ISM1 (NM_080826) Human Tagged ORF Clone

CAT#: RC222172

ISM1 (Myc-DDK-tagged)-Human isthmin 1 homolog (zebrafish) (ISM1)

Product Images

Circular map for RC222172

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  Western blot validation of overexpression lysate (Cat# LY409029) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222172 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ISM1 protein (Cat# TP322172). The protein was produced from HEK293T cells transfected with ISM1 cDNA clone (Cat# RC222172) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ISM1
• Synonyms: bA149I18.1; C20orf82; dJ1077I2.1; ISM; Isthmin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222172 representing NM_080826
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCGCCTGGCGGCCGAGCTGCTGCTGCTGCTGGGGCTGCTGCTGCTCACGCTGCACATCACCGTGC
  TGCGCGGCTCGGGAGCCGCCGACGGGCCCGACGCGGCCGCGGGCAACGCCAGCCAAGCCCAGCTGCAGAA
  TAACCTCAACGTGGGAAGTGACACCACATCAGAAACCAGCTTTTCTCTCTCCAAAGAAGCACCAAGGGAG
  CATCTGGACCACCAGGCTGCACACCAACCCTTCCCCAGACCGCGATTCCGACAAGAGACGGGGCACCCTT
  CATTGCAAAGAGATTTCCCCAGATCCTTTCTCCTTGATCTACCAAACTTTCCAGATCTTTCCAAAGCTGA
  TATCAATGGGCAGAATCCAAATATCCAGGTCACCATAGAGGTGGTCGACGGTCCTGACTCTGAAGCAGAT
  AAAGATCAGCATCCGGAGAATAAGCCCAGCTGGTCAGTCCCATCCCCCGACTGGCGGGCCTGGTGGCAGA
  GGTCCCTGTCCTTGGCCAGGGCAAACAGCGGGGACCAGGACTACAAGTACGACAGTACCTCAGACGACAG
  CAACTTCCTCAACCCCCCCAGGGGGTGGGACCATACAGCCCCAGGCCACCGGACTTTTGAAACCAAAGAT
  CAGCCAGAATATGATTCCACAGATGGCGAGGGTGACTGGAGTCTCTGGTCTGTCTGCAGCGTCACCTGCG
  GGAACGGCAACCAGAAACGGACCCGGTCTTGTGGCTACGCGTGCACTGCAACAGAATCGAGGACCTGTGA
  CCGTCCAAACTGCCCAGGAATTGAAGACACTTTTAGGACAGCTGCCACCGAAGTGAGTCTGCTTGCGGGA
  AGCGAGGAGTTTAATGCCACCAAACTGTTTGAAGTTGACACAGACAGCTGTGAGCGCTGGATGAGCTGCA
  AAAGCGAGTTCTTAAAGAAGTACATGCACAAGGTGATGAATGACCTGCCCAGCTGCCCCTGCTCCTACCC
  CACTGAGGTGGCCTACAGCACGGCCGACATCTTCGACCGCATCAAGCGCAAGGACTTCCGCTGGAAGGAC
  GCCAGCGGGCCCAAGGAGAAGCTGGAGATCTACAAGCCCACTGCCCGGTACTGCATCCGCTCCATGCTGT
  CCCTGGAGAGCACCACGCTGGCGGCACAGCACTGCTGCTACGGCGACAACATGCAGCTCATCACCAGGGG
  CAAGGGGGCGGGCACGCCCAACCTCATCAGCACCGAGTTCTCCGCGGAGCTCCACTACAAGGTGGACGTC
  CTGCCCTGGATTATCTGCAAGGGTGACTGGAGCAGGTATAACGAGGCCCGGCCTCCCAACAACGGACAGA
  AGTGCACAGAGAGCCCCTCGGACGAGGACTACATCAAGCAGTTCCAAGAGGCCAGGGAATAT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222172 representing NM_080826
  Red=Cloning site Green=Tags(s)
  
  MVRLAAELLLLLGLLLLTLHITVLRGSGAADGPDAAAGNASQAQLQNNLNVGSDTTSETSFSLSKEAPRE
  HLDHQAAHQPFPRPRFRQETGHPSLQRDFPRSFLLDLPNFPDLSKADINGQNPNIQVTIEVVDGPDSEAD
  KDQHPENKPSWSVPSPDWRAWWQRSLSLARANSGDQDYKYDSTSDDSNFLNPPRGWDHTAPGHRTFETKD
  QPEYDSTDGEGDWSLWSVCSVTCGNGNQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVSLLAG
  SEEFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVMNDLPSCPCSYPTEVAYSTADIFDRIKRKDFRWKD
  ASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYGDNMQLITRGKGAGTPNLISTEFSAELHYKVDV
  LPWIICKGDWSRYNEARPPNNGQKCTESPSDEDYIKQFQEAREY
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080826
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080826.2
• RefSeq Size: 2610 bp
• RefSeq ORF: 1395 bp
• Locus ID: 140862
• UniProt ID: B1AKI9
• Cytogenetics: 20p12.1
• MW: 51.9 kDa
• Gene Summary: Acts as an angiogenesis inhibitor.[UniProtKB/Swiss-Prot Function]