGGGCCCCGGCGCTCAGCAGCCCAAACCGG
  CCAGCTTGGGCCGCGGGCGGGGGGCAGCCGCCGCCATCCTCAGCTTGGGCAACGTGCTCAACTACCTGGA
  CAGGTACACCGTGGCAGGCGTCCTTCTGGACATCCAGCAGCACTTTGGGGTCAAGGACCGAGGCGCCGGC
  CTGCTGCAGTCAGTGTTCATCTGTAGCTTCATGGTGGCTGCCCCCATCTTCGGCTACCTGGGCGACCGCT
  TCAACAGGAAGGTGATTCTCAGCTGCGGCATTTTCTTCTGGTCGGCCGTCACCTTCTCCAGCTCCTTCAT
  TCCCCAGCAGTACTTCTGGCTGCTGGTCCTGTCCCGGGGGCTGGTGGGCATCGGGGAGGCCAGCTACTCC
  ACCATCGCCCCCACTATCATTGGCGACCTCTTCACCAAGAACACGCGTACGCTCATGCTGTCCGTCTTCT
  ACTTCGCCATCCCACTGGGCAGTGGCCTGGGCTACATTACTGGCTCCAGCGTGAAGCAGGCAGCCGGAGA
  CTGGCACTGGGCATTGCGGGTGTCCCCTGTCCTGGGCATGATCACAGGAACACTCATCCTCATTCTGGTC
  CCAGCCACTAAAAGGGGTCATGCCGACCAGCTCGGGGACCAGCTCAAGGCCCGGACCTCATGGCTCCGAG
  ATATGAAGGCCCTGATTCGAAACCGCAGCTACGTCTTCTCCTCCCTGGCCACGTCGGCTGTCTCCTTCGC
  CACGGGGGCCCTGGGCATGTGGATCCCGCTCTACCTGCACCGCGCCCAAGTTGTGCAGAAGACAGCAGAG
  ACGTGCAACAGCCCGCCCTGTGGGGCCAAGGACAGCCTCATCTTTGGGGCCATCACCTGCTTTACGGGAT
  TTCTGGGCGTGGTCACGGGGGCAGGAGCCACGCGCTGGTGCCGCCTGAAGACCCAGCGGGCCGACCCACT
  GGTGTGTGCCGTGGGCATGCTGGGCTCTGCCATCTTCATCTGCCTGATCTTCGTGGCTGCCAAGAGCAGC
  ATCGTAGGAGCCTATATCTGTATCTTCGTCGGGGAGACGCTGCTGTTTTCTAACTGGGCCATCACTGCAG
  ACATCCTCATGTACGTGGTCATCCCCACGCGGCGCGCCACTGCCGTGGCCTTGCAGAGCTTCACCTCCCA
  CCTGCTGGGGGACGCCGGGAGCCCCTACCTCATTGGCTTTATCTCAGACCTGATCCGCCAGAGCACTAAG
  GACTCCCCGCTCTGGGAGTTCCTGAGCCTGGGCTACGCGCTCATGCTCTGCCCTTTCGTCGTGGTCCTGG
  GCGGCATGTTCTTCCTCGCCACTGCGCTCTTCTTCGTCAGCGACCGCGCCAGGGCTGAGCAGCAGGTGAA
  CCAGCTGGCGATGCCGCCCGCATCTGTGAAAGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225940 representing NM_001124758
  Red=Cloning site Green=Tags(s)
  
  MCLECASAAAGGAEEEEADAERRRRRRGAQRGAGGSGCCGARGAGGAGVSAAGDEVQTLSGSVRRAPTGP
  PGTPGTPGCAATAKGPGAQQPKPASLGRGRGAAAAILSLGNVLNYLDRYTVAGVLLDIQQHFGVKDRGAG
  LLQSVFICSFMVAAPIFGYLGDRFNRKVILSCGIFFWSAVTFSSSFIPQQYFWLLVLSRGLVGIGEASYS
  TIAPTIIGDLFTKNTRTLMLSVFYFAIPLGSGLGYITGSSVKQAAGDWHWALRVSPVLGMITGTLILILV
  PATKRGHADQLGDQLKARTSWLRDMKALIRNRSYVFSSLATSAVSFATGALGMWIPLYLHRAQVVQKTAE
  TCNSPPCGAKDSLIFGAITCFTGFLGVVTGAGATRWCRLKTQRADPLVCAVGMLGSAIFICLIFVAAKSS
  IVGAYICIFVGETLLFSNWAITADILMYVVIPTRRATAVALQSFTSHLLGDAGSPYLIGFISDLIRQSTK
  DSPLWEFLSLGYALMLCPFVVVLGGMFFLATALFFVSDRARAEQQVNQLAMPPASVKV
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001124758
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001124758.3
• RefSeq ORF: 1650 bp
• Locus ID: 124976
• UniProt ID: Q8IVW8
• Cytogenetics: 17p13.2
• Protein Families: Transmembrane
• MW: 57.9 kDa
• Gene Summary: The protein encoded by this gene is a transporter of sphingosine 1-phosphate, a secreted lipid that is important in cardiovascular, immunological, and neural development. Defects in this gene are a cause of early onset progressive hearing loss. [provided by RefSeq, Jul 2016]