Bcan (NM_007529) Mouse Tagged ORF Clone

CAT#: MR211054

Bcan (Myc-DDK-tagged) - Mouse brevican (Bcan), transcript variant 1

Product Images

Circular map for MR211054

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bcan
• Synonyms: Cspg7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211054 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCCACTGCTTCTGTCCCTGCTGGCCGCTCTGGTCCTGACCCAAGCCCCTGCCGCCCTCGCTGATG
  ACCTGAAAGAAGACAGCTCGGAGGATCGAGCCTTCCGCGTGCGCATCGGTGCCACGCAGCTGCGGGGCGT
  GCTGGGCGGTGCCCTGGCCATCCCATGCCACGTCCACCACCTGCGGCCGCCGCACAGTCGCCGGGCCGCG
  CCGGGTTTTCCCCGGGTCAAGTGGACCTTCCTGTCCGGGGACCGGGAGGTAGAGGTACTGGTGGCTCGTG
  GGCTGCGCGTCAAGGTAAACGAAGCCTACCGGTTCCGCGTGGCGCTGCCTGCCTACCCCGCATCGCTCAC
  GGATGTGTCTCTAGTATTGAGCGAACTGCGGCCCAATGATTCCGGGGTCTATCGCTGCGAGGTCCAGCAC
  GGTATCGACGACAGCAGTGATGCTGTGGAGGTCAAGGTCAAAGGGGTCGTCTTCCTCTACAGAGAGGGCT
  CTGCGCGCTATGCTTTCTCCTTTGCTGGAGCCCAGGAAGCCTGCGCTCGCATAGGAGCCCGAATCGCCAC
  CCCGGAGCAGCTCTATGCTGCCTACCTCGGCGGCTATGAGCAGTGTGATGCAGGCTGGCTGTCCGACCAA
  ACTGTGAGGTACCCCATCCAGAACCCACGAGAGGCCTGCTCTGGAGACATGGATGGCTATCCTGGCGTGC
  GGAACTACGGAGTGGTGGGTCCTGATGATCTCTATGATGTCTACTGTTATGCCGAAGACCTAAATGGAGA
  ACTGTTCCTAGGCGCCCCTCCCAGCAAGCTGACATGGGAGGAGGCTCGGGACTACTGTCTGGAACGTGGT
  GCACAGATCGCTAGCACAGGCCAGCTGTACGCAGCCTGGAATGGTGGCCTGGACAGATGTAGCCCTGGCT
  GGCTGGCTGATGGCAGCGTGCGCTATCCCATCATCACACCCAGCCAACGCTGTGGGGGCGGCCTGCCAGG
  AGTCAAGACCCTCTTCCTCTTTCCCAACCAGACTGGCTTCCCCAGCAAGCAGAACCGCTTCAATGTCTAC
  TGCTTCCGAGACTCTGCCCATCCCTCTGCTTCCTCTGAGGCCTCTAGCCCAGCCTCAGATGGACTTGAGG
  CCATTGTCACAGTGACAGAAAAGCTGGAGGAACTGCAGCTGCCTCAGGAAGCGATGGAGAGCGAGTCTCG
  TGGGGCCATCTACTCCATCCCCATCTCAGAAGATGGGGGAGGAGGAAGCTCCACCCCAGAAGACCCAGCA
  GAGGCCCCCAGGACTCCGCTAGAATCGGAAACCCAATCCATTGCACCACCTACCGAGTCCTCAGAAGAGG
  AAGGCGTAGCCCTGGAGGAAGAAGAAAGATTCAAAGACTTGGAGGCTCTGGAGGAAGAGAAGGAGCAGGA
  GGACCTGTGGGTGTGGCCCAGAGAGCTCAGCAGCCCTCTCCCTACTGGCTCAGAAACAGAGCATTCACTC
  TCCCAGGTGTCCCCACCAGCCCAGGCAGTTCTACAGCTGGGTGCCTCACCTTCTCCTGGGCCTCCAAGGG
  TCCGTGGACCGCCTGCAGAGACTTTGCTCCCCCCGAGGGAGGGAAGCCCCACATCTACTCCTGGTGGGGC
  AAGAGAAGTAGGGGGGGAAACTGGGAGCCCTGAGCTCTCTGGGGTTCCTCGAGAGAGCGAGGAGGCAGGG
  AGCTCCAGCTTGGAGGATGGCCCTTCCCTACTTCCAGCTACATGGGCCCCTGTGGGTCCCAGGGAGCTGG
  AGACCCCCTCAGAAGAGAAGTCTGGAAGAACTGTCCTGGCAGGCACCTCAGTGCAGGCCCAGCCAGTGCT
