Gja3 (NM_016975) Mouse Untagged Clone

CAT#: MC208554

Gja3 (untagged) - Mouse gap junction protein, alpha 3 (Gja3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gja3
• Synonyms: Cnx46; Cx43; Cx46; Gja-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208554 representing NM_016975
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACTGGAGCTTCCTGGGGCGGCTGCTGGAGAACGCACAGGAGCACTCTACAGTCATCGGCAAAG
  TGTGGCTGACCGTGCTGTTCATCTTCCGCATTCTGGTGTTAGGGGCGGCAGCCGAGGAGGTGTGGGGCGA
  CGAGCAATCGGACTTCACCTGCAACACACAGCAGCCAGGCTGTGAGAACGTCTGCTACGACCGCGCTTTC
  CCCATTTCGCACATCCGCTTCTGGGCGCTGCAAATCATCTTCGTGTCTACGCCCACCCTCATCTATCTGG
  GCCACGTGCTACACATCGTGCGCATGGAGGAGAAGAAGAAAGAGCGGGAGGAAGAGCTGCTGAGGAGAGA
  CAACCCTCAGCACGGCCGTGGTCGCGAGCCAATGCGTACAGGGAGCCCGCGGGACCCTCCACTACGCGAT
  GACCGTGGCAAGGTGCGCATCGCAGGTGCGCTGCTGCGGACCTACGTCTTCAACATCATCTTCAAGACAC
  TCTTCGAAGTGGGGTTCATCGCGGGCCAGTACTTTCTATACGGCTTCCAGCTGCAGCCACTTTACCGCTG
  CGACCGCTGGCCCTGCCCCAACACTGTGGACTGTTTCATCTCCAGGCCCACAGAGAAGACCATCTTTGTC
  ATCTTCATGCTGGCTGTGGCCTGTGCGTCACTGGTACTCAACATGCTGGAGATTTACCACCTGGGCTGGA
  AGAAGCTCAAGCAGGGAGTTACTAACCACTTCAACCCAGATGCCTCAGAAGCCAGGCACAAGCCCTTGGA
  CCCCCTACCCACGGCCACCAGCTCTGGCCCGCCCAGCGTCTCCATCGGGTTCCCACCTTATTACACACAC
  CCTGCCTGTCCCACAGTACAGGCAAAGGCCATAGGGTTTCCTGGGGCCCCACTATCACCAGCAGACTTCA
  CAGTGGTGACTCTAAACGATGCTCAAGGCAGAAACCACCCAGTCAAACACTGCAATGGCCACCACCTGAC
  GACAGAGCAGAACTGGACCAGGCAAGTGGCAGAGCAGCAGACTCCAGCCAGCAAGCCCTCTTCAGCAGCA
  TCCAGCCCTGATGGCCGCAAGGGGCTCATTGACAGCAGTGGCAGCAGCTTACAGGAGAGTGCCTTGGTAG
  TGACGCCAGAGGAGGGGGAACAGGCTTTGGCCACCACAGTGGAGATGCACTCGCCACCGTTGGTCCTCCT
  GGACCCAGGAAGGTCCAGCAAGTCCAGCAACGGACGTGCCAGACCAGGTGACTTGGCCATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016975
• Insert Size: 1254 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_016975.3, NP_058671.2
• RefSeq Size: 2696 bp
• RefSeq ORF: 1254 bp
• Locus ID: 14611
• UniProt ID: Q64448
• Cytogenetics: 14 29.82 cM
• Gene Summary: Structural component of lens fiber gap junctions. Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane. Small molecules and ions diffuse from one cell to a neighboring cell via the central pore.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.