Bcan (NM_007529) Mouse Tagged ORF Clone
CAT#: MG211054
- TrueORF®
Bcan (tGFP-tagged) - Mouse brevican (Bcan)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_007529" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Bcan |
Synonyms | Cspg7 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG211054 representing NM_007529
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCCACTGCTTCTGTCCCTGCTGGCCGCTCTGGTCCTGACCCAAGCCCCTGCCGCCCTCGCTGATG ACCTGAAAGAAGACAGCTCGGAGGATCGAGCCTTCCGCGTGCGCATCGGTGCCACGCAGCTGCGGGGCGT GCTGGGCGGTGCCCTGGCCATCCCATGCCACGTCCACCACCTGCGGCCGCCGCACAGTCGCCGGGCCGCG CCGGGTTTTCCCCGGGTCAAGTGGACCTTCCTGTCCGGGGACCGGGAGGTAGAGGTACTGGTGGCTCGTG GGCTGCGCGTCAAGGTAAACGAAGCCTACCGGTTCCGCGTGGCGCTGCCTGCCTACCCCGCATCGCTCAC GGATGTGTCTCTAGTATTGAGCGAACTGCGGCCCAATGATTCCGGGGTCTATCGCTGCGAGGTCCAGCAC GGTATCGACGACAGCAGTGATGCTGTGGAGGTCAAGGTCAAAGGGGTCGTCTTCCTCTACAGAGAGGGCT CTGCGCGCTATGCTTTCTCCTTTGCTGGAGCCCAGGAAGCCTGCGCTCGCATAGGAGCCCGAATCGCCAC CCCGGAGCAGCTCTATGCTGCCTACCTCGGCGGCTATGAGCAGTGTGATGCAGGCTGGCTGTCCGACCAA ACTGTGAGGTACCCCATCCAGAACCCACGAGAGGCCTGCTCTGGAGACATGGATGGCTATCCTGGCGTGC GGAACTACGGAGTGGTGGGTCCTGATGATCTCTATGATGTCTACTGTTATGCCGAAGACCTAAATGGAGA ACTGTTCCTAGGCGCCCCTCCCAGCAAGCTGACATGGGAGGAGGCTCGGGACTACTGTCTGGAACGTGGT GCACAGATCGCTAGCACAGGCCAGCTGTACGCAGCCTGGAATGGTGGCCTGGACAGATGTAGCCCTGGCT GGCTGGCTGATGGCAGCGTGCGCTATCCCATCATCACACCCAGCCAACGCTGTGGGGGCGGCCTGCCAGG AGTCAAGACCCTCTTCCTCTTTCCCAACCAGACTGGCTTCCCCAGCAAGCAGAACCGCTTCAATGTCTAC TGCTTCCGAGACTCTGCCCATCCCTCTGCTTCCTCTGAGGCCTCTAGCCCAGCCTCAGATGGACTTGAGG CCATTGTCACAGTGACAGAAAAGCTGGAGGAACTGCAGCTGCCTCAGGAAGCGATGGAGAGCGAGTCTCG TGGGGCCATCTACTCCATCCCCATCTCAGAAGATGGGGGAGGAGGAAGCTCCACCCCAGAAGACCCAGCA GAGGCCCCCAGGACTCCGCTAGAATCGGAAACCCAATCCATTGCACCACCTACCGAGTCCTCAGAAGAGG AAGGCGTAGCCCTGGAGGAAGAAGAAAGATTCAAAGACTTGGAGGCTCTGGAGGAAGAGAAGGAGCAGGA GGACCTGTGGGTGTGGCCCAGAGAGCTCAGCAGCCCTCTCCCTACTGGCTCAGAAACAGAGCATTCACTC TCCCAGGTGTCCCCACCAGCCCAGGCAGTTCTACAGCTGGGTGCCTCACCTTCTCCTGGGCCTCCAAGGG TCCGTGGACCGCCTGCAGAGACTTTGCTCCCCCCGAGGGAGGGAAGCCCCACATCTACTCCTGGTGGGGC AAGAGAAGTAGGGGGGGAAACTGGGAGCCCTGAGCTCTCTGGGGTTCCTCGAGAGAGCGAGGAGGCAGGG AGCTCCAGCTTGGAGGATGGCCCTTCCCTACTTCCAGCTACATGGGCCCCTGTGGGTCCCAGGGAGCTGG AGACCCCCTCAGAAGAGAAGTCTGGAAGAACTGTCCTGGCAGGCACCTCAGTGCAGGCCCAGCCAGTGCT GCCCACCGACAGTGCCAGCCACGGTGGAGTGGCTGTGGCTCCCTCATCAGGTGACTGTATCCCCAGCCCC TGCCACAATGGTGGGACATGCTTGGAGGAGAAGGAGGGTTTCCGCTGCCTATGTTTGCCAGGCTATGGGG GGGACCTGTGCGATGTTGGCCTTCATTTCTGCAGCCCTGGCTGGGAGGCCTTCCAGGGAGCCTGCTACAA