Cd300lf (NM_145634) Mouse Tagged ORF Clone

CAT#: MR204865

Cd300lf (Myc-DDK-tagged) - Mouse CD300 antigen like family member F (Cd300lf), transcript variant 2

Product Images

Circular map for MR204865

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd300lf
• Synonyms: CLIM1; CLM-1; CLM1; Digr2; F730004D16Rik; IgSF13; IREM1; LMIR3; Pigr3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204865 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATTTGTCATTGCTGGTCCCCTTTCTCTTCTGGATCACAGGCTGCTGCACGGCTGAGGATCCAGTCA
  CAGGTCCAGAGGAGGTGAGCGGTCAGGAGCAGGGCTCCTTGACAGTGCAGTGCCGATATACCTCAGGCTG
  GAAGGATTACAAGAAGTACTGGTGCCAAGGAGTTCCTCAGAGATCATGTAAGACTCTTGTTGAAACCGAT
  GCATCAGAGCAGCTGGTGAAGAAGAACCGTGTGTCCATCAGGGACAACCAGAGAGACTTCATCTTCACAG
  TGACCATGGAGGATCTGAGGATGAGCGATGCTGGCATTTACTGGTGTGGAATTACGAAAGGTGGACTTGA
  TCCCATGTTTAAAGTTACTGTGAACATTGGCCCAGTGCCAACCATGCCCCCCATCACCTCCACCACCACC
  ATCTTCACAGTGACAACCACAGTAAAAGAGACCAGCATGTTTCCAACGCTGACTAGCTACTACTCTGATA
  ACGGGCATGGCGGTGGTGACAGTGGCGGTGGTGAAGATGGCGTCGGTGATGGGTTTCTGGATCTCAGTGT
  GCTCCTCCCAGTCATCTCTGCAGTCCTGTTGCTTCTCCTGTTGGTGGCCTCGCTCTTTGCTTGGAGGATG
  GTGAGGAGACAGAAGAAAGCTGCTGGGCCACCATCAGAGCAGGCACAGTCTCTGGAGGGTGATCTCTGTT
  ATGCAGACCTGTCCCTGAAGCAGCCCAGAACCTCCCCTGGCTCCTCTTGGAAAAAGGGCTCCTCCATGTC
  CTCCTCTGGCAAGGACCACCAAGAGGAAGTGGAATATGTCACCATGGCTCCCTTTCCCAGGGAGGAGGTT
  TCATATGCCGCTCTGACTTTGGCCGGCTTGGGTCAGGAGCCTACTTATGGCAATACTGGCTGCCCCATCA
  CCCACGTTCCCAGGACAGGCCTTGAAGAGGAGACCACAGAGTACAGCAGCATCAGGAGGCCCTTGCCTGC
  AGCCATGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204865 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHLSLLVPFLFWITGCCTAEDPVTGPEEVSGQEQGSLTVQCRYTSGWKDYKKYWCQGVPQRSCKTLVETD
  ASEQLVKKNRVSIRDNQRDFIFTVTMEDLRMSDAGIYWCGITKGGLDPMFKVTVNIGPVPTMPPITSTTT
  IFTVTTTVKETSMFPTLTSYYSDNGHGGGDSGGGEDGVGDGFLDLSVLLPVISAVLLLLLLVASLFAWRM
  VRRQKKAAGPPSEQAQSLEGDLCYADLSLKQPRTSPGSSWKKGSSMSSSGKDHQEEVEYVTMAPFPREEV
  SYAALTLAGLGQEPTYGNTGCPITHVPRTGLEEETTEYSSIRRPLPAAMP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145634
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145634.3
• RefSeq Size: 1864 bp
• RefSeq ORF: 993 bp
• Locus ID: 246746
• UniProt ID: Q6SJQ7
• Cytogenetics: 11 E2
• MW: 36 kDa
• Gene Summary: Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:17438331). Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells (PubMed:21865548). Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis (PubMed:26768664). Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide which acts as a ligand (PubMed:23123064). May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling (PubMed:26124135). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2 (By similarity). Inhibits osteoclast formation (PubMed:14662855). Induces macrophage cell death upon engagement (PubMed:18097021).[UniProtKB/Swiss-Prot Function]