Human ISM1 (NM_080826) AAV Particle

CAT#: RC222172A1V

AAV ORF Particles, serotype AAV-2, ISM1 (Myc-DDK-tagged)-Human isthmin 1 homolog (zebrafish) (ISM1), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: ISM1
• Synonyms: bA149I18.1; C20orf82; dJ1077I2.1; ISM; Isthmin
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222172 representing NM_080826
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCGCCTGGCGGCCGAGCTGCTGCTGCTGCTGGGGCTGCTGCTGCTCACGCTGCACATCACCGTGC
  TGCGCGGCTCGGGAGCCGCCGACGGGCCCGACGCGGCCGCGGGCAACGCCAGCCAAGCCCAGCTGCAGAA
  TAACCTCAACGTGGGAAGTGACACCACATCAGAAACCAGCTTTTCTCTCTCCAAAGAAGCACCAAGGGAG
  CATCTGGACCACCAGGCTGCACACCAACCCTTCCCCAGACCGCGATTCCGACAAGAGACGGGGCACCCTT
  CATTGCAAAGAGATTTCCCCAGATCCTTTCTCCTTGATCTACCAAACTTTCCAGATCTTTCCAAAGCTGA
  TATCAATGGGCAGAATCCAAATATCCAGGTCACCATAGAGGTGGTCGACGGTCCTGACTCTGAAGCAGAT
  AAAGATCAGCATCCGGAGAATAAGCCCAGCTGGTCAGTCCCATCCCCCGACTGGCGGGCCTGGTGGCAGA
  GGTCCCTGTCCTTGGCCAGGGCAAACAGCGGGGACCAGGACTACAAGTACGACAGTACCTCAGACGACAG
  CAACTTCCTCAACCCCCCCAGGGGGTGGGACCATACAGCCCCAGGCCACCGGACTTTTGAAACCAAAGAT
  CAGCCAGAATATGATTCCACAGATGGCGAGGGTGACTGGAGTCTCTGGTCTGTCTGCAGCGTCACCTGCG
  GGAACGGCAACCAGAAACGGACCCGGTCTTGTGGCTACGCGTGCACTGCAACAGAATCGAGGACCTGTGA
  CCGTCCAAACTGCCCAGGAATTGAAGACACTTTTAGGACAGCTGCCACCGAAGTGAGTCTGCTTGCGGGA
  AGCGAGGAGTTTAATGCCACCAAACTGTTTGAAGTTGACACAGACAGCTGTGAGCGCTGGATGAGCTGCA
  AAAGCGAGTTCTTAAAGAAGTACATGCACAAGGTGATGAATGACCTGCCCAGCTGCCCCTGCTCCTACCC
  CACTGAGGTGGCCTACAGCACGGCCGACATCTTCGACCGCATCAAGCGCAAGGACTTCCGCTGGAAGGAC
  GCCAGCGGGCCCAAGGAGAAGCTGGAGATCTACAAGCCCACTGCCCGGTACTGCATCCGCTCCATGCTGT
  CCCTGGAGAGCACCACGCTGGCGGCACAGCACTGCTGCTACGGCGACAACATGCAGCTCATCACCAGGGG
  CAAGGGGGCGGGCACGCCCAACCTCATCAGCACCGAGTTCTCCGCGGAGCTCCACTACAAGGTGGACGTC
  CTGCCCTGGATTATCTGCAAGGGTGACTGGAGCAGGTATAACGAGGCCCGGCCTCCCAACAACGGACAGA
  AGTGCACAGAGAGCCCCTCGGACGAGGACTACATCAAGCAGTTCCAAGAGGCCAGGGAATAT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222172 representing NM_080826
  Red=Cloning site Green=Tags(s)
  
  MVRLAAELLLLLGLLLLTLHITVLRGSGAADGPDAAAGNASQAQLQNNLNVGSDTTSETSFSLSKEAPRE
  HLDHQAAHQPFPRPRFRQETGHPSLQRDFPRSFLLDLPNFPDLSKADINGQNPNIQVTIEVVDGPDSEAD
  KDQHPENKPSWSVPSPDWRAWWQRSLSLARANSGDQDYKYDSTSDDSNFLNPPRGWDHTAPGHRTFETKD
  QPEYDSTDGEGDWSLWSVCSVTCGNGNQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVSLLAG
  SEEFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVMNDLPSCPCSYPTEVAYSTADIFDRIKRKDFRWKD
  ASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYGDNMQLITRGKGAGTPNLISTEFSAELHYKVDV
  LPWIICKGDWSRYNEARPPNNGQKCTESPSDEDYIKQFQEAREY
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_080826
• ORF Size: 1392 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_080826.1
• RefSeq Size: 2610 bp
• RefSeq ORF: 1395 bp
• Locus ID: 140862
• UniProt ID: B1AKI9
• Cytogenetics: 20p12.1
• MW: 51.9 kDa