Gja3 (NM_016975) Mouse Tagged ORF Clone

CAT#: MR226893

Gja3 (Myc-DDK-tagged) - Mouse gap junction protein, alpha 3 (Gja3)

Product Images

Circular map for MR226893

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gja3
• Synonyms: Cnx46; Cx43; Cx46; Gja-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226893 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACTGGAGCTTCCTGGGGCGGCTGCTGGAGAACGCACAGGAGCACTCTACAGTCATCGGCAAAG
  TGTGGCTGACCGTGCTGTTCATCTTCCGCATTCTGGTGTTAGGGGCGGCAGCCGAGGAGGTGTGGGGCGA
  CGAGCAATCGGACTTCACCTGCAACACACAGCAGCCAGGCTGTGAGAACGTCTGCTACGACCGCGCTTTC
  CCCATTTCGCACATCCGCTTCTGGGCGCTGCAAATCATCTTCGTGTCTACGCCCACCCTCATCTATCTGG
  GCCACGTGCTACACATCGTGCGCATGGAGGAGAAGAAGAAAGAGCGGGAGGAAGAGCTGCTGAGGAGAGA
  CAACCCTCAGCACGGCCGTGGTCGCGAGCCAATGCGTACAGGGAGCCCGCGGGACCCTCCACTACGCGAT
  GACCGTGGCAAGGTGCGCATCGCAGGTGCGCTGCTGCGGACCTACGTCTTCAACATCATCTTCAAGACAC
  TCTTCGAAGTGGGGTTCATCGCGGGCCAGTACTTTCTATACGGCTTCCAGCTGCAGCCACTTTACCGCTG
  CGACCGCTGGCCCTGCCCCAACACTGTGGACTGTTTCATCTCCAGGCCCACAGAGAAGACCATCTTTGTC
  ATCTTCATGCTGGCTGTGGCCTGTGCGTCACTGGTACTCAACATGCTGGAGATTTACCACCTGGGCTGGA
  AGAAGCTCAAGCAGGGAGTTACTAACCACTTCAACCCAGATGCCTCAGAAGCCAGGCACAAGCCCTTGGA
  CCCCCTACCCACGGCCACCAGCTCTGGCCCGCCCAGCGTCTCCATCGGGTTCCCACCTTATTACACACAC
  CCTGCCTGTCCCACAGTACAGGCAAAGGCCATAGGGTTTCCTGGGGCCCCACTATCACCAGCAGACTTCA
  CAGTGGTGACTCTAAACGATGCTCAAGGCAGAAACCACCCAGTCAAACACTGCAATGGCCACCACCTGAC
  GACAGAGCAGAACTGGACCAGGCAAGTGGCAGAGCAGCAGACTCCAGCCAGCAAGCCCTCTTCAGCAGCA
  TCCAGCCCTGATGGCCGCAAGGGGCTCATTGACAGCAGTGGCAGCAGCTTACAGGAGAGTGCCTTGGTAG
  TGACGCCAGAGGAGGGGGAACAGGCTTTGGCCACCACAGTGGAGATGCACTCGCCACCGTTGGTCCTCCT
  GGACCCAGGAAGGTCCAGCAAGTCCAGCAACGGACGTGCCAGACCAGGTGACTTGGCCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226893 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDWSFLGRLLENAQEHSTVIGKVWLTVLFIFRILVLGAAAEEVWGDEQSDFTCNTQQPGCENVCYDRAF
  PISHIRFWALQIIFVSTPTLIYLGHVLHIVRMEEKKKEREEELLRRDNPQHGRGREPMRTGSPRDPPLRD
  DRGKVRIAGALLRTYVFNIIFKTLFEVGFIAGQYFLYGFQLQPLYRCDRWPCPNTVDCFISRPTEKTIFV
  IFMLAVACASLVLNMLEIYHLGWKKLKQGVTNHFNPDASEARHKPLDPLPTATSSGPPSVSIGFPPYYTH
  PACPTVQAKAIGFPGAPLSPADFTVVTLNDAQGRNHPVKHCNGHHLTTEQNWTRQVAEQQTPASKPSSAA
  SSPDGRKGLIDSSGSSLQESALVVTPEEGEQALATTVEMHSPPLVLLDPGRSSKSSNGRARPGDLAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016975
• ORF Size: 1254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016975.3, NP_058671.2
• RefSeq Size: 2696 bp
• RefSeq ORF: 1254 bp
• Locus ID: 14611
• UniProt ID: Q64448
• Cytogenetics: 14 29.82 cM
• MW: 46.3 kDa
• Gene Summary: Structural component of lens fiber gap junctions. Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane. Small molecules and ions diffuse from one cell to a neighboring cell via the central pore.[UniProtKB/Swiss-Prot Function]