  GCCCACCGACAGTGCCAGCCACGGTGGAGTGGCTGTGGCTCCCTCATCAGGTGACTGTATCCCCAGCCCC
  TGCCACAATGGTGGGACATGCTTGGAGGAGAAGGAGGGTTTCCGCTGCCTATGTTTGCCAGGCTATGGGG
  GGGACCTGTGCGATGTTGGCCTTCATTTCTGCAGCCCTGGCTGGGAGGCCTTCCAGGGAGCCTGCTACAA
  GCACTTTTCCACACGAAGGAGTTGGGAGGAGGCAGAAAGTCAGTGCCGAGCGCTAGGTGCTCATCTGACC
  AGCATCTGCACCCCTGAGGAGCAAGACTTTGTCAACGATCGATACCGGGAGTACCAGTGGATTGGGCTCA
  ATGACAGGACCATCGAGGGTGACTTCTTGTGGTCAGATGGTGCCCCTCTGCTCTATGAAAACTGGAACCC
  TGGGCAGCCTGACAGCTACTTCCTGTCTGGGGAGAACTGTGTGGTCATGGTGTGGCATGACCAGGGACAG
  TGGAGTGATGTGCCCTGCAACTACCATCTATCCTACACCTGCAAGATGGGGCTTGTGTCCTGTGGGCCTC
  CACCACAGCTACCCCTGGCTCAAATATTTGGTCGCCCTCGGCTGCGCTACGCGGTGGATACTGTGCTTCG
  ATATCGATGCCGAGACGGGCTGGCTCAGCGCAACCTGCCGTTGATCCGCTGCCAGGAGAATGGGCTTTGG
  GAGGCCCCTCAGATTTCCTGTGTACCCCGGAGGCCTGGCCGTGCTCTGCGCTCCATGGACGCCCCAGAAG
  GACCACGGGGACAGCTCTCGAGGCACAGGAAGGCACCGTTGACACCGCCCTCCAGTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211054 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIPLLLSLLAALVLTQAPAALADDLKEDSSEDRAFRVRIGATQLRGVLGGALAIPCHVHHLRPPHSRRAA
  PGFPRVKWTFLSGDREVEVLVARGLRVKVNEAYRFRVALPAYPASLTDVSLVLSELRPNDSGVYRCEVQH
  GIDDSSDAVEVKVKGVVFLYREGSARYAFSFAGAQEACARIGARIATPEQLYAAYLGGYEQCDAGWLSDQ
  TVRYPIQNPREACSGDMDGYPGVRNYGVVGPDDLYDVYCYAEDLNGELFLGAPPSKLTWEEARDYCLERG
  AQIASTGQLYAAWNGGLDRCSPGWLADGSVRYPIITPSQRCGGGLPGVKTLFLFPNQTGFPSKQNRFNVY
  CFRDSAHPSASSEASSPASDGLEAIVTVTEKLEELQLPQEAMESESRGAIYSIPISEDGGGGSSTPEDPA
  EAPRTPLESETQSIAPPTESSEEEGVALEEEERFKDLEALEEEKEQEDLWVWPRELSSPLPTGSETEHSL
  SQVSPPAQAVLQLGASPSPGPPRVRGPPAETLLPPREGSPTSTPGGAREVGGETGSPELSGVPRESEEAG
  SSSLEDGPSLLPATWAPVGPRELETPSEEKSGRTVLAGTSVQAQPVLPTDSASHGGVAVAPSSGDCIPSP
  CHNGGTCLEEKEGFRCLCLPGYGGDLCDVGLHFCSPGWEAFQGACYKHFSTRRSWEEAESQCRALGAHLT
  SICTPEEQDFVNDRYREYQWIGLNDRTIEGDFLWSDGAPLLYENWNPGQPDSYFLSGENCVVMVWHDQGQ
  WSDVPCNYHLSYTCKMGLVSCGPPPQLPLAQIFGRPRLRYAVDTVLRYRCRDGLAQRNLPLIRCQENGLW
  EAPQISCVPRRPGRALRSMDAPEGPRGQLSRHRKAPLTPPSSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007529
• ORF Size: 2652 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007529.2
• RefSeq Size: 3267 bp
• RefSeq ORF: 2652 bp
• Locus ID: 12032
• UniProt ID: Q61361
• Cytogenetics: 3 38.78 cM
• MW: 95.8 kDa
• Gene Summary: May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans.[UniProtKB/Swiss-Prot Function]