Cd300lf (NM_145634) Mouse Untagged Clone

CAT#: MC204586

Cd300lf (untagged) - Mouse CD300 antigen like family member F (Cd300lf), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cd300lf
• Synonyms: CLIM1; CLM-1; CLM1; Digr2; F730004D16Rik; IgSF13; IREM1; LMIR3; Pigr3
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057864
  GACCAGGAGACGCAGGAGTGGAGCATGTAGCCTGTTCTCGCTGGCAGGCTCCACCAAGGTGACCCGGTGT GAGAAGATGCATTTGTCATTGCTGGTCCCCTTTCTCTTCTGGATCACAGGCTGCTGCACGGCTGAGGATC CAGTCACAGGTCCAGAGGAGGTGAGCGGTCAGGAGCAGGGCTCCTTGACAGTGCAGTGCCGATATACCTC AGGCTGGAAGGATTACAAGAAGTACTGGTGCCAAGGAGTTCCTCAGAGATCATGTAAGACTCTTGTTGAA ACCGATGCATCAGAGCAGCTGGTGAAGAAGAACCGTGTGTCCATCAGGGACAACCAGAGAGACTTCATCT TCACAGTGACCATGGAGGATCTGAGGATGAGCGATGCTGGCATTTACTGGTGTGGAATTACGAAAGGTGG ACTTGATCCCATGTTTAAAGTTACTGTGAACATTGGCCCAGTGCCAACCATGCCCCCCATCACCTCCACC ACCACCATCTTCACAGTGACAACCACAGTAAAAGAGACCAGCATGTTTCCAACGCTGACTAGCTACTACT CTGATAACGGGCATGGCGGTGGTGACAGTGGCGGTGGTGAAGATGGCGTCGGTGATGGGTTTCTGGATCT CAGTGTGCTCCTCCCAGTCATCTCTGCAGTCCTGTTGCTTCTCCTGTTGGTGGCCTCGCTCTTTGCTTGG AGGATGGTGAGGAGACAGAAGAAAGCTGCTGGGCCACCATCAGAGCAGGCACAGTCTCTGGAGGGTGATC TCTGTTATGCAGACCTGTCCCTGAAGCAGCCCAGAACCTCCCCTGGCTCCTCTTGGAAAAAGGGCTCCTC CATGTCCTCCTCTGGCAAGGACCACCAAGAGGAAGTGGAATATGTCACCATGGCTCCCTTTCCCAGGGAG GAGGTTTCATATGCCGCTCTGACTTTGGCCGGCTTGGGTCAGGAGCCTACTTATGGCAATACTGGCTGCC CCATCACCCACGTTCCCAGGACAGGCCTTGAAGAGGAGACCACAGAGTACAGCAGCATCAGGAGGCCCTT GCCTGCAGCCATGCCTTAATCTTGGTCTCTGAAGGCGGCTTGGAGCATGGATCTTTACATCTGCCTCTGT ACCTGCTTCCTTACCCGGCCCAGCTGGTGACTGGAACTCTGTCCATCCGTCTCTCATGGCCATCAGCTCT ACCTTGCTTGAGCTTGGAGTTCAACCTCAGGGAGTTCCAGGGAATTAAGGCTCCTTCCACATCCCCACTT ATAGCCAATGTACCTTGGAAGGTACCAGGCAGGCTGCTTCAGGGATGCTGTGTAAATCGTATCAACGATG ACAATAATAGCAATCAACCTTTATTTATTGTTTTTCATATGGGTTTCCCCCGCCCCTCCCCCCAGGACAT TTTCATCCTCATACCCATAATCTGTAAATTATTTTATACAAAGAAAGATAAGTACATATGTGTTTAAGTT AGAGTTTTGAGATGAGTCTGTATTATCCAAGTAGGCCCAAAATATAACCACAAGGGTCTGTTTTAAGGAG GAAGAGAGAGAGAGAGAGAGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAG AGAGAGAACAACAGAAGCAGAAGGTGGAGGGATGCAGAAGGAGTCAGGATGTTGCACATCCAGCAGCCAG CAAGGCCAAGTATCAGACTGTTCTGTTCCGTGAGCGGAGCACTGGGTGGCGATTAGGTCTGCCAGTGTCA AGGGTTGGGTGGAGTTGAATTGATTGTGTACTTTCAGAATTGTAGAAGAATAAAGTTGTATTGTTTTTAA AAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_145634
• Insert Size: 993 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057864, AAH57864
• RefSeq Size: 1834 bp
• RefSeq ORF: 993 bp
• Locus ID: 246746
• UniProt ID: Q6SJQ7
• Cytogenetics: 11 E2
• Gene Summary: Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:17438331). Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells (PubMed:21865548). Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis (PubMed:26768664). Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide which acts as a ligand (PubMed:23123064). May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling (PubMed:26124135). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2 (By similarity). Inhibits osteoclast formation (PubMed:14662855). Induces macrophage cell death upon engagement (PubMed:18097021).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses a different splice site in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.