GAMT (NM_000156) Human Tagged ORF Clone
CAT#: RC200528
- TrueORF®
GAMT (Myc-DDK-tagged)-Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_000156" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GAMT |
Synonyms | CCDS2; HEL-S-20; PIG2; TP53I2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000156 |
ORF Size | 708 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000156.6 |
RefSeq Size | 1138 bp |
RefSeq ORF | 711 bp |
Locus ID | 2593 |
UniProt ID | Q14353 |
Cytogenetics | 19p13.3 |
Protein Families | Druggable Genome |
Protein Pathways | Arginine and proline metabolism, Glycine, serine and threonine metabolism, Metabolic pathways |
MW | 26.3 kDa |
Gene Summary | The protein encoded by this gene is a methyltransferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl donor. Defects in this gene have been implicated in neurologic syndromes and muscular hypotonia, probably due to creatine deficiency and accumulation of guanidinoacetate in the brain of affected individuals. Two transcript variants encoding different isoforms have been described for this gene. Pseudogenes of this gene are found on chromosomes 2 and 13. [provided by RefSeq, Feb 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200528L1 | Lenti-ORF clone of GAMT (Myc-DDK-tagged)-Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 1 |
USD 600.00 |
|
RC200528L2 | Lenti-ORF clone of GAMT (mGFP-tagged)-Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 1 |
USD 600.00 |
|
RC200528L3 | Lenti-ORF clone of GAMT (Myc-DDK-tagged)-Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 1 |
USD 600.00 |
|
RC200528L4 | Lenti-ORF clone of GAMT (mGFP-tagged)-Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 1 |
USD 600.00 |
|
RG200528 | GAMT (tGFP-tagged) - Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 1 |
USD 500.00 |
|
SC120079 | GAMT (untagged)-Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 1 |
USD 300.00 |
|
SC320844 | GAMT (untagged)-Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 1 |
USD 300.00 |
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