GCACTTTTCCACACGAAGGAGTTGGGAGGAGGCAGAAAGTCAGTGCCGAGCGCTAGGTGCTCATCTGACC AGCATCTGCACCCCTGAGGAGCAAGACTTTGTCAACGATCGATACCGGGAGTACCAGTGGATTGGGCTCA ATGACAGGACCATCGAGGGTGACTTCTTGTGGTCAGATGGTGCCCCTCTGCTCTATGAAAACTGGAACCC TGGGCAGCCTGACAGCTACTTCCTGTCTGGGGAGAACTGTGTGGTCATGGTGTGGCATGACCAGGGACAG TGGAGTGATGTGCCCTGCAACTACCATCTATCCTACACCTGCAAGATGGGGCTTGTGTCCTGTGGGCCTC CACCACAGCTACCCCTGGCTCAAATATTTGGTCGCCCTCGGCTGCGCTACGCGGTGGATACTGTGCTTCG ATATCGATGCCGAGACGGGCTGGCTCAGCGCAACCTGCCGTTGATCCGCTGCCAGGAGAATGGGCTTTGG GAGGCCCCTCAGATTTCCTGTGTACCCCGGAGGCCTGGCCGTGCTCTGCGCTCCATGGACGCCCCAGAAG GACCACGGGGACAGCTCTCGAGGCACAGGAAGGCACCGTTGACACCGCCCTCCAGTCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211054 representing NM_007529
Red=Cloning site Green=Tags(s) MIPLLLSLLAALVLTQAPAALADDLKEDSSEDRAFRVRIGATQLRGVLGGALAIPCHVHHLRPPHSRRAA PGFPRVKWTFLSGDREVEVLVARGLRVKVNEAYRFRVALPAYPASLTDVSLVLSELRPNDSGVYRCEVQH GIDDSSDAVEVKVKGVVFLYREGSARYAFSFAGAQEACARIGARIATPEQLYAAYLGGYEQCDAGWLSDQ TVRYPIQNPREACSGDMDGYPGVRNYGVVGPDDLYDVYCYAEDLNGELFLGAPPSKLTWEEARDYCLERG AQIASTGQLYAAWNGGLDRCSPGWLADGSVRYPIITPSQRCGGGLPGVKTLFLFPNQTGFPSKQNRFNVY CFRDSAHPSASSEASSPASDGLEAIVTVTEKLEELQLPQEAMESESRGAIYSIPISEDGGGGSSTPEDPA EAPRTPLESETQSIAPPTESSEEEGVALEEEERFKDLEALEEEKEQEDLWVWPRELSSPLPTGSETEHSL SQVSPPAQAVLQLGASPSPGPPRVRGPPAETLLPPREGSPTSTPGGAREVGGETGSPELSGVPRESEEAG SSSLEDGPSLLPATWAPVGPRELETPSEEKSGRTVLAGTSVQAQPVLPTDSASHGGVAVAPSSGDCIPSP CHNGGTCLEEKEGFRCLCLPGYGGDLCDVGLHFCSPGWEAFQGACYKHFSTRRSWEEAESQCRALGAHLT SICTPEEQDFVNDRYREYQWIGLNDRTIEGDFLWSDGAPLLYENWNPGQPDSYFLSGENCVVMVWHDQGQ WSDVPCNYHLSYTCKMGLVSCGPPPQLPLAQIFGRPRLRYAVDTVLRYRCRDGLAQRNLPLIRCQENGLW EAPQISCVPRRPGRALRSMDAPEGPRGQLSRHRKAPLTPPSSL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007529 |
ORF Size | 2649 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007529.2 |
RefSeq Size | 3153 bp |
RefSeq ORF | 2652 bp |
Locus ID | 12032 |
UniProt ID | Q61361 |
Cytogenetics | 3 38.78 cM |
Gene Summary | May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203629 | Bcan (untagged) - Mouse brevican (Bcan), transcript variant 1, (10ug) |
USD 809.00 |
|
MR211054 | Bcan (Myc-DDK-tagged) - Mouse brevican (Bcan), transcript variant 1 |
USD 809.00 |
|
MR211054L3 | Lenti ORF clone of Bcan (Myc-DDK-tagged) - Mouse brevican (Bcan), transcript variant 1 |
USD 1,109.00 |
|
MR211054L4 | Lenti ORF clone of Bcan (mGFP-tagged) - Mouse brevican (Bcan), transcript variant 1 |
USD 1,109.00 |